Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model

Abstract Objective: This study aims to induce surgical osteoarthritis in a sheep model by excision of the medial meniscus and anterior cruciate ligament of the knee joint. Methods: Osteoarthritis (OA) was induced unilaterally in the knee joint of five adult male sheep by total excision of the med...

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Main Authors:	Alfaqeh, Hamoud Hussein Mohammed, Casim, Samsudin Osman, Saim, Aminuddin, Idrus, Ruszymah
Format:	Conference or Workshop Item
Language:	English
Published:	2008
Subjects:	RD701 Orthopedics
Online Access:	http://irep.iium.edu.my/12114/ http://irep.iium.edu.my/12114/1/OA_induction_poster.pdf

id	iium-12114
recordtype	eprints
spelling	iium-121142012-07-17T01:08:14Z http://irep.iium.edu.my/12114/ Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model Alfaqeh, Hamoud Hussein Mohammed Casim, Samsudin Osman Saim, Aminuddin Idrus, Ruszymah RD701 Orthopedics Abstract Objective: This study aims to induce surgical osteoarthritis in a sheep model by excision of the medial meniscus and anterior cruciate ligament of the knee joint. Methods: Osteoarthritis (OA) was induced unilaterally in the knee joint of five adult male sheep by total excision of the medial meniscus and resection of the anterior cruciate ligament (ACL). After a recovery period of 3 weeks, all animals were exercised once daily by running on a hard surface for a distance of 100 meters. Following that the animals were allowed free movement in a confined environment. The animals were sacrificed six weeks after operation. The femoral condyle and the proximal tibial plateau of operated and none operated legs were remove. The interior part of the knee joint was graded according to ICRS grading system. Histological evaluation done by Haematoxylin & Eosin (H&E) and Safranin-O staining. Result: The ICRS grading clearly showed define OA changes as compared to control. H&E staining demonstrated a deep defect on the study area with evidence of inflammation. Safranin-O staining showed absence of cartilage layer. Conclusion: Total excision of meniscus and ACL results in surgically induced osteoarthritis within a short period of time (six weeks) in a sheep model. This study hopes to open new horizons in evaluating treatment for osteoarthritis especially in the area of cell and tissue based therapy. Keywords: Osteoarthritis, Anterior Cruciate Ligament; Medial Meniscus; Sheep; Tissue Engineering 2008-07 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/12114/1/OA_induction_poster.pdf Alfaqeh, Hamoud Hussein Mohammed and Casim, Samsudin Osman and Saim, Aminuddin and Idrus, Ruszymah (2008) Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model. In: Malaysian Tissue Engineering & Regenerative Medicine Scientific Meeting, 22-23 July 2008, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RD701 Orthopedics
spellingShingle	RD701 Orthopedics Alfaqeh, Hamoud Hussein Mohammed Casim, Samsudin Osman Saim, Aminuddin Idrus, Ruszymah Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model
description	Abstract Objective: This study aims to induce surgical osteoarthritis in a sheep model by excision of the medial meniscus and anterior cruciate ligament of the knee joint. Methods: Osteoarthritis (OA) was induced unilaterally in the knee joint of five adult male sheep by total excision of the medial meniscus and resection of the anterior cruciate ligament (ACL). After a recovery period of 3 weeks, all animals were exercised once daily by running on a hard surface for a distance of 100 meters. Following that the animals were allowed free movement in a confined environment. The animals were sacrificed six weeks after operation. The femoral condyle and the proximal tibial plateau of operated and none operated legs were remove. The interior part of the knee joint was graded according to ICRS grading system. Histological evaluation done by Haematoxylin & Eosin (H&E) and Safranin-O staining. Result: The ICRS grading clearly showed define OA changes as compared to control. H&E staining demonstrated a deep defect on the study area with evidence of inflammation. Safranin-O staining showed absence of cartilage layer. Conclusion: Total excision of meniscus and ACL results in surgically induced osteoarthritis within a short period of time (six weeks) in a sheep model. This study hopes to open new horizons in evaluating treatment for osteoarthritis especially in the area of cell and tissue based therapy. Keywords: Osteoarthritis, Anterior Cruciate Ligament; Medial Meniscus; Sheep; Tissue Engineering
format	Conference or Workshop Item
author	Alfaqeh, Hamoud Hussein Mohammed Casim, Samsudin Osman Saim, Aminuddin Idrus, Ruszymah
author_facet	Alfaqeh, Hamoud Hussein Mohammed Casim, Samsudin Osman Saim, Aminuddin Idrus, Ruszymah
author_sort	Alfaqeh, Hamoud Hussein Mohammed
title	Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model
title_short	Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model
title_full	Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model
title_fullStr	Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model
title_full_unstemmed	Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model
title_sort	medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model
publishDate	2008
url	http://irep.iium.edu.my/12114/ http://irep.iium.edu.my/12114/1/OA_induction_poster.pdf
first_indexed	2023-09-18T20:21:20Z
last_indexed	2023-09-18T20:21:20Z
_version_	1777408127541444608

Medial meniscectomy and anterior cruciate ligament resection result in osteoarthritis in a sheep model

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