A Cryopreservatlon method of Sclzaphyllurn commune local strains

Schizophyllan commune fsolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains (wheat, corn and paddy). The grains were incubated for 7 days at room temperature. After the grains were fully covered with myceHa, they we...

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Main Authors:	Mohd Kamal, Irnada Syuhaidah, Mohd Nor Rashid, Noraswati, Mel, Maizirwan
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/11991/ http://irep.iium.edu.my/11991/1/Microbiology_Congress%231.pdf

id	iium-11991
recordtype	eprints
spelling	iium-119912015-09-09T03:05:58Z http://irep.iium.edu.my/11991/ A Cryopreservatlon method of Sclzaphyllurn commune local strains Mohd Kamal, Irnada Syuhaidah Mohd Nor Rashid, Noraswati Mel, Maizirwan TP248.13 Biotechnology Schizophyllan commune fsolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains (wheat, corn and paddy). The grains were incubated for 7 days at room temperature. After the grains were fully covered with myceHa, they were place into a cryo-vial with three different. treatments, 10% glycerol, sterile distilled water and no cryoprotectants. AU of the treatments were frozen in liquid nitrogen for 7 days followed by thawing (cooling) at 30°C for 30 min. The grains were inoculated onto PDA agar to observe the viability (rate of survival) of the seeds. The radius of each grain with different treatments was measured. Results showed that all treatments showed 100% viability aft.er 48 hours and wheat wit.h no cryoprotectants showed the highest growth rate. This indicated that the grains have mycelial protective properties. In this study, a cryopreservation process was simplified as opposed to the conventional method in which 10% glycerol was used as the cryoprotectant. This study promotes an alternative method for cryopreservalion which is more economical as no cryoprotectants were required. 2011-12-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/11991/1/Microbiology_Congress%231.pdf Mohd Kamal, Irnada Syuhaidah and Mohd Nor Rashid, Noraswati and Mel, Maizirwan (2011) A Cryopreservatlon method of Sclzaphyllurn commune local strains. In: International Congress of the Malaysian Society for Microbiology 2011, 8-11 December 2011, Penang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohd Kamal, Irnada Syuhaidah Mohd Nor Rashid, Noraswati Mel, Maizirwan A Cryopreservatlon method of Sclzaphyllurn commune local strains
description	Schizophyllan commune fsolates were collected from rubber plantations in Selangor and Perak area in Malaysia. Spawns were prepared using three different grains (wheat, corn and paddy). The grains were incubated for 7 days at room temperature. After the grains were fully covered with myceHa, they were place into a cryo-vial with three different. treatments, 10% glycerol, sterile distilled water and no cryoprotectants. AU of the treatments were frozen in liquid nitrogen for 7 days followed by thawing (cooling) at 30°C for 30 min. The grains were inoculated onto PDA agar to observe the viability (rate of survival) of the seeds. The radius of each grain with different treatments was measured. Results showed that all treatments showed 100% viability aft.er 48 hours and wheat wit.h no cryoprotectants showed the highest growth rate. This indicated that the grains have mycelial protective properties. In this study, a cryopreservation process was simplified as opposed to the conventional method in which 10% glycerol was used as the cryoprotectant. This study promotes an alternative method for cryopreservalion which is more economical as no cryoprotectants were required.
format	Conference or Workshop Item
author	Mohd Kamal, Irnada Syuhaidah Mohd Nor Rashid, Noraswati Mel, Maizirwan
author_facet	Mohd Kamal, Irnada Syuhaidah Mohd Nor Rashid, Noraswati Mel, Maizirwan
author_sort	Mohd Kamal, Irnada Syuhaidah
title	A Cryopreservatlon method of Sclzaphyllurn commune local strains
title_short	A Cryopreservatlon method of Sclzaphyllurn commune local strains
title_full	A Cryopreservatlon method of Sclzaphyllurn commune local strains
title_fullStr	A Cryopreservatlon method of Sclzaphyllurn commune local strains
title_full_unstemmed	A Cryopreservatlon method of Sclzaphyllurn commune local strains
title_sort	cryopreservatlon method of sclzaphyllurn commune local strains
publishDate	2011
url	http://irep.iium.edu.my/11991/ http://irep.iium.edu.my/11991/1/Microbiology_Congress%231.pdf
first_indexed	2023-09-18T20:21:13Z
last_indexed	2023-09-18T20:21:13Z
_version_	1777408119282860032

A Cryopreservatlon method of Sclzaphyllurn commune local strains

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