Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4)
Antioxidant activity of carotenoids may shift into prooxidant activity, depending on several factors, such as oxygen tension, carotenoid concentration, and interactions with other antioxidants. This study was conducted to prepare DMEM culture medium containing high concentrations of lutein and β-...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/10653/ http://irep.iium.edu.my/10653/ http://irep.iium.edu.my/10653/1/Improvement_of_solubilization_and_uptake_of_dietary_antioxidants_in_human_hepatocellular_%282009%29.pdf |
| Summary: | Antioxidant activity of carotenoids may shift into prooxidant activity, depending on several
factors, such as oxygen tension, carotenoid concentration, and interactions with other
antioxidants. This study was conducted to prepare DMEM culture medium containing high
concentrations of lutein and β-carotene for evaluation of the anti- and pro-oxidant activities and
their dependency on concentrations of the carotenoids. We successfully developed a new method
to prepare DMEM medium containing higher concentration of lutein and beta-carotene. In the
new method, lutein and β-carotene were solubilized in 5% THF. However, the concentration of
THF was then reduced almost completely through evaporation. THF concentration checking was
done using a headspace gas chromatography. Using this evaporation method, results showed
that medium containing up to 90μM could be obtained, compared to a maximum of 15uM of
lutein by using a conventional method, while THF was reduced to a non-toxic level as low as
0.01%. This finding allowed the use of high concentrations of the carotenoids for antioxidative
activity study. Cellular uptake and antioxidative activity in HepG2 cells of the carotenoids were
examined under a wide range of their concentrations. |
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