Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology

Ethanol production by co-culture of ragi tapai and Saccharomyces cerevisiae from unhydrolyzed cassava starch without addition of enzymes was conducted in a 2 L batch fermentor. Taguchi’s method with orthogonal array of L8 was applied in design of experiment (DOE) and the results were analyzed usi...

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Main Authors: Azmi, Azlin Suhaida, Ngoh, Cheng G., Mel, Maizirwan
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
Online Access:http://irep.iium.edu.my/13696/
http://irep.iium.edu.my/13696/
http://irep.iium.edu.my/13696/
http://irep.iium.edu.my/13696/1/Azlen_Suhaida_AJB.pdf
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spelling iium-136962012-11-04T08:40:27Z http://irep.iium.edu.my/13696/ Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology Azmi, Azlin Suhaida Ngoh, Cheng G. Mel, Maizirwan TP155 Chemical engineering TP248.13 Biotechnology Ethanol production by co-culture of ragi tapai and Saccharomyces cerevisiae from unhydrolyzed cassava starch without addition of enzymes was conducted in a 2 L batch fermentor. Taguchi’s method with orthogonal array of L8 was applied in design of experiment (DOE) and the results were analyzed using MINITAB v14 software. Seven factors: Nitrogen-phosphorus-potassium (NPK), urea, fermentation temperature, ragi tapai concentration, S. cerevisiae concentration, agitation and co-culturing time were varied at two levels for each factor. The significant factors for the production of ethanol were determined by setting S/N ratio to “larger-is-better” for high yield ethanol and “smaller-is-better” for low yield of ethanol byproducts. The optimum values obtained for each factor were similar to each other. Both have optimum factors of: Urea at 0.8 w/w%, dry ragi tapai and S. cerevisiae concentrations each at 10 w/w%, co-culturing time at 3 h gap, NPK at 0.09 w/w% and agitation speed at 100 rpm. The fermentation temperature for high ethanol yield was 35°C, whereas for the byproducts was 30°C. From the validation experiment conducted at 30°C in 10 L fermentor, the ethanol concentration obtained was 68.00 g/L, while all byproducts concentrations were below 9.00 g/L at the end of the fermentation. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/13696/1/Azlen_Suhaida_AJB.pdf Azmi, Azlin Suhaida and Ngoh, Cheng G. and Mel, Maizirwan (2011) Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology. African Journal of Biotechnology, 10 (81). pp. 18833-18841. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Azmi%20et%20al.pdf 10.5897/AJB11.2762
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP155 Chemical engineering
TP248.13 Biotechnology
spellingShingle TP155 Chemical engineering
TP248.13 Biotechnology
Azmi, Azlin Suhaida
Ngoh, Cheng G.
Mel, Maizirwan
Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology
description Ethanol production by co-culture of ragi tapai and Saccharomyces cerevisiae from unhydrolyzed cassava starch without addition of enzymes was conducted in a 2 L batch fermentor. Taguchi’s method with orthogonal array of L8 was applied in design of experiment (DOE) and the results were analyzed using MINITAB v14 software. Seven factors: Nitrogen-phosphorus-potassium (NPK), urea, fermentation temperature, ragi tapai concentration, S. cerevisiae concentration, agitation and co-culturing time were varied at two levels for each factor. The significant factors for the production of ethanol were determined by setting S/N ratio to “larger-is-better” for high yield ethanol and “smaller-is-better” for low yield of ethanol byproducts. The optimum values obtained for each factor were similar to each other. Both have optimum factors of: Urea at 0.8 w/w%, dry ragi tapai and S. cerevisiae concentrations each at 10 w/w%, co-culturing time at 3 h gap, NPK at 0.09 w/w% and agitation speed at 100 rpm. The fermentation temperature for high ethanol yield was 35°C, whereas for the byproducts was 30°C. From the validation experiment conducted at 30°C in 10 L fermentor, the ethanol concentration obtained was 68.00 g/L, while all byproducts concentrations were below 9.00 g/L at the end of the fermentation.
format Article
author Azmi, Azlin Suhaida
Ngoh, Cheng G.
Mel, Maizirwan
author_facet Azmi, Azlin Suhaida
Ngoh, Cheng G.
Mel, Maizirwan
author_sort Azmi, Azlin Suhaida
title Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology
title_short Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology
title_full Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology
title_fullStr Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology
title_full_unstemmed Prediction of significant factors in the production of ethanol by ragi tapai co-culture using Taguchi methodology
title_sort prediction of significant factors in the production of ethanol by ragi tapai co-culture using taguchi methodology
publisher Academic Journals
publishDate 2011
url http://irep.iium.edu.my/13696/
http://irep.iium.edu.my/13696/
http://irep.iium.edu.my/13696/
http://irep.iium.edu.my/13696/1/Azlen_Suhaida_AJB.pdf
first_indexed 2023-09-18T20:22:49Z
last_indexed 2023-09-18T20:22:49Z
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