Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint

ABSTRACT The aim of this study to determine if intra-articular injection of autologous bone marrow mesenchymal stem cells (ABMSCs) could repair surgically induced osteoarthritis in sheep model. Eighteen male healthy sheep (weighed 18-20kg) were divided into two test groups and one control grou...

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Main Authors:	Alfaqeh, Hamoud Hussein Mohammed, Mohamad Yahaya, Norhamdan, Chen, Hui Cheng, Chua, Kienhui, Saim, Aminuddin, Haji Idrus, Ruszymah
Format:	Conference or Workshop Item
Language:	English English English
Published:	2010
Subjects:	QP Physiology QR Microbiology
Online Access:	http://irep.iium.edu.my/12747/ http://irep.iium.edu.my/12747/ http://irep.iium.edu.my/12747/1/Abstract_for_TERMIS2010_-Sydney.pdf http://irep.iium.edu.my/12747/4/termisAP2010_abstracts.pdf http://irep.iium.edu.my/12747/7/Stem_cell_based_therapy.pdf

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spelling	iium-127472015-12-04T08:47:52Z http://irep.iium.edu.my/12747/ Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint Alfaqeh, Hamoud Hussein Mohammed Mohamad Yahaya, Norhamdan Chen, Hui Cheng Chua, Kienhui Saim, Aminuddin Haji Idrus, Ruszymah QP Physiology QR Microbiology ABSTRACT The aim of this study to determine if intra-articular injection of autologous bone marrow mesenchymal stem cells (ABMSCs) could repair surgically induced osteoarthritis in sheep model. Eighteen male healthy sheep (weighed 18-20kg) were divided into two test groups and one control group. The control groups were not different from the test groups with respect to age and weight, but the test animals underwent a bone marrow aspira- tion for cell preparation in the same time of osteoarthritis induction. ABMSCs were isolated from sheep bone marrow and divided into two groups, namely test group A; ABMSCs cultured in FD medium supplemented with 10% fetal bovine serum (FBS), Test group B; ABMSCs were cultured in FD medium supplimented with 1% FBS and 10 ng/ml TGFβ-3 for three weeks. OA was induced by complete excision of the medial meniscus and resection of the anterior cruciate ligament (ACL). Sheep were subjected to exercise for three weeks post OA induction. After 6 weeks post-operation, test groups received direct intra-articular injection of a single dose 10x106 cells suspended in basal medium into injured sheep knee joint. Con- trol animals received basal medium alone. Six weeks post- cel injection, the femoral condyle and the tibial plateau from test and control groups were removed,fixed,photographed, and assessed by two blinded evaluators based on ICRS grading system, decalcified. Specimens were sectioned into 5 µm and stained with H & E and Safranin O. The result demonstrated that Gross observation of femoral condyle and tibia plaetue of the operated knee joint had OA. The severe OA was clearly observed in in control group knee joints. Test group received intra-articular injection of ABMSCs alone showed moderate OA. Interestingly test group B that received intra-articularinjection of TGF-β3 induced ABMSCs showed mild OA. The histological examination showed clear evidence of articular cartilage and menicscus regeneration in test group B of sheep injured knee joint that received TGF-β3 induced ABMSCs when compared with other groups. 2010-09-16 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/12747/1/Abstract_for_TERMIS2010_-Sydney.pdf application/pdf en http://irep.iium.edu.my/12747/4/termisAP2010_abstracts.pdf application/pdf en http://irep.iium.edu.my/12747/7/Stem_cell_based_therapy.pdf Alfaqeh, Hamoud Hussein Mohammed and Mohamad Yahaya, Norhamdan and Chen, Hui Cheng and Chua, Kienhui and Saim, Aminuddin and Haji Idrus, Ruszymah (2010) Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint. In: Tissue Engineering & Regenerative Medicine International Society 2010 Asia Pacific meeting 15 -17 September 2010. Sheraton on the Park, Sydney, NSW, Australia. , 15-17 Sept 2010, Australia-Sydney. http://www.termis.org/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	QP Physiology QR Microbiology
spellingShingle	QP Physiology QR Microbiology Alfaqeh, Hamoud Hussein Mohammed Mohamad Yahaya, Norhamdan Chen, Hui Cheng Chua, Kienhui Saim, Aminuddin Haji Idrus, Ruszymah Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint
description	ABSTRACT The aim of this study to determine if intra-articular injection of autologous bone marrow mesenchymal stem cells (ABMSCs) could repair surgically induced osteoarthritis in sheep model. Eighteen male healthy sheep (weighed 18-20kg) were divided into two test groups and one control group. The control groups were not different from the test groups with respect to age and weight, but the test animals underwent a bone marrow aspira- tion for cell preparation in the same time of osteoarthritis induction. ABMSCs were isolated from sheep bone marrow and divided into two groups, namely test group A; ABMSCs cultured in FD medium supplemented with 10% fetal bovine serum (FBS), Test group B; ABMSCs were cultured in FD medium supplimented with 1% FBS and 10 ng/ml TGFβ-3 for three weeks. OA was induced by complete excision of the medial meniscus and resection of the anterior cruciate ligament (ACL). Sheep were subjected to exercise for three weeks post OA induction. After 6 weeks post-operation, test groups received direct intra-articular injection of a single dose 10x106 cells suspended in basal medium into injured sheep knee joint. Con- trol animals received basal medium alone. Six weeks post- cel injection, the femoral condyle and the tibial plateau from test and control groups were removed,fixed,photographed, and assessed by two blinded evaluators based on ICRS grading system, decalcified. Specimens were sectioned into 5 µm and stained with H & E and Safranin O. The result demonstrated that Gross observation of femoral condyle and tibia plaetue of the operated knee joint had OA. The severe OA was clearly observed in in control group knee joints. Test group received intra-articular injection of ABMSCs alone showed moderate OA. Interestingly test group B that received intra-articularinjection of TGF-β3 induced ABMSCs showed mild OA. The histological examination showed clear evidence of articular cartilage and menicscus regeneration in test group B of sheep injured knee joint that received TGF-β3 induced ABMSCs when compared with other groups.
format	Conference or Workshop Item
author	Alfaqeh, Hamoud Hussein Mohammed Mohamad Yahaya, Norhamdan Chen, Hui Cheng Chua, Kienhui Saim, Aminuddin Haji Idrus, Ruszymah
author_facet	Alfaqeh, Hamoud Hussein Mohammed Mohamad Yahaya, Norhamdan Chen, Hui Cheng Chua, Kienhui Saim, Aminuddin Haji Idrus, Ruszymah
author_sort	Alfaqeh, Hamoud Hussein Mohammed
title	Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint
title_short	Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint
title_full	Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint
title_fullStr	Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint
title_full_unstemmed	Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint
title_sort	stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint
publishDate	2010
url	http://irep.iium.edu.my/12747/ http://irep.iium.edu.my/12747/ http://irep.iium.edu.my/12747/1/Abstract_for_TERMIS2010_-Sydney.pdf http://irep.iium.edu.my/12747/4/termisAP2010_abstracts.pdf http://irep.iium.edu.my/12747/7/Stem_cell_based_therapy.pdf
first_indexed	2023-09-18T20:21:54Z
last_indexed	2023-09-18T20:21:54Z
_version_	1777408162883698688

Stem cell based therapy retards the progression of osteoarthritis and promotes repair of meniscus injury of sheep model knee joint

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