High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector
A reverse phase high performance liquid chromatographic method for the detection of the neurotransmitters namely serotonin, tryptophan and dopamine in human blood samples has been developed. Spiked standard samples were analysed using a fluorescence detector after chromatographic separation. For the...
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2010
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| Online Access: | http://irep.iium.edu.my/10676/ http://irep.iium.edu.my/10676/1/51._HIGH_PERFORMANCE_LIQUID_CHROMATOGRAPHIC...._%28A.B.M._Helal_et_al.%29.pdf |
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iium-106762013-10-17T04:19:10Z http://irep.iium.edu.my/10676/ High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector Helaluddin , Abul Bashar Mohammed Saeed , Mohammad Khan, Umeed Ali QD Chemistry RS Pharmacy and materia medica A reverse phase high performance liquid chromatographic method for the detection of the neurotransmitters namely serotonin, tryptophan and dopamine in human blood samples has been developed. Spiked standard samples were analysed using a fluorescence detector after chromatographic separation. For the analytes a working range of 2.5 ng/mL to 25 ng/mL were found suitable for the application. For chromatographic separation C18 type of column showed the better separation results and was used for the study. Mobile phase was optimised to 97% phosphate buffer and ion pairing reagent (pH adjusted to 5.5) together with 3% acetonitrile. Sample preparation step was found to be more effective with protein precipitation method than the solvent extraction method. Two protein precipitation methods were compared and found that perchloric acid method was more suitable than that of acetonitrile method. Intra and inter day precision of the analytes were determined and the coefficient of variation (CV) were less 15% which is within the acceptable limit of standard guidelines. Specificity of the analytes was established by comparing the standard peaks with the blank plasma chromatogram. The analysis results showed that the method is specific, accurate and reliable for the quantification of the analytes in human samples. 2010-12-16 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/10676/1/51._HIGH_PERFORMANCE_LIQUID_CHROMATOGRAPHIC...._%28A.B.M._Helal_et_al.%29.pdf Helaluddin , Abul Bashar Mohammed and Saeed , Mohammad and Khan, Umeed Ali (2010) High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector. In: 3rd International Conference on Science & Technology : Applications in Industry and Education (ICSTIE 2010), 16-17 Dec 2010, Penang. |
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Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QD Chemistry RS Pharmacy and materia medica |
| spellingShingle |
QD Chemistry RS Pharmacy and materia medica Helaluddin , Abul Bashar Mohammed Saeed , Mohammad Khan, Umeed Ali High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector |
| description |
A reverse phase high performance liquid chromatographic method for the detection of the neurotransmitters namely serotonin, tryptophan and dopamine in human blood samples has been developed. Spiked standard samples were analysed using a fluorescence detector after chromatographic separation. For the analytes a working range of 2.5 ng/mL to 25 ng/mL were found suitable for the application. For chromatographic separation C18 type of column showed the better separation results and was used for the study. Mobile phase was optimised to 97% phosphate buffer and ion pairing reagent (pH adjusted to 5.5) together with 3% acetonitrile. Sample preparation step was found to be more effective with protein precipitation method than the solvent extraction method. Two protein precipitation methods were compared and found that perchloric acid method was more suitable than that of acetonitrile method. Intra and inter day precision of the analytes were determined and the coefficient of variation (CV) were less 15% which is within the acceptable limit of standard guidelines. Specificity of the analytes was established by comparing the standard peaks with the blank plasma chromatogram. The analysis results showed that the method is specific, accurate and reliable for the quantification of the analytes in human samples. |
| format |
Conference or Workshop Item |
| author |
Helaluddin , Abul Bashar Mohammed Saeed , Mohammad Khan, Umeed Ali |
| author_facet |
Helaluddin , Abul Bashar Mohammed Saeed , Mohammad Khan, Umeed Ali |
| author_sort |
Helaluddin , Abul Bashar Mohammed |
| title |
High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector |
| title_short |
High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector |
| title_full |
High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector |
| title_fullStr |
High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector |
| title_full_unstemmed |
High performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector |
| title_sort |
high performance liquid chromatographic method for the detection of some neurotransmitters in blood samples using fluorescence detector |
| publishDate |
2010 |
| url |
http://irep.iium.edu.my/10676/ http://irep.iium.edu.my/10676/1/51._HIGH_PERFORMANCE_LIQUID_CHROMATOGRAPHIC...._%28A.B.M._Helal_et_al.%29.pdf |
| first_indexed |
2023-09-18T20:20:04Z |
| last_indexed |
2023-09-18T20:20:04Z |
| _version_ |
1777408047038070784 |