Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer

The potential of Streptomyces sp. UKMCC-PT15 that was isolated from the gut of a sea cucumber from Pulau Tinggi, Johor as an anticancer agent producer was investigated. The isolate was able to tolerate up to 12% salt concentration and temperature of up to 45oC. Both polyketide synthase type I (PKS-I...

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Main Authors:	Zainal Abidin, Zaima Azira, Usup, Gires, Ahmad, Asmat, Lu, Chung-Kuang
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/12733/ http://irep.iium.edu.my/12733/1/poster_penang.pdf

id	iium-12733
recordtype	eprints
spelling	iium-127332012-01-12T08:30:47Z http://irep.iium.edu.my/12733/ Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer Zainal Abidin, Zaima Azira Usup, Gires Ahmad, Asmat Lu, Chung-Kuang Q Science (General) The potential of Streptomyces sp. UKMCC-PT15 that was isolated from the gut of a sea cucumber from Pulau Tinggi, Johor as an anticancer agent producer was investigated. The isolate was able to tolerate up to 12% salt concentration and temperature of up to 45oC. Both polyketide synthase type I (PKS-I) and non-ribosomal peptide syntethases (NRPS) genes that are important in the biosyntheisi of secondary metabolites were present in this isolate. Molecular identification was also performed using 16S rDNA partial sequencing. A crude methanolic extract of this isolate was fractionated using flash column chromatography producing 10 fractions (ZAI1-20-1 – ZAI1-20-10). Fraction 4 (ZAI1-20-4) was then chosen for a further separation step using Sephadex LH-20. A total of 4 fractions were obtained (ZAI1-23-2 –ZAI1-23-4). Cytotoxicity test results against DLD-1 and CCRF-CEM cell lines showed that 5 fractions namely ZAI1-20-1, ZAI1-20-5, ZAI1-20-6, ZAI1-23-2 AND ZAI1-23-4 have the anticancer properties. Fraction 2 (ZAI1-23-2) was selected for further separation using HPLC using a photodiode array detector in the wavelength range 200-800nm on a C18, 5um column. A mobile phase comprising methanol:0.1% acetic acid (95:5) with a flowrate of 1ml/min was the best condition to separate ZAI1-23-2 to 5 fractions (ZAI1-29-1 – ZAI1-29-5). All the fractions were subjected to NMR and it was found that ZAI1-29-1 was successfully purified using HPLC. 2009-12-01 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/12733/1/poster_penang.pdf Zainal Abidin, Zaima Azira and Usup, Gires and Ahmad, Asmat and Lu, Chung-Kuang (2009) Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer. In: International Congress of Malaysian Society for Microbiology, 1-4 December 2009, Penang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Zainal Abidin, Zaima Azira Usup, Gires Ahmad, Asmat Lu, Chung-Kuang Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer
description	The potential of Streptomyces sp. UKMCC-PT15 that was isolated from the gut of a sea cucumber from Pulau Tinggi, Johor as an anticancer agent producer was investigated. The isolate was able to tolerate up to 12% salt concentration and temperature of up to 45oC. Both polyketide synthase type I (PKS-I) and non-ribosomal peptide syntethases (NRPS) genes that are important in the biosyntheisi of secondary metabolites were present in this isolate. Molecular identification was also performed using 16S rDNA partial sequencing. A crude methanolic extract of this isolate was fractionated using flash column chromatography producing 10 fractions (ZAI1-20-1 – ZAI1-20-10). Fraction 4 (ZAI1-20-4) was then chosen for a further separation step using Sephadex LH-20. A total of 4 fractions were obtained (ZAI1-23-2 –ZAI1-23-4). Cytotoxicity test results against DLD-1 and CCRF-CEM cell lines showed that 5 fractions namely ZAI1-20-1, ZAI1-20-5, ZAI1-20-6, ZAI1-23-2 AND ZAI1-23-4 have the anticancer properties. Fraction 2 (ZAI1-23-2) was selected for further separation using HPLC using a photodiode array detector in the wavelength range 200-800nm on a C18, 5um column. A mobile phase comprising methanol:0.1% acetic acid (95:5) with a flowrate of 1ml/min was the best condition to separate ZAI1-23-2 to 5 fractions (ZAI1-29-1 – ZAI1-29-5). All the fractions were subjected to NMR and it was found that ZAI1-29-1 was successfully purified using HPLC.
format	Conference or Workshop Item
author	Zainal Abidin, Zaima Azira Usup, Gires Ahmad, Asmat Lu, Chung-Kuang
author_facet	Zainal Abidin, Zaima Azira Usup, Gires Ahmad, Asmat Lu, Chung-Kuang
author_sort	Zainal Abidin, Zaima Azira
title	Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer
title_short	Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer
title_full	Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer
title_fullStr	Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer
title_full_unstemmed	Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer
title_sort	streptomyces sp. ukmcc_pt15: potential anticancer agent producer
publishDate	2009
url	http://irep.iium.edu.my/12733/ http://irep.iium.edu.my/12733/1/poster_penang.pdf
first_indexed	2023-09-18T20:21:53Z
last_indexed	2023-09-18T20:21:53Z
_version_	1777408162136064000

Streptomyces sp. UKMCC_PT15: Potential anticancer agent producer

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