Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines

Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines

The expression pattern of functional members of the metallothionein (MT) gene family was studied in the haematopoietic precursor cell lines, K562, DAMI, MEG-01, and ELF-153 in order to strengthen the proposed function of MT in differentiation. Cells were cultured in RPMI 1640 with 10% (v/v) foetal c...

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Main Authors: Maghdooni Bagheri, Pegah, Rahman, Mohammad Tariqur, Van Soest, Sofie, De Ley, Marc
Format: Article
Language:English
English
Published: Elsevier Science B.V. 2009
Subjects:
QP Physiology
Online Access:http://irep.iium.edu.my/12434/
http://irep.iium.edu.my/12434/
http://irep.iium.edu.my/12434/
http://irep.iium.edu.my/12434/1/S0946672X09000248
http://irep.iium.edu.my/12434/2/Differential_quantitative_zinc-induced_expression_of_human_metallothionein_isogenes_in_haematopoietic_precursor_cell_lines.pdf
id iium-12434
recordtype eprints
spelling iium-124342012-02-01T03:29:55Z http://irep.iium.edu.my/12434/ Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines Maghdooni Bagheri, Pegah Rahman, Mohammad Tariqur Van Soest, Sofie De Ley, Marc QP Physiology The expression pattern of functional members of the metallothionein (MT) gene family was studied in the haematopoietic precursor cell lines, K562, DAMI, MEG-01, and ELF-153 in order to strengthen the proposed function of MT in differentiation. Cells were cultured in RPMI 1640 with 10% (v/v) foetal calf serum, with or without different zinc supplements. Expression of MT isogenes was analysed by quantitative real-time PCR (RT-PCR) using mRNA extracted from cultured cells. The more mature K562, DAMI, and MEG-01 cell lines exhibited transcription of all MT isogenes, except MT-3 and MT-4. Relative quantitative expression of MT isogenes in the mature cell lines such as K562, DAMI, and MEG-01 was higher than in the immature ELF-153 cell line. Immunohistochemical staining (IHC) reveals an increased MT protein biosynthesis in more mature cell lines such as K562, DAMI and MEG-01 greater than in the immature ELF-153 cell line. Real-time PCR and immunohistochemical staining for investigating the effect of phorbol ester and hemin (haematopoietic differentiation stimuli) on expression of MT isogenes in K562 cells reveals that phorbol ester induces increased MT transcription and biosynthesis. Therefore, to our knowledge, the role of MT in differentiation in human haematopoietic precursor cell lines is here reported for the first time. Elsevier Science B.V. 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/12434/1/S0946672X09000248 application/pdf en http://irep.iium.edu.my/12434/2/Differential_quantitative_zinc-induced_expression_of_human_metallothionein_isogenes_in_haematopoietic_precursor_cell_lines.pdf Maghdooni Bagheri, Pegah and Rahman, Mohammad Tariqur and Van Soest, Sofie and De Ley, Marc (2009) Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines. Journal of Trace Elements in Medicine and Biology, 23 (2). pp. 124-31. ISSN 0946-672X (P), 1878-3252 (O) http://www.sciencedirect.com/science/article/pii/S0946672X09000248 10.1016/j.jtemb.2009.02.003
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic QP Physiology
spellingShingle QP Physiology
Maghdooni Bagheri, Pegah
Rahman, Mohammad Tariqur
Van Soest, Sofie
De Ley, Marc
Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines
description The expression pattern of functional members of the metallothionein (MT) gene family was studied in the haematopoietic precursor cell lines, K562, DAMI, MEG-01, and ELF-153 in order to strengthen the proposed function of MT in differentiation. Cells were cultured in RPMI 1640 with 10% (v/v) foetal calf serum, with or without different zinc supplements. Expression of MT isogenes was analysed by quantitative real-time PCR (RT-PCR) using mRNA extracted from cultured cells. The more mature K562, DAMI, and MEG-01 cell lines exhibited transcription of all MT isogenes, except MT-3 and MT-4. Relative quantitative expression of MT isogenes in the mature cell lines such as K562, DAMI, and MEG-01 was higher than in the immature ELF-153 cell line. Immunohistochemical staining (IHC) reveals an increased MT protein biosynthesis in more mature cell lines such as K562, DAMI and MEG-01 greater than in the immature ELF-153 cell line. Real-time PCR and immunohistochemical staining for investigating the effect of phorbol ester and hemin (haematopoietic differentiation stimuli) on expression of MT isogenes in K562 cells reveals that phorbol ester induces increased MT transcription and biosynthesis. Therefore, to our knowledge, the role of MT in differentiation in human haematopoietic precursor cell lines is here reported for the first time.
format Article
author Maghdooni Bagheri, Pegah
Rahman, Mohammad Tariqur
Van Soest, Sofie
De Ley, Marc
author_facet Maghdooni Bagheri, Pegah
Rahman, Mohammad Tariqur
Van Soest, Sofie
De Ley, Marc
author_sort Maghdooni Bagheri, Pegah
title Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines
title_short Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines
title_full Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines
title_fullStr Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines
title_full_unstemmed Differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines
title_sort differential quantitative zinc-induced expression of human metallothionein isogenes in haematopoietic precursor cell lines
publisher Elsevier Science B.V.
publishDate 2009
url http://irep.iium.edu.my/12434/
http://irep.iium.edu.my/12434/
http://irep.iium.edu.my/12434/
http://irep.iium.edu.my/12434/1/S0946672X09000248
http://irep.iium.edu.my/12434/2/Differential_quantitative_zinc-induced_expression_of_human_metallothionein_isogenes_in_haematopoietic_precursor_cell_lines.pdf
first_indexed 2023-09-18T20:21:39Z
last_indexed 2023-09-18T20:21:39Z
_version_ 1777408146555273216

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