Physical ageing and thermal analysis of PLGA microspheres encapsulating protein or DNA
PLGA microspheres undergo physical ageing but their ageing kinetics have not been reported, nor the effect of encapsulated protein or plasmid DNA on any associated changes to the glass transition. Differential scanning calorimetry (DSC) was used to measure the rate of ageing of various PLGAmicrosp...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier Inc.
2007
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/1462/ http://irep.iium.edu.my/1462/ http://irep.iium.edu.my/1462/ http://irep.iium.edu.my/1462/1/physical_ageing_paper_van_der_walle_cf.pdf |
| Summary: | PLGA microspheres undergo physical ageing but their ageing kinetics have not been reported, nor the effect of encapsulated protein or plasmid
DNA on any associated changes to the glass transition. Differential scanning calorimetry (DSC) was used to measure the rate of ageing of various
PLGAmicrosphere formulations, with temperature-modulatedDSCused to accurately measure the associated glass transition. The Cowie–Ferguson
model was applied to determine the parameters describing the enthalpy relaxation kinetics. We show that encapsulated proteins had no significant
effect on the glass transition of the microspheres, whereas DNA and PVA were mild antiplasticising agents, particularly with high Mw PLGA.
Physical ageing occurred through a range of enthalpy relaxation times (or modes) and was independent of both encapsulated protein and surfactant
used during microsphere preparation. Analysis of accelerated ageing at 35 ◦C gave calculated enthalpy relaxation times to thermal equilibrium of
280–400 h. No ageing was observed ≤10 ◦C and at 25 ◦C estimated relaxation times were at least one order of magnitude greater than at 35 ◦C.
Ageing of PLGA microspheres therefore occurs at temperatures >10 ◦C, but relaxation will be far from equilibrium unless storage times and/or
temperatures are prolonged or nearing the glass transition, respectively. |
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