Cell rupture of recombinant escherichia coli using high pressure homogenizer

Cell rupture is one of the earlier steps in downstream processing which are required for the recovery of biological products that are located inside cells. Cells could be disrupted either by using chemicals or mechanical method. In this study, cell rupture was carried out by mechanical force using H...

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Main Authors:	Ahmad Raus, Raha, Mel, Maizirwan, Mohd Abdullah, S. N., Yusoff, K.
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/1251/ http://irep.iium.edu.my/1251/ http://irep.iium.edu.my/1251/ http://irep.iium.edu.my/1251/1/Cell_Rupture_of_Recombinant_E._coli_Using_High_Pressure_Homogenizer.pdf

id	iium-1251
recordtype	eprints
spelling	iium-12512012-01-16T08:10:32Z http://irep.iium.edu.my/1251/ Cell rupture of recombinant escherichia coli using high pressure homogenizer Ahmad Raus, Raha Mel, Maizirwan Mohd Abdullah, S. N. Yusoff, K. TP248.13 Biotechnology Cell rupture is one of the earlier steps in downstream processing which are required for the recovery of biological products that are located inside cells. Cells could be disrupted either by using chemicals or mechanical method. In this study, cell rupture was carried out by mechanical force using High Pressure Homogenizer (HPH). The aim of this study is to identify optimal conditions of HPH to disrupt the cell wall of recombinant Escherichia coli harboring nucleocapsid (NP) gene of Newcastle Disease Virus (NDV). The optimized conditions were achieved by manipulating the independent variables of HPH such as pressure, pump speed and number of cycles through an optimization process. The efficiency of the cell disruption was determined by estimating the percentage of cell rupture as well as the amount of NP protein released from the cell lysis. Through the means plot analysis of Minitab Software (Version 14.12), pressure was recognized as the main factor for achieving the highest cell rupture and the release of NP protein. The optimized conditions for obtaining the highest NP protein yield were by operating three cycles of cell rupture, homogenizer pressure of 800 bars and pump speed of 7 psi. Asian Network for Scientific Information 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/1251/1/Cell_Rupture_of_Recombinant_E._coli_Using_High_Pressure_Homogenizer.pdf Ahmad Raus, Raha and Mel, Maizirwan and Mohd Abdullah, S. N. and Yusoff, K. (2010) Cell rupture of recombinant escherichia coli using high pressure homogenizer. Journal of Applied Sciences, 10 (21). pp. 2717-2720. ISSN 1812-5662 (O), 1812-5654 (P) http://scialert.net/abstract/?doi=jas.2010.2717.2720 10.3923/jas.2010.2717.2720
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ahmad Raus, Raha Mel, Maizirwan Mohd Abdullah, S. N. Yusoff, K. Cell rupture of recombinant escherichia coli using high pressure homogenizer
description	Cell rupture is one of the earlier steps in downstream processing which are required for the recovery of biological products that are located inside cells. Cells could be disrupted either by using chemicals or mechanical method. In this study, cell rupture was carried out by mechanical force using High Pressure Homogenizer (HPH). The aim of this study is to identify optimal conditions of HPH to disrupt the cell wall of recombinant Escherichia coli harboring nucleocapsid (NP) gene of Newcastle Disease Virus (NDV). The optimized conditions were achieved by manipulating the independent variables of HPH such as pressure, pump speed and number of cycles through an optimization process. The efficiency of the cell disruption was determined by estimating the percentage of cell rupture as well as the amount of NP protein released from the cell lysis. Through the means plot analysis of Minitab Software (Version 14.12), pressure was recognized as the main factor for achieving the highest cell rupture and the release of NP protein. The optimized conditions for obtaining the highest NP protein yield were by operating three cycles of cell rupture, homogenizer pressure of 800 bars and pump speed of 7 psi.
format	Article
author	Ahmad Raus, Raha Mel, Maizirwan Mohd Abdullah, S. N. Yusoff, K.
author_facet	Ahmad Raus, Raha Mel, Maizirwan Mohd Abdullah, S. N. Yusoff, K.
author_sort	Ahmad Raus, Raha
title	Cell rupture of recombinant escherichia coli using high pressure homogenizer
title_short	Cell rupture of recombinant escherichia coli using high pressure homogenizer
title_full	Cell rupture of recombinant escherichia coli using high pressure homogenizer
title_fullStr	Cell rupture of recombinant escherichia coli using high pressure homogenizer
title_full_unstemmed	Cell rupture of recombinant escherichia coli using high pressure homogenizer
title_sort	cell rupture of recombinant escherichia coli using high pressure homogenizer
publisher	Asian Network for Scientific Information
publishDate	2010
url	http://irep.iium.edu.my/1251/ http://irep.iium.edu.my/1251/ http://irep.iium.edu.my/1251/ http://irep.iium.edu.my/1251/1/Cell_Rupture_of_Recombinant_E._coli_Using_High_Pressure_Homogenizer.pdf
first_indexed	2023-09-18T20:08:29Z
last_indexed	2023-09-18T20:08:29Z
_version_	1777407318073278464

Cell rupture of recombinant escherichia coli using high pressure homogenizer

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