On-column refolding of recombinant fungal endoglucanase I
Endoglucanase is an industrially important enzyme involved in degradation of cellulosic materials into constitutive monomers synergistically with other cellulase enzymes. In this study, overexpression of (His)6-tagged endoglucanase I from Fusarium oxysporum sp. in E. coli BL21(DE3) resulted to high...
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2011
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| Online Access: | http://irep.iium.edu.my/16211/ http://irep.iium.edu.my/16211/ http://irep.iium.edu.my/16211/1/On-column_Refolding_of_Recombinant_Fungal.pdf |
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iium-16211 |
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eprints |
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iium-162112012-02-02T07:16:34Z http://irep.iium.edu.my/16211/ On-column refolding of recombinant fungal endoglucanase I Aizat Jamaluddin, Mohd Jamil Mohd. Salleh, Hamzah TP248.13 Biotechnology Endoglucanase is an industrially important enzyme involved in degradation of cellulosic materials into constitutive monomers synergistically with other cellulase enzymes. In this study, overexpression of (His)6-tagged endoglucanase I from Fusarium oxysporum sp. in E. coli BL21(DE3) resulted to high tendency of endoglucanase I in the form of inactive inclusion bodies when the host cells were cultured between 25-37°C and induced by 0.05-0.20 mM (final concentration) IPTG. A simple and time-efficient on-column refolding scheme using IMAC aided by AKTA purifier, an automated fast protein liquid chromatography (FPLC) system afforded highly purified refolded recombinant endoglucanase I that was active in carboxymethyl cellulose (CMC) assay. The on-column refolding scheme is reliable and can be applied for efficient and robust method for lab-scale production of recombinant endoglucanase I. 2011 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/16211/1/On-column_Refolding_of_Recombinant_Fungal.pdf Aizat Jamaluddin, Mohd Jamil and Mohd. Salleh, Hamzah (2011) On-column refolding of recombinant fungal endoglucanase I. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Aizat Jamaluddin, Mohd Jamil Mohd. Salleh, Hamzah On-column refolding of recombinant fungal endoglucanase I |
| description |
Endoglucanase is an industrially important enzyme involved in degradation of cellulosic materials into constitutive monomers synergistically with other cellulase enzymes. In this study, overexpression of (His)6-tagged endoglucanase I from Fusarium oxysporum sp. in E. coli BL21(DE3) resulted to high tendency of endoglucanase I in the form of inactive inclusion bodies when the host cells were cultured between 25-37°C and induced by 0.05-0.20 mM (final concentration) IPTG. A simple and time-efficient on-column refolding scheme using IMAC aided by AKTA purifier, an automated fast protein liquid chromatography (FPLC) system afforded highly purified refolded recombinant endoglucanase I that was active in carboxymethyl cellulose (CMC) assay. The on-column refolding scheme is reliable and can be applied for efficient and robust method for lab-scale production of recombinant endoglucanase I. |
| format |
Conference or Workshop Item |
| author |
Aizat Jamaluddin, Mohd Jamil Mohd. Salleh, Hamzah |
| author_facet |
Aizat Jamaluddin, Mohd Jamil Mohd. Salleh, Hamzah |
| author_sort |
Aizat Jamaluddin, Mohd Jamil |
| title |
On-column refolding of recombinant fungal endoglucanase I |
| title_short |
On-column refolding of recombinant fungal endoglucanase I |
| title_full |
On-column refolding of recombinant fungal endoglucanase I |
| title_fullStr |
On-column refolding of recombinant fungal endoglucanase I |
| title_full_unstemmed |
On-column refolding of recombinant fungal endoglucanase I |
| title_sort |
on-column refolding of recombinant fungal endoglucanase i |
| publishDate |
2011 |
| url |
http://irep.iium.edu.my/16211/ http://irep.iium.edu.my/16211/ http://irep.iium.edu.my/16211/1/On-column_Refolding_of_Recombinant_Fungal.pdf |
| first_indexed |
2023-09-18T20:25:05Z |
| last_indexed |
2023-09-18T20:25:05Z |
| _version_ |
1777408363098800128 |