Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice

Light microscopic (LM) and transmission electron microscopic (TEM) observations were performed in the kidneys of oral contraceptive treated mice. Experimental groups were given 0.1 ml of olive oil containing 0.015 fraction of (Neogynon) tablet daily. Control groups were give olive oil alone. Five e...

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Main Authors:	Al-Ani, Imad Matloub Dally, Noor Al-Deen, Jinan A., Kashmola, Muhanad Ali Hamdon
Format:	Article
Language:	English
Published:	Department of Anatomy, National University of Singapore 2009
Subjects:	QM Human anatomy QP Physiology RB Pathology
Online Access:	http://irep.iium.edu.my/10689/ http://irep.iium.edu.my/10689/ http://irep.iium.edu.my/10689/1/Effectof_Contraciptive_pills.pdf

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spelling	iium-106892015-12-18T03:24:27Z http://irep.iium.edu.my/10689/ Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice Al-Ani, Imad Matloub Dally Noor Al-Deen, Jinan A. Kashmola, Muhanad Ali Hamdon QM Human anatomy QP Physiology RB Pathology Light microscopic (LM) and transmission electron microscopic (TEM) observations were performed in the kidneys of oral contraceptive treated mice. Experimental groups were given 0.1 ml of olive oil containing 0.015 fraction of (Neogynon) tablet daily. Control groups were give olive oil alone. Five experimental and three control animals were scarifi ed after 1, 3, 6, 9, and 12 estrus cycles. Glomerulosclerosis, hyaline arteriopathy associated with focal areas of interstitial fi brosis were observed in the fi rst cycle. Mild hypertrophy of juxtaglomerular apparatus “JGA” appeared in the 3rd cycle with increased granularity of the JGCs. Proliferative-sclerosing stages of focal glomerulonephritis were observed and were more advanced in cycle twelve. By transmission electron microscope, there were slight changes in the glomeruli during cycle one. These evident gradually developed in cycle three, thus the glomeruli showed disordered capillary loop occlusion. In cycle six, capillary lumens were occluded by protuberance projection. There were hyperactivity and proliferation of mesangial cells and electron-dense material deposits in glomerular basal lamina; these deposits were accompanied with electron-lucent materials appeared in cycle 9 behind swelled mesangial cells indicating hyperactivity and proliferative cells which showed abundant RER and mitochondria. These lesions were correlated with hyper-granularity of JGCs and hypertrophied JGA. The JGGs appeared in cycle 12 as heterogenized size. These changes indicate that abuse of oral contraceptives is relatively unsafe, especially after long-term of high-dose usage. Department of Anatomy, National University of Singapore 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/10689/1/Effectof_Contraciptive_pills.pdf Al-Ani, Imad Matloub Dally and Noor Al-Deen, Jinan A. and Kashmola, Muhanad Ali Hamdon (2009) Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice. Annals of Microscopy, 9. pp. 63-72. ISSN 0219-2209 http://www.microscopy.org.sg/journal.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QM Human anatomy QP Physiology RB Pathology
spellingShingle	QM Human anatomy QP Physiology RB Pathology Al-Ani, Imad Matloub Dally Noor Al-Deen, Jinan A. Kashmola, Muhanad Ali Hamdon Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice
description	Light microscopic (LM) and transmission electron microscopic (TEM) observations were performed in the kidneys of oral contraceptive treated mice. Experimental groups were given 0.1 ml of olive oil containing 0.015 fraction of (Neogynon) tablet daily. Control groups were give olive oil alone. Five experimental and three control animals were scarifi ed after 1, 3, 6, 9, and 12 estrus cycles. Glomerulosclerosis, hyaline arteriopathy associated with focal areas of interstitial fi brosis were observed in the fi rst cycle. Mild hypertrophy of juxtaglomerular apparatus “JGA” appeared in the 3rd cycle with increased granularity of the JGCs. Proliferative-sclerosing stages of focal glomerulonephritis were observed and were more advanced in cycle twelve. By transmission electron microscope, there were slight changes in the glomeruli during cycle one. These evident gradually developed in cycle three, thus the glomeruli showed disordered capillary loop occlusion. In cycle six, capillary lumens were occluded by protuberance projection. There were hyperactivity and proliferation of mesangial cells and electron-dense material deposits in glomerular basal lamina; these deposits were accompanied with electron-lucent materials appeared in cycle 9 behind swelled mesangial cells indicating hyperactivity and proliferative cells which showed abundant RER and mitochondria. These lesions were correlated with hyper-granularity of JGCs and hypertrophied JGA. The JGGs appeared in cycle 12 as heterogenized size. These changes indicate that abuse of oral contraceptives is relatively unsafe, especially after long-term of high-dose usage.
format	Article
author	Al-Ani, Imad Matloub Dally Noor Al-Deen, Jinan A. Kashmola, Muhanad Ali Hamdon
author_facet	Al-Ani, Imad Matloub Dally Noor Al-Deen, Jinan A. Kashmola, Muhanad Ali Hamdon
author_sort	Al-Ani, Imad Matloub Dally
title	Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice
title_short	Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice
title_full	Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice
title_fullStr	Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice
title_full_unstemmed	Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice
title_sort	light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice
publisher	Department of Anatomy, National University of Singapore
publishDate	2009
url	http://irep.iium.edu.my/10689/ http://irep.iium.edu.my/10689/ http://irep.iium.edu.my/10689/1/Effectof_Contraciptive_pills.pdf
first_indexed	2023-09-18T20:20:04Z
last_indexed	2023-09-18T20:20:04Z
_version_	1777408047659876352

Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice

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