Enhancement of polymerase chain reaction using graphene nano-flakes
The excellent heat transfer properties of nanoparticles have potential applications in various fields including biology during the last two decades. Recently, the use of various nanoparticles in polymerase chain reaction (PCR) resulted in significant enhancement of its efficiency and specificity. I...
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iium-161942012-07-19T22:43:12Z http://irep.iium.edu.my/16194/ Enhancement of polymerase chain reaction using graphene nano-flakes Rasheed, Abdul Khaliq Kafafy, Raed Faris, Waleed Fekry Mohd. Salleh, Hamzah TJ Mechanical engineering and machinery TP248.13 Biotechnology The excellent heat transfer properties of nanoparticles have potential applications in various fields including biology during the last two decades. Recently, the use of various nanoparticles in polymerase chain reaction (PCR) resulted in significant enhancement of its efficiency and specificity. In this research we have demonstrated the effects of a novel material, graphene nano-flakes on PCR. The rationale behind the use of graphene flakes is its unique physical and heat transfer properties. A number of experimental results including the effect of graphene flakes on denaturation of DNA and annealing step will also be discussed. The preliminary results clearly show that enhanced heat transfer effect of nano-flakes augment PCR yield and ultimately overall enhancement in reaction efficiency. 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/16194/1/Enhancement_of_Polymerase_Chain_Reaction.pdf Rasheed, Abdul Khaliq and Kafafy, Raed and Faris, Waleed Fekry and Mohd. Salleh, Hamzah (2011) Enhancement of polymerase chain reaction using graphene nano-flakes. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
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institution |
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Online Access |
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English |
topic |
TJ Mechanical engineering and machinery TP248.13 Biotechnology |
spellingShingle |
TJ Mechanical engineering and machinery TP248.13 Biotechnology Rasheed, Abdul Khaliq Kafafy, Raed Faris, Waleed Fekry Mohd. Salleh, Hamzah Enhancement of polymerase chain reaction using graphene nano-flakes |
description |
The excellent heat transfer properties of nanoparticles
have potential applications in various fields including biology during the last two decades. Recently, the use of various nanoparticles in polymerase chain reaction (PCR) resulted in significant enhancement of its efficiency and specificity. In this research we have demonstrated the effects of a novel material, graphene nano-flakes on PCR. The rationale behind the use of graphene flakes is its unique physical and heat transfer properties. A number of experimental results including the effect of graphene flakes on denaturation of DNA and annealing step will also be discussed. The preliminary results clearly show that
enhanced heat transfer effect of nano-flakes augment PCR yield and ultimately overall enhancement in reaction efficiency. |
format |
Conference or Workshop Item |
author |
Rasheed, Abdul Khaliq Kafafy, Raed Faris, Waleed Fekry Mohd. Salleh, Hamzah |
author_facet |
Rasheed, Abdul Khaliq Kafafy, Raed Faris, Waleed Fekry Mohd. Salleh, Hamzah |
author_sort |
Rasheed, Abdul Khaliq |
title |
Enhancement of polymerase chain reaction using graphene nano-flakes |
title_short |
Enhancement of polymerase chain reaction using graphene nano-flakes |
title_full |
Enhancement of polymerase chain reaction using graphene nano-flakes |
title_fullStr |
Enhancement of polymerase chain reaction using graphene nano-flakes |
title_full_unstemmed |
Enhancement of polymerase chain reaction using graphene nano-flakes |
title_sort |
enhancement of polymerase chain reaction using graphene nano-flakes |
publishDate |
2011 |
url |
http://irep.iium.edu.my/16194/ http://irep.iium.edu.my/16194/ http://irep.iium.edu.my/16194/1/Enhancement_of_Polymerase_Chain_Reaction.pdf |
first_indexed |
2023-09-18T20:25:04Z |
last_indexed |
2023-09-18T20:25:04Z |
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1777408361823731712 |