Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7

This study was conducted to investigate the potential of marine actinomycetes isolated from Malaysian waters as producers of biologically active compounds. Streptomyces sp. UKMCC_PT7 was isolated from seawater collected in Pulau Tinggi, Johor. This bacterium was found to produce yellow diffusible pi...

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Main Authors:	Zainal Abidin, Zaima Azira, Ahmad, Asmat, Latip, Jalifah, Usup, Gires
Format:	Conference or Workshop Item
Language:	English English
Published:	2011
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/12132/ http://irep.iium.edu.my/12132/2/poster_langkawi.pdf http://irep.iium.edu.my/12132/5/ABSTRACT_skam_24.pdf

id	iium-12132
recordtype	eprints
spelling	iium-121322012-01-06T06:01:25Z http://irep.iium.edu.my/12132/ Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7 Zainal Abidin, Zaima Azira Ahmad, Asmat Latip, Jalifah Usup, Gires Q Science (General) This study was conducted to investigate the potential of marine actinomycetes isolated from Malaysian waters as producers of biologically active compounds. Streptomyces sp. UKMCC_PT7 was isolated from seawater collected in Pulau Tinggi, Johor. This bacterium was found to produce yellow diffusible pigment when growing on ISP3 medium. Preliminary screening showed that this yellow diffusible pigment possessed antibacterial properties. Ethyl acetate extraction was then carried out on agar containing the yellow pigment followed by separation of compounds using reverse-phase HPLC which led to the isolation of three metabolites; PT7_27, PT7_30 and PT7_31. All compounds exhibited UV maximal absorption at 433 nm with masses of 1272, 1269 and 1255 respectively. 1H and 13C/APT NMR data revealed that these compounds are actinomycin derivatives which consist of phenoxazinone chromophore with two lactonised pentapeptide attached via amide linkage. One of the compounds was established as Actinomycin D (C62H86N12O16), a widely used antitumor drug to treat Wilms’ tumor, Ewing’s sarcoma, rhabdomysarcoma and gestational trophoblastic disease. This study further demonstrated the unique capabilities of marine Streptomyces of producing more than one antibiotic simultaneously. 2011-11-21 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/12132/2/poster_langkawi.pdf application/pdf en http://irep.iium.edu.my/12132/5/ABSTRACT_skam_24.pdf Zainal Abidin, Zaima Azira and Ahmad, Asmat and Latip, Jalifah and Usup, Gires (2011) Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7. In: Simposium Kimia Analisis Malaysia, 21-23 November 2011, Langkawi, Malaysia. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Zainal Abidin, Zaima Azira Ahmad, Asmat Latip, Jalifah Usup, Gires Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7
description	This study was conducted to investigate the potential of marine actinomycetes isolated from Malaysian waters as producers of biologically active compounds. Streptomyces sp. UKMCC_PT7 was isolated from seawater collected in Pulau Tinggi, Johor. This bacterium was found to produce yellow diffusible pigment when growing on ISP3 medium. Preliminary screening showed that this yellow diffusible pigment possessed antibacterial properties. Ethyl acetate extraction was then carried out on agar containing the yellow pigment followed by separation of compounds using reverse-phase HPLC which led to the isolation of three metabolites; PT7_27, PT7_30 and PT7_31. All compounds exhibited UV maximal absorption at 433 nm with masses of 1272, 1269 and 1255 respectively. 1H and 13C/APT NMR data revealed that these compounds are actinomycin derivatives which consist of phenoxazinone chromophore with two lactonised pentapeptide attached via amide linkage. One of the compounds was established as Actinomycin D (C62H86N12O16), a widely used antitumor drug to treat Wilms’ tumor, Ewing’s sarcoma, rhabdomysarcoma and gestational trophoblastic disease. This study further demonstrated the unique capabilities of marine Streptomyces of producing more than one antibiotic simultaneously.
format	Conference or Workshop Item
author	Zainal Abidin, Zaima Azira Ahmad, Asmat Latip, Jalifah Usup, Gires
author_facet	Zainal Abidin, Zaima Azira Ahmad, Asmat Latip, Jalifah Usup, Gires
author_sort	Zainal Abidin, Zaima Azira
title	Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7
title_short	Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7
title_full	Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7
title_fullStr	Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7
title_full_unstemmed	Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7
title_sort	isolation of actinomycin derivatives from diffusible pigment of streptomyces sp. ukmcc_pt7
publishDate	2011
url	http://irep.iium.edu.my/12132/ http://irep.iium.edu.my/12132/2/poster_langkawi.pdf http://irep.iium.edu.my/12132/5/ABSTRACT_skam_24.pdf
first_indexed	2023-09-18T20:21:22Z
last_indexed	2023-09-18T20:21:22Z
_version_	1777408129181417472

Isolation of actinomycin derivatives from diffusible pigment of Streptomyces sp. UKMCC_PT7

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