Microencapsulation of Cassia Alata extract into biodegradable PLGA

Senna alata or Cassia alata (C. Alata) is a plant belonging to the family Leguminosae and it has antifungal and antimicrobial activities.This research aims to extract Cassia alata using known extraction procedure, followed by microencapsulation using biodegradable Poly (lactide-co-glycolide) (PLGA)....

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Main Authors:	Alfatama, Mulham, Ahmad, Kausar, Doolaanea, Abd Monem, Ali, Aimen Abdo Elsaid, Ismail, Ahmad Fahmi Harun, Mohamed, Farahidah
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	Q Science (General) RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/15175/ http://irep.iium.edu.my/15175/1/MSPP2011calatawithplga.pdf

id	iium-15175
recordtype	eprints
spelling	iium-151752013-01-14T05:41:25Z http://irep.iium.edu.my/15175/ Microencapsulation of Cassia Alata extract into biodegradable PLGA Alfatama, Mulham Ahmad, Kausar Doolaanea, Abd Monem Ali, Aimen Abdo Elsaid Ismail, Ahmad Fahmi Harun Mohamed, Farahidah Q Science (General) RS Pharmacy and materia medica Senna alata or Cassia alata (C. Alata) is a plant belonging to the family Leguminosae and it has antifungal and antimicrobial activities.This research aims to extract Cassia alata using known extraction procedure, followed by microencapsulation using biodegradable Poly (lactide-co-glycolide) (PLGA). The microencapsulation is envisaged to release the extract in a controlled manner. For microsphere fabrication, aqueous extract of C. alata was added to PLGA solution, previously dissolved in DCM, and was homogenized to form the primary water-in-oil (w/o) emulsion. This w/o emulsion was immediately injected into 1% aqueous PVA and homogenized to form the secondary water-in-oil-in-water (w/o/w) emulsion. The w/o/w emulsion was then transferred to a continuously stirred hardening tank of 1% aqueous PVA and the stirring was continued for 2 hours to allow complete evaporation of DCM. The hardened microspheres were collected by centrifugation, washed and freeze-dried. Resultant microspheres were characterized for size distribution and external morphology by laser sizer diffraction technique and scanning electron microscopy, respectively. Encapsulation efficiency(EE) was also calculated and the C. alata was quantified by UV absorbance. Several parameters have been investigated including several types of surfactants, water to oil ratios and buffer systems. It was found that, most of the surfactants employed resulted in low EE (<10%), however with 3% poly vinyl alcohol (PVA), gave slightly higher EE of 11%, with size distribution of 7-13 µm. The EE (50%) was significantly improved when the hardening tank is buffered to pH 7, with minimal effect on particle size. 2011 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/15175/1/MSPP2011calatawithplga.pdf Alfatama, Mulham and Ahmad, Kausar and Doolaanea, Abd Monem and Ali, Aimen Abdo Elsaid and Ismail, Ahmad Fahmi Harun and Mohamed, Farahidah (2011) Microencapsulation of Cassia Alata extract into biodegradable PLGA. In: Malaysian Pharmacology and Physiology Society Conference 2011, 25-26 May 2011, Universiti Putra Malaysia, Serdang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) RS Pharmacy and materia medica
spellingShingle	Q Science (General) RS Pharmacy and materia medica Alfatama, Mulham Ahmad, Kausar Doolaanea, Abd Monem Ali, Aimen Abdo Elsaid Ismail, Ahmad Fahmi Harun Mohamed, Farahidah Microencapsulation of Cassia Alata extract into biodegradable PLGA
description	Senna alata or Cassia alata (C. Alata) is a plant belonging to the family Leguminosae and it has antifungal and antimicrobial activities.This research aims to extract Cassia alata using known extraction procedure, followed by microencapsulation using biodegradable Poly (lactide-co-glycolide) (PLGA). The microencapsulation is envisaged to release the extract in a controlled manner. For microsphere fabrication, aqueous extract of C. alata was added to PLGA solution, previously dissolved in DCM, and was homogenized to form the primary water-in-oil (w/o) emulsion. This w/o emulsion was immediately injected into 1% aqueous PVA and homogenized to form the secondary water-in-oil-in-water (w/o/w) emulsion. The w/o/w emulsion was then transferred to a continuously stirred hardening tank of 1% aqueous PVA and the stirring was continued for 2 hours to allow complete evaporation of DCM. The hardened microspheres were collected by centrifugation, washed and freeze-dried. Resultant microspheres were characterized for size distribution and external morphology by laser sizer diffraction technique and scanning electron microscopy, respectively. Encapsulation efficiency(EE) was also calculated and the C. alata was quantified by UV absorbance. Several parameters have been investigated including several types of surfactants, water to oil ratios and buffer systems. It was found that, most of the surfactants employed resulted in low EE (<10%), however with 3% poly vinyl alcohol (PVA), gave slightly higher EE of 11%, with size distribution of 7-13 µm. The EE (50%) was significantly improved when the hardening tank is buffered to pH 7, with minimal effect on particle size.
format	Conference or Workshop Item
author	Alfatama, Mulham Ahmad, Kausar Doolaanea, Abd Monem Ali, Aimen Abdo Elsaid Ismail, Ahmad Fahmi Harun Mohamed, Farahidah
author_facet	Alfatama, Mulham Ahmad, Kausar Doolaanea, Abd Monem Ali, Aimen Abdo Elsaid Ismail, Ahmad Fahmi Harun Mohamed, Farahidah
author_sort	Alfatama, Mulham
title	Microencapsulation of Cassia Alata extract into biodegradable PLGA
title_short	Microencapsulation of Cassia Alata extract into biodegradable PLGA
title_full	Microencapsulation of Cassia Alata extract into biodegradable PLGA
title_fullStr	Microencapsulation of Cassia Alata extract into biodegradable PLGA
title_full_unstemmed	Microencapsulation of Cassia Alata extract into biodegradable PLGA
title_sort	microencapsulation of cassia alata extract into biodegradable plga
publishDate	2011
url	http://irep.iium.edu.my/15175/ http://irep.iium.edu.my/15175/1/MSPP2011calatawithplga.pdf
first_indexed	2023-09-18T20:24:12Z
last_indexed	2023-09-18T20:24:12Z
_version_	1777408307868205056

Microencapsulation of Cassia Alata extract into biodegradable PLGA

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