Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4)

Antioxidant activity of carotenoids may shift into prooxidant activity, depending on several factors, such as oxygen tension, carotenoid concentration, and interactions with other antioxidants. This study was conducted to prepare DMEM culture medium containing high concentrations of lutein and β-...

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Main Authors:	Jaswir, Irwandi, Nagao, Akihiko
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/10653/ http://irep.iium.edu.my/10653/ http://irep.iium.edu.my/10653/1/Improvement_of_solubilization_and_uptake_of_dietary_antioxidants_in_human_hepatocellular_%282009%29.pdf

id	iium-10653
recordtype	eprints
spelling	iium-106532011-12-15T11:25:58Z http://irep.iium.edu.my/10653/ Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4) Jaswir, Irwandi Nagao, Akihiko QD Chemistry Antioxidant activity of carotenoids may shift into prooxidant activity, depending on several factors, such as oxygen tension, carotenoid concentration, and interactions with other antioxidants. This study was conducted to prepare DMEM culture medium containing high concentrations of lutein and β-carotene for evaluation of the anti- and pro-oxidant activities and their dependency on concentrations of the carotenoids. We successfully developed a new method to prepare DMEM medium containing higher concentration of lutein and beta-carotene. In the new method, lutein and β-carotene were solubilized in 5% THF. However, the concentration of THF was then reduced almost completely through evaporation. THF concentration checking was done using a headspace gas chromatography. Using this evaporation method, results showed that medium containing up to 90μM could be obtained, compared to a maximum of 15uM of lutein by using a conventional method, while THF was reduced to a non-toxic level as low as 0.01%. This finding allowed the use of high concentrations of the carotenoids for antioxidative activity study. Cellular uptake and antioxidative activity in HepG2 cells of the carotenoids were examined under a wide range of their concentrations. 2009 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/10653/1/Improvement_of_solubilization_and_uptake_of_dietary_antioxidants_in_human_hepatocellular_%282009%29.pdf Jaswir, Irwandi and Nagao, Akihiko (2009) Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4). In: International Palm Oil Congress (PIPOC 2009) , 9-12 November 2009, Kuala Lumpur Convention Centre (KLCC), KL, Malaysia. http://www.mpob.gov.my
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Jaswir, Irwandi Nagao, Akihiko Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4)
description	Antioxidant activity of carotenoids may shift into prooxidant activity, depending on several factors, such as oxygen tension, carotenoid concentration, and interactions with other antioxidants. This study was conducted to prepare DMEM culture medium containing high concentrations of lutein and β-carotene for evaluation of the anti- and pro-oxidant activities and their dependency on concentrations of the carotenoids. We successfully developed a new method to prepare DMEM medium containing higher concentration of lutein and beta-carotene. In the new method, lutein and β-carotene were solubilized in 5% THF. However, the concentration of THF was then reduced almost completely through evaporation. THF concentration checking was done using a headspace gas chromatography. Using this evaporation method, results showed that medium containing up to 90μM could be obtained, compared to a maximum of 15uM of lutein by using a conventional method, while THF was reduced to a non-toxic level as low as 0.01%. This finding allowed the use of high concentrations of the carotenoids for antioxidative activity study. Cellular uptake and antioxidative activity in HepG2 cells of the carotenoids were examined under a wide range of their concentrations.
format	Conference or Workshop Item
author	Jaswir, Irwandi Nagao, Akihiko
author_facet	Jaswir, Irwandi Nagao, Akihiko
author_sort	Jaswir, Irwandi
title	Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4)
title_short	Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4)
title_full	Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4)
title_fullStr	Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4)
title_full_unstemmed	Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4)
title_sort	improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (hepg2) cell line (pdnp4)
publishDate	2009
url	http://irep.iium.edu.my/10653/ http://irep.iium.edu.my/10653/ http://irep.iium.edu.my/10653/1/Improvement_of_solubilization_and_uptake_of_dietary_antioxidants_in_human_hepatocellular_%282009%29.pdf
first_indexed	2023-09-18T20:20:03Z
last_indexed	2023-09-18T20:20:03Z
_version_	1777408045874151424

Improvement of solubilization and uptake of dietary antioxidants in human hepatocellular liver carcinoma (HepG2) cell line (PDNP4)

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