The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia

Community-acquired pneumonia (CAP) is still a major cause of morbidity and mortality especially to children and compromised hosts, such as the old and those with underlying chronic diseases. Knowledge of pathogens causing CAP constitutes the basis for selection of antimicrobial treatment. Previou...

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Main Authors:	Mustafa Mahmoud, Mohammed Imad Al-Deen, Al-Marzooq, Farah Ibrahim, How, Soon Hin
Format:	Article
Language:	English
Published:	Malaysian Society of Parasitology and Tropical Medicine 2011
Subjects:	QR Microbiology RC Internal medicine
Online Access:	http://irep.iium.edu.my/11116/ http://irep.iium.edu.my/11116/ http://irep.iium.edu.my/11116/1/531_-_544_Al-Marzooq_F_%285%29.pdf

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spelling	iium-111162013-04-11T03:03:29Z http://irep.iium.edu.my/11116/ The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia Mustafa Mahmoud, Mohammed Imad Al-Deen Al-Marzooq, Farah Ibrahim How, Soon Hin QR Microbiology RC Internal medicine Community-acquired pneumonia (CAP) is still a major cause of morbidity and mortality especially to children and compromised hosts, such as the old and those with underlying chronic diseases. Knowledge of pathogens causing CAP constitutes the basis for selection of antimicrobial treatment. Previous data have shown that etiological agents can be identified in only up to 50% of patients, but this figure can be improved by using polymerase chain reaction (PCR). This study was designed to evaluate multiplex real-time PCR as a method for rapid differential detection of five bacterial causes of CAP (Streptococcus pneumoniae, Burkholderia pseudomallei and atypical bacterial pathogens namely Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila) in CAP patients attending Hospital Tengku Ampuan Afzan (HTAA)/ Kuantan, Pahang, Malaysia. Two previously developed multiplex real-time PCR assays, duplex for the differential detection of S. pneumoniae and B. pseudomallei and triplex for the atypical bacterial pathogens, were used to detect a bacterial cause of CAP in blood and respiratory samples. Thus, 46 blood and 45 respiratory samples collected from 46 adult CAP patients admitted to HTAA were analysed by multiplex real-time PCR assays and conventional methods. The microbial etiology of CAP could be established for 39.1% (18/46) of CAP patients by conventional methods and this was increased to 65.2% (30/46) with the additional use of real-time PCR. The most frequently detected pathogens were S. pneumoniae (21.7% - all by PCR alone), Klebsiella pneumoniae (17.3%), B. pseudomallei (13% - 83% of them positive by PCR alone and 17% by both culture and PCR), Pseudomonas aeruginosa (6.5%), M. pneumoniae (6.5% - all by serology), C. pneumoniae (4.3% - all positive by both PCR and serology), L. pneumophila (2.1% - all by PCR alone), Escherichia coli (4.3%). Haemophilus infuenzae, Acinetobacter lwoffii and Acinetobacter baumannii were detected by conventional methods (2.1% for each). Malaysian Society of Parasitology and Tropical Medicine 2011-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/11116/1/531_-_544_Al-Marzooq_F_%285%29.pdf Mustafa Mahmoud, Mohammed Imad Al-Deen and Al-Marzooq, Farah Ibrahim and How, Soon Hin (2011) The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia. Tropical Biomedicine, 28 (3). pp. 531-544. ISSN 0127-5720, OCLC: 12391449 http://www.msptm.org/journal.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology RC Internal medicine
spellingShingle	QR Microbiology RC Internal medicine Mustafa Mahmoud, Mohammed Imad Al-Deen Al-Marzooq, Farah Ibrahim How, Soon Hin The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia
description	Community-acquired pneumonia (CAP) is still a major cause of morbidity and mortality especially to children and compromised hosts, such as the old and those with underlying chronic diseases. Knowledge of pathogens causing CAP constitutes the basis for selection of antimicrobial treatment. Previous data have shown that etiological agents can be identified in only up to 50% of patients, but this figure can be improved by using polymerase chain reaction (PCR). This study was designed to evaluate multiplex real-time PCR as a method for rapid differential detection of five bacterial causes of CAP (Streptococcus pneumoniae, Burkholderia pseudomallei and atypical bacterial pathogens namely Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila) in CAP patients attending Hospital Tengku Ampuan Afzan (HTAA)/ Kuantan, Pahang, Malaysia. Two previously developed multiplex real-time PCR assays, duplex for the differential detection of S. pneumoniae and B. pseudomallei and triplex for the atypical bacterial pathogens, were used to detect a bacterial cause of CAP in blood and respiratory samples. Thus, 46 blood and 45 respiratory samples collected from 46 adult CAP patients admitted to HTAA were analysed by multiplex real-time PCR assays and conventional methods. The microbial etiology of CAP could be established for 39.1% (18/46) of CAP patients by conventional methods and this was increased to 65.2% (30/46) with the additional use of real-time PCR. The most frequently detected pathogens were S. pneumoniae (21.7% - all by PCR alone), Klebsiella pneumoniae (17.3%), B. pseudomallei (13% - 83% of them positive by PCR alone and 17% by both culture and PCR), Pseudomonas aeruginosa (6.5%), M. pneumoniae (6.5% - all by serology), C. pneumoniae (4.3% - all positive by both PCR and serology), L. pneumophila (2.1% - all by PCR alone), Escherichia coli (4.3%). Haemophilus infuenzae, Acinetobacter lwoffii and Acinetobacter baumannii were detected by conventional methods (2.1% for each).
format	Article
author	Mustafa Mahmoud, Mohammed Imad Al-Deen Al-Marzooq, Farah Ibrahim How, Soon Hin
author_facet	Mustafa Mahmoud, Mohammed Imad Al-Deen Al-Marzooq, Farah Ibrahim How, Soon Hin
author_sort	Mustafa Mahmoud, Mohammed Imad Al-Deen
title	The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia
title_short	The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia
title_full	The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia
title_fullStr	The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia
title_full_unstemmed	The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia
title_sort	use of multiplex real-time pcr improves the detection of the bacterial etiology of community acquired pneumonia
publisher	Malaysian Society of Parasitology and Tropical Medicine
publishDate	2011
url	http://irep.iium.edu.my/11116/ http://irep.iium.edu.my/11116/ http://irep.iium.edu.my/11116/1/531_-_544_Al-Marzooq_F_%285%29.pdf
first_indexed	2023-09-18T20:20:28Z
last_indexed	2023-09-18T20:20:28Z
_version_	1777408072982986752

The use of multiplex real-time PCR improves the detection of the bacterial etiology of community acquired pneumonia

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