Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor
The production of monoclonal antibody (MAbs) for anti-cancer by fermentation of hybridoma cell in bench-top bioreactor was investigated. The main aim of this study was to increase the percentage of cell viability and to achieve high yield of IgM. Many ways can be applied to improve the process and...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/13646/ http://irep.iium.edu.my/13646/ http://irep.iium.edu.my/13646/ http://irep.iium.edu.my/13646/1/Mel_et_al.pdf |
| id |
iium-13646 |
|---|---|
| recordtype |
eprints |
| spelling |
iium-136462012-07-06T02:43:01Z http://irep.iium.edu.my/13646/ Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor Mel, Maizirwan Arifin, Mohd Azmir Kamaruddin, Mohd Hider Abdul Karim, Mohamed Ismail Yusof, Faridah TP155 Chemical engineering TP248.13 Biotechnology The production of monoclonal antibody (MAbs) for anti-cancer by fermentation of hybridoma cell in bench-top bioreactor was investigated. The main aim of this study was to increase the percentage of cell viability and to achieve high yield of IgM. Many ways can be applied to improve the process and one of them is by choosing the correct six sigma’s design of experiment (DOE). In this study, Taguchi’s experimental design was applied to achieve high percentage of cell viability in the fermentation experiment. The process capability of this study was later analyzed by using the MiniTab Software (Ver. 14). Analysis showed that this study fulfilled a minimum target of process improvement as indicated by the Cp and Cpk value of 0.90 and 0.86. This proved that the fermentation experiment has achieved cell viability of more than 80% throughout the study. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/13646/1/Mel_et_al.pdf Mel, Maizirwan and Arifin, Mohd Azmir and Kamaruddin, Mohd Hider and Abdul Karim, Mohamed Ismail and Yusof, Faridah (2011) Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor. African Journal of Biotechnology, 10 (81). pp. 18824-18828. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Mel%20et%20al.pdf DOI: 10.5897/AJB11.2760 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
TP155 Chemical engineering TP248.13 Biotechnology |
| spellingShingle |
TP155 Chemical engineering TP248.13 Biotechnology Mel, Maizirwan Arifin, Mohd Azmir Kamaruddin, Mohd Hider Abdul Karim, Mohamed Ismail Yusof, Faridah Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor |
| description |
The production of monoclonal antibody (MAbs) for anti-cancer by fermentation of hybridoma cell in
bench-top bioreactor was investigated. The main aim of this study was to increase the percentage of
cell viability and to achieve high yield of IgM. Many ways can be applied to improve the process and
one of them is by choosing the correct six sigma’s design of experiment (DOE). In this study, Taguchi’s
experimental design was applied to achieve high percentage of cell viability in the fermentation
experiment. The process capability of this study was later analyzed by using the MiniTab Software (Ver.
14). Analysis showed that this study fulfilled a minimum target of process improvement as indicated by
the Cp and Cpk value of 0.90 and 0.86. This proved that the fermentation experiment has achieved cell
viability of more than 80% throughout the study. |
| format |
Article |
| author |
Mel, Maizirwan Arifin, Mohd Azmir Kamaruddin, Mohd Hider Abdul Karim, Mohamed Ismail Yusof, Faridah |
| author_facet |
Mel, Maizirwan Arifin, Mohd Azmir Kamaruddin, Mohd Hider Abdul Karim, Mohamed Ismail Yusof, Faridah |
| author_sort |
Mel, Maizirwan |
| title |
Simple process capability analysis and quality
validation of monoclonal antibody production in bench-top
bioreactor |
| title_short |
Simple process capability analysis and quality
validation of monoclonal antibody production in bench-top
bioreactor |
| title_full |
Simple process capability analysis and quality
validation of monoclonal antibody production in bench-top
bioreactor |
| title_fullStr |
Simple process capability analysis and quality
validation of monoclonal antibody production in bench-top
bioreactor |
| title_full_unstemmed |
Simple process capability analysis and quality
validation of monoclonal antibody production in bench-top
bioreactor |
| title_sort |
simple process capability analysis and quality
validation of monoclonal antibody production in bench-top
bioreactor |
| publisher |
Academic Journals |
| publishDate |
2011 |
| url |
http://irep.iium.edu.my/13646/ http://irep.iium.edu.my/13646/ http://irep.iium.edu.my/13646/ http://irep.iium.edu.my/13646/1/Mel_et_al.pdf |
| first_indexed |
2023-09-18T20:22:47Z |
| last_indexed |
2023-09-18T20:22:47Z |
| _version_ |
1777408218001047552 |