Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes

Lactic acid bacteria (LAB) play an important role in aquaculture to develop microbial control strategies since disease outbreaks are recognized as important constraints to aquaculture production and since the development of antibiotic resistance has become a matter of growing concern. In this study...

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Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Khan Chowdhury, Ahmed Jalal, Azhar, Nur Shazana
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	SH Aquaculture. Fisheries. Angling
Online Access:	http://irep.iium.edu.my/15912/ http://irep.iium.edu.my/15912/ http://irep.iium.edu.my/15912/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf

id	iium-15912
recordtype	eprints
spelling	iium-159122017-12-06T04:34:34Z http://irep.iium.edu.my/15912/ Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Azhar, Nur Shazana SH Aquaculture. Fisheries. Angling Lactic acid bacteria (LAB) play an important role in aquaculture to develop microbial control strategies since disease outbreaks are recognized as important constraints to aquaculture production and since the development of antibiotic resistance has become a matter of growing concern. In this study 50 bacterial isolates from the intestinal tract of Clarias Macrocephalus (African Catfish) were tested for the identification of LAB. Phenotypic characteristic was confirmed by Gram staining and identified by other biochemical test such as catalase reaction, lactose utilization and antimicrobial test. Out of the 50 isolates, 30 were Gram-positive cocci. All 30 isolates have the ability to utilize lactose as part of their metabolism process and showed negative reactions towards catalase test. LABs also produce a variety of compounds with antimicrobial activity, and one of them is termed bacteriocin. Bacteriocins are proteinaceous antimicrobial compounds that exhibit bactericidal activity against species closely related to the producer strain. The main screening method that has been used to screen for the antimicrobial activities of bacteriocin is disc diffusion assay. The bacteriocin demonstrated a broad spectrum antagonistic activity against Salmonella Thyphimurium. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/15912/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf Tengku Abdul Hamid, Tengku Haziyamin and Khan Chowdhury, Ahmed Jalal and Azhar, Nur Shazana (2010) Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. http://www.iium.edu.my/irie/10/info/Programme_Book%20Part_2.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SH Aquaculture. Fisheries. Angling
spellingShingle	SH Aquaculture. Fisheries. Angling Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Azhar, Nur Shazana Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes
description	Lactic acid bacteria (LAB) play an important role in aquaculture to develop microbial control strategies since disease outbreaks are recognized as important constraints to aquaculture production and since the development of antibiotic resistance has become a matter of growing concern. In this study 50 bacterial isolates from the intestinal tract of Clarias Macrocephalus (African Catfish) were tested for the identification of LAB. Phenotypic characteristic was confirmed by Gram staining and identified by other biochemical test such as catalase reaction, lactose utilization and antimicrobial test. Out of the 50 isolates, 30 were Gram-positive cocci. All 30 isolates have the ability to utilize lactose as part of their metabolism process and showed negative reactions towards catalase test. LABs also produce a variety of compounds with antimicrobial activity, and one of them is termed bacteriocin. Bacteriocins are proteinaceous antimicrobial compounds that exhibit bactericidal activity against species closely related to the producer strain. The main screening method that has been used to screen for the antimicrobial activities of bacteriocin is disc diffusion assay. The bacteriocin demonstrated a broad spectrum antagonistic activity against Salmonella Thyphimurium.
format	Conference or Workshop Item
author	Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Azhar, Nur Shazana
author_facet	Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Azhar, Nur Shazana
author_sort	Tengku Abdul Hamid, Tengku Haziyamin
title	Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes
title_short	Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes
title_full	Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes
title_fullStr	Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes
title_full_unstemmed	Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes
title_sort	isolation and characterization of lactic acid bacteria from clarias macrocephalus (african catfish) for potential probiotic purposes
publishDate	2010
url	http://irep.iium.edu.my/15912/ http://irep.iium.edu.my/15912/ http://irep.iium.edu.my/15912/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf
first_indexed	2023-09-18T20:24:49Z
last_indexed	2023-09-18T20:24:49Z
_version_	1777408346243989504

Isolation and characterization of lactic acid bacteria from clarias macrocephalus (African Catfish) for potential probiotic purposes

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