New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture

Antioxidant activity of carotenoids may shift into prooxidant activity, depending on several factors, such as oxygen tension, carotenoid concentration, and interactions with other antioxidants. This study was conducted to prepare DMEM culture medium containing high concentrations of lutein and 13-c...

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Main Authors:	Jaswir, Irwandi, Nagao, Akihiko
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/11918/ http://irep.iium.edu.my/11918/1/ICCMB_2010.pdf

id	iium-11918
recordtype	eprints
spelling	iium-119182014-06-04T13:38:01Z http://irep.iium.edu.my/11918/ New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture Jaswir, Irwandi Nagao, Akihiko TP248.13 Biotechnology Antioxidant activity of carotenoids may shift into prooxidant activity, depending on several factors, such as oxygen tension, carotenoid concentration, and interactions with other antioxidants. This study was conducted to prepare DMEM culture medium containing high concentrations of lutein and 13-carotene for evaluation ofthe anti- and pro-oxidant activities and their dependency on concentrations of the carotenoids. We succesfully developed a new method to prepare DMEM medium containing higher concentration of lutein and beta-carotene. In the new method, lutein and l3-carotene were solubilized in 5% THF. However, the concentration of THF was then reduced almost completely through evaporation. THF concentration checking was done using a headspace gas chromatography. Using this evaporation method, results showed that medium containing up to 90~M could be obtained, compared to a maximum of 15uM of lutein by using a conventional method, while THF was reduced to a nontoxic level as low as 0.01 %. This finding allowed the use of high concentrations of the carotenoids for antioxidative activity study. Cellular uptake and antioxidative activity in HepG2 cells of the carotenoids were examined under a wide range of their concentrations. 2010 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/11918/1/ICCMB_2010.pdf Jaswir, Irwandi and Nagao, Akihiko (2010) New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture. In: International Conference on Cellular & Molecular Bioengineering (ICCMB), 2-4 August, 2010, Singapore.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Jaswir, Irwandi Nagao, Akihiko New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture
description	Antioxidant activity of carotenoids may shift into prooxidant activity, depending on several factors, such as oxygen tension, carotenoid concentration, and interactions with other antioxidants. This study was conducted to prepare DMEM culture medium containing high concentrations of lutein and 13-carotene for evaluation ofthe anti- and pro-oxidant activities and their dependency on concentrations of the carotenoids. We succesfully developed a new method to prepare DMEM medium containing higher concentration of lutein and beta-carotene. In the new method, lutein and l3-carotene were solubilized in 5% THF. However, the concentration of THF was then reduced almost completely through evaporation. THF concentration checking was done using a headspace gas chromatography. Using this evaporation method, results showed that medium containing up to 90~M could be obtained, compared to a maximum of 15uM of lutein by using a conventional method, while THF was reduced to a nontoxic level as low as 0.01 %. This finding allowed the use of high concentrations of the carotenoids for antioxidative activity study. Cellular uptake and antioxidative activity in HepG2 cells of the carotenoids were examined under a wide range of their concentrations.
format	Conference or Workshop Item
author	Jaswir, Irwandi Nagao, Akihiko
author_facet	Jaswir, Irwandi Nagao, Akihiko
author_sort	Jaswir, Irwandi
title	New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture
title_short	New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture
title_full	New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture
title_fullStr	New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture
title_full_unstemmed	New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture
title_sort	new method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (hepg2) cell culture
publishDate	2010
url	http://irep.iium.edu.my/11918/ http://irep.iium.edu.my/11918/1/ICCMB_2010.pdf
first_indexed	2023-09-18T20:21:11Z
last_indexed	2023-09-18T20:21:11Z
_version_	1777408117369208832

New method for improving solubilization and uptake of lutein and beta-carotene in human hepatocellular liver carcinoma (HepG2) cell culture

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