Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology

The aim of this study is to determine the antioxidant properties of methanolic and ethanolic extracts of four Malaysian aromatic plants’ leaves, namely knotweed (Polygonum minus), curry (Murraya koenigii), kaffir lime (Citrus hysrix) and fragrant screwpine (Pandanus odurus) as a head start for indus...

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Main Authors:	Kamarudin, Kamarul Rahim, Abu Abas, Azlim Almey, Koya, Mustapha Suleiman, Syed Osman Idid, Syed Zahir Idid, Mohamed Rehan, Aisyah, Khan Chowdhury, Ahmed Jalal
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/12960/ http://irep.iium.edu.my/12960/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf

id	iium-12960
recordtype	eprints
spelling	iium-129602017-12-06T04:54:13Z http://irep.iium.edu.my/12960/ Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology Kamarudin, Kamarul Rahim Abu Abas, Azlim Almey Koya, Mustapha Suleiman Syed Osman Idid, Syed Zahir Idid Mohamed Rehan, Aisyah Khan Chowdhury, Ahmed Jalal Q Science (General) The aim of this study is to determine the antioxidant properties of methanolic and ethanolic extracts of four Malaysian aromatic plants’ leaves, namely knotweed (Polygonum minus), curry (Murraya koenigii), kaffir lime (Citrus hysrix) and fragrant screwpine (Pandanus odurus) as a head start for industrial biotechnology. Total phenolic content (TPC) assay using Folin-Ciocalteu method was used to assess the presence and level of phenolic compounds in each sample. Antioxidant activity in terms of total antioxidant and free radical scavenging activities of both methanolic and ethanolic extracts was measured by β-carotene bleaching assay and 2, 2, diphenyl-1- icrylhydrazyl (DPPH) free radical scavenging activity assay, respectively. The present results showed that methanolic extract of P. minus exhibited the highest total antioxidant activity (98.2 ±0.5%) while for the EC50 based on DPPH. radical scavenging activity assay, the lowest concentration was shown by P. minus extracts as compared to the other samples. For both ethanolic and methanolic extracts, moderately strong and strong positive correlations existed between the phenolic content and antioxidant activity based on β-carotene bleaching assay, respectively. Among the eluent systems used for phytochemical analysis using thin layer chromatography (TLC), EWFA showed the largest number of antioxidant active compounds, followed by EMW and CEF. HEA eluent system indicated no presence of antioxidant active compound showing that most compounds were polar. Retardation factor (Rf) values were calculated between 0.2 to 0.6. Relatively, the present results suggested that of the four aromatic plants, P. minus and M. koenigii have high potential as sources of commercial natural antioxidants regardless types of extracting solvents. 2010-01-26 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/12960/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf Kamarudin, Kamarul Rahim and Abu Abas, Azlim Almey and Koya, Mustapha Suleiman and Syed Osman Idid, Syed Zahir Idid and Mohamed Rehan, Aisyah and Khan Chowdhury, Ahmed Jalal (2010) Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Kamarudin, Kamarul Rahim Abu Abas, Azlim Almey Koya, Mustapha Suleiman Syed Osman Idid, Syed Zahir Idid Mohamed Rehan, Aisyah Khan Chowdhury, Ahmed Jalal Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology
description	The aim of this study is to determine the antioxidant properties of methanolic and ethanolic extracts of four Malaysian aromatic plants’ leaves, namely knotweed (Polygonum minus), curry (Murraya koenigii), kaffir lime (Citrus hysrix) and fragrant screwpine (Pandanus odurus) as a head start for industrial biotechnology. Total phenolic content (TPC) assay using Folin-Ciocalteu method was used to assess the presence and level of phenolic compounds in each sample. Antioxidant activity in terms of total antioxidant and free radical scavenging activities of both methanolic and ethanolic extracts was measured by β-carotene bleaching assay and 2, 2, diphenyl-1- icrylhydrazyl (DPPH) free radical scavenging activity assay, respectively. The present results showed that methanolic extract of P. minus exhibited the highest total antioxidant activity (98.2 ±0.5%) while for the EC50 based on DPPH. radical scavenging activity assay, the lowest concentration was shown by P. minus extracts as compared to the other samples. For both ethanolic and methanolic extracts, moderately strong and strong positive correlations existed between the phenolic content and antioxidant activity based on β-carotene bleaching assay, respectively. Among the eluent systems used for phytochemical analysis using thin layer chromatography (TLC), EWFA showed the largest number of antioxidant active compounds, followed by EMW and CEF. HEA eluent system indicated no presence of antioxidant active compound showing that most compounds were polar. Retardation factor (Rf) values were calculated between 0.2 to 0.6. Relatively, the present results suggested that of the four aromatic plants, P. minus and M. koenigii have high potential as sources of commercial natural antioxidants regardless types of extracting solvents.
format	Conference or Workshop Item
author	Kamarudin, Kamarul Rahim Abu Abas, Azlim Almey Koya, Mustapha Suleiman Syed Osman Idid, Syed Zahir Idid Mohamed Rehan, Aisyah Khan Chowdhury, Ahmed Jalal
author_facet	Kamarudin, Kamarul Rahim Abu Abas, Azlim Almey Koya, Mustapha Suleiman Syed Osman Idid, Syed Zahir Idid Mohamed Rehan, Aisyah Khan Chowdhury, Ahmed Jalal
author_sort	Kamarudin, Kamarul Rahim
title	Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology
title_short	Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology
title_full	Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology
title_fullStr	Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology
title_full_unstemmed	Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology
title_sort	antioxidant study of methanolic and ethanolic extracts of malaysian aromatic plants’ leaves: towards industrial biotechnology
publishDate	2010
url	http://irep.iium.edu.my/12960/ http://irep.iium.edu.my/12960/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf
first_indexed	2023-09-18T20:22:05Z
last_indexed	2023-09-18T20:22:05Z
_version_	1777408174340440064

Antioxidant study of methanolic and ethanolic extracts of Malaysian aromatic plants’ leaves: towards industrial biotechnology

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