Optimization of Newcastle disease virus production in T-flask

Newcastle disease (ND) is regarded as one of the most important diseases in the poultry industry. Currently, vaccines for ND are produced by using embryonated chicken eggs, a method which has the disadvantages of being labour-intensive, time consuming and requires large area for the incubation of...

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Main Authors:	Arifin, Mohd Azmir, Mel, Maizirwan, Salim, Siti Hajar, Abdul Karim, Mohd Ismail, Hassan, Sharifah Syed
Format:	Article
Language:	English
Published:	Academic Journals 2011
Subjects:	TP155 Chemical engineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/13687/ http://irep.iium.edu.my/13687/ http://irep.iium.edu.my/13687/ http://irep.iium.edu.my/13687/1/Arifin_et_al.pdf

id	iium-13687
recordtype	eprints
spelling	iium-136872012-01-04T06:56:10Z http://irep.iium.edu.my/13687/ Optimization of Newcastle disease virus production in T-flask Arifin, Mohd Azmir Mel, Maizirwan Salim, Siti Hajar Abdul Karim, Mohd Ismail Hassan, Sharifah Syed TP155 Chemical engineering TP248.13 Biotechnology Newcastle disease (ND) is regarded as one of the most important diseases in the poultry industry. Currently, vaccines for ND are produced by using embryonated chicken eggs, a method which has the disadvantages of being labour-intensive, time consuming and requires large area for the incubation of eggs. In the present study, the production of lentogenic Asplin F strain of Newcastle disease virus by using cell culture method was studied. Experiments were carried out in T-flasks to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on ND virus propagation in DF-1 cells. Results show that virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/13687/1/Arifin_et_al.pdf Arifin, Mohd Azmir and Mel, Maizirwan and Salim, Siti Hajar and Abdul Karim, Mohd Ismail and Hassan, Sharifah Syed (2011) Optimization of Newcastle disease virus production in T-flask. African Journal of Biotechnology, 10 (81). pp. 18816-18823. ISSN 1684–5315 http://www.academicjournals.org/AJB/abstracts/abs2011/16DecConf/Arifin%20et%20al.htm DOI: 10.5897/AJB11.2759
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering TP248.13 Biotechnology
spellingShingle	TP155 Chemical engineering TP248.13 Biotechnology Arifin, Mohd Azmir Mel, Maizirwan Salim, Siti Hajar Abdul Karim, Mohd Ismail Hassan, Sharifah Syed Optimization of Newcastle disease virus production in T-flask
description	Newcastle disease (ND) is regarded as one of the most important diseases in the poultry industry. Currently, vaccines for ND are produced by using embryonated chicken eggs, a method which has the disadvantages of being labour-intensive, time consuming and requires large area for the incubation of eggs. In the present study, the production of lentogenic Asplin F strain of Newcastle disease virus by using cell culture method was studied. Experiments were carried out in T-flasks to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on ND virus propagation in DF-1 cells. Results show that virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used
format	Article
author	Arifin, Mohd Azmir Mel, Maizirwan Salim, Siti Hajar Abdul Karim, Mohd Ismail Hassan, Sharifah Syed
author_facet	Arifin, Mohd Azmir Mel, Maizirwan Salim, Siti Hajar Abdul Karim, Mohd Ismail Hassan, Sharifah Syed
author_sort	Arifin, Mohd Azmir
title	Optimization of Newcastle disease virus production in T-flask
title_short	Optimization of Newcastle disease virus production in T-flask
title_full	Optimization of Newcastle disease virus production in T-flask
title_fullStr	Optimization of Newcastle disease virus production in T-flask
title_full_unstemmed	Optimization of Newcastle disease virus production in T-flask
title_sort	optimization of newcastle disease virus production in t-flask
publisher	Academic Journals
publishDate	2011
url	http://irep.iium.edu.my/13687/ http://irep.iium.edu.my/13687/ http://irep.iium.edu.my/13687/ http://irep.iium.edu.my/13687/1/Arifin_et_al.pdf
first_indexed	2023-09-18T20:22:48Z
last_indexed	2023-09-18T20:22:48Z
_version_	1777408219322253312

Optimization of Newcastle disease virus production in T-flask

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