Optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media

A research was conducted on liquid state bioconversion for the production of citric acid using sewage treatment plant (STP) sludge as the main substrate with supplementation of sucrose as co-substrate. Three parameters were examined to determine the best process optimization conducted in two liter (...

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Main Authors: Mohamad Hanapi, Nur Hanis, Alam, Md Zahangir, Abdul Karim, Mohamed Ismail
Format: Article
Language:English
Published: Academic Journals 2011
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Online Access:http://irep.iium.edu.my/14264/
http://irep.iium.edu.my/14264/
http://irep.iium.edu.my/14264/
http://irep.iium.edu.my/14264/1/Hanapi_et_al_%281%29.pdf
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spelling iium-142642017-08-10T02:35:30Z http://irep.iium.edu.my/14264/ Optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media Mohamad Hanapi, Nur Hanis Alam, Md Zahangir Abdul Karim, Mohamed Ismail QR Microbiology A research was conducted on liquid state bioconversion for the production of citric acid using sewage treatment plant (STP) sludge as the main substrate with supplementation of sucrose as co-substrate. Three parameters were examined to determine the best process optimization conducted in two liter (2L) bioreactor. Two level factorial designs with three parameters were applied for the fermentation. It was carried out using local isolates of Aspergillus niger strain O103A at room temperature (30 to 32°C),agitation was set at three different values which are 100, 200 and 300 rpm. Initial pH for fermentation was set at 5 and it was further adjusted to three levels which are 2, 3 and 4 on the second day of the fermentation. Another parameter was aeration where 1, 1.5 and 2 vvm were introduced. The fermentation was carried out for five days. The maximum yield of citric acid obtained throughout the fermentation was 23.68 g/L, which is due to low pH, low agitation and high aeration values. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14264/1/Hanapi_et_al_%281%29.pdf Mohamad Hanapi, Nur Hanis and Alam, Md Zahangir and Abdul Karim, Mohamed Ismail (2011) Optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media. African Journal of Biotechnology, 10 (81). 18807-18810. ISSN ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Hanapi%20et%20al.pdf 10.5897/AJB11.2757
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QR Microbiology
spellingShingle QR Microbiology
Mohamad Hanapi, Nur Hanis
Alam, Md Zahangir
Abdul Karim, Mohamed Ismail
Optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media
description A research was conducted on liquid state bioconversion for the production of citric acid using sewage treatment plant (STP) sludge as the main substrate with supplementation of sucrose as co-substrate. Three parameters were examined to determine the best process optimization conducted in two liter (2L) bioreactor. Two level factorial designs with three parameters were applied for the fermentation. It was carried out using local isolates of Aspergillus niger strain O103A at room temperature (30 to 32°C),agitation was set at three different values which are 100, 200 and 300 rpm. Initial pH for fermentation was set at 5 and it was further adjusted to three levels which are 2, 3 and 4 on the second day of the fermentation. Another parameter was aeration where 1, 1.5 and 2 vvm were introduced. The fermentation was carried out for five days. The maximum yield of citric acid obtained throughout the fermentation was 23.68 g/L, which is due to low pH, low agitation and high aeration values.
format Article
author Mohamad Hanapi, Nur Hanis
Alam, Md Zahangir
Abdul Karim, Mohamed Ismail
author_facet Mohamad Hanapi, Nur Hanis
Alam, Md Zahangir
Abdul Karim, Mohamed Ismail
author_sort Mohamad Hanapi, Nur Hanis
title Optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media
title_short Optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media
title_full Optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media
title_fullStr Optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media
title_full_unstemmed Optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media
title_sort optimization of process parameters for the production of citric acid using sewage sludge as major fermentation media
publisher Academic Journals
publishDate 2011
url http://irep.iium.edu.my/14264/
http://irep.iium.edu.my/14264/
http://irep.iium.edu.my/14264/
http://irep.iium.edu.my/14264/1/Hanapi_et_al_%281%29.pdf
first_indexed 2023-09-18T20:23:26Z
last_indexed 2023-09-18T20:23:26Z
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