Degradation of phytate in rice bran by local bacteria phytase

Phytate (myo-inositol hexakisphosphates), a phosphorylated derivative of myo-inositol, is widely distributed in plants, particularly in cereals and legumes, such as corn, soybean, wheat bran, rice bran, cotton seeds, rape seeds and soybean with a concentration range between 0.4% to 6.4%. Rice bran,...

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Main Authors:	Mohd Yusoff, Noraini, Mohd. Salleh, Hamzah, Jamal, Parveen
Format:	Article
Language:	English
Published:	Research Journal of Chemistry and Environment 2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/16811/ http://irep.iium.edu.my/16811/ http://irep.iium.edu.my/16811/1/Degradation_of_phytate_in_rice_bran_Special_Issue_of_Research_Journal_of_Chemis.pdf

id	iium-16811
recordtype	eprints
spelling	iium-168112012-02-10T06:59:30Z http://irep.iium.edu.my/16811/ Degradation of phytate in rice bran by local bacteria phytase Mohd Yusoff, Noraini Mohd. Salleh, Hamzah Jamal, Parveen TP248.13 Biotechnology Phytate (myo-inositol hexakisphosphates), a phosphorylated derivative of myo-inositol, is widely distributed in plants, particularly in cereals and legumes, such as corn, soybean, wheat bran, rice bran, cotton seeds, rape seeds and soybean with a concentration range between 0.4% to 6.4%. Rice bran, a by-product of rice milling, is usually either discarded as a waste or used to feed poultry and livestock. In the present work, phytase was used to hydrolyze phytate to yield inorganic orthophosphate (Pi) and a series of lower phosphoric esters of myo-inositol and in some cases, free myo-inositol. Myo-inositol phosphate intermediates formed by the dephosphorylation of phytate (IP6) by wild type phytate-degrading enzymes were partially purified by a stepwise gradient on an anion exchanger. Two different phytate degrading enzymes, ASUIA 271 and ASUIA 30 were used to degrade the myo-inositol hexakisphosphate under optimized process conditions. Phosphate released from the myo-inositol phosphates and percentage yield of the myo-inositol phosphate produced were measured and recorded. ASUIA 30 gave higher yield of myo-inositol phosphates compared to ASUIA 271. Research Journal of Chemistry and Environment 2010-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/16811/1/Degradation_of_phytate_in_rice_bran_Special_Issue_of_Research_Journal_of_Chemis.pdf Mohd Yusoff, Noraini and Mohd. Salleh, Hamzah and Jamal, Parveen (2010) Degradation of phytate in rice bran by local bacteria phytase. Special Issue of Research Journal of Chemistry and Environment. pp. 361-365. ISSN 0972-0626 http://chemenviron.net
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohd Yusoff, Noraini Mohd. Salleh, Hamzah Jamal, Parveen Degradation of phytate in rice bran by local bacteria phytase
description	Phytate (myo-inositol hexakisphosphates), a phosphorylated derivative of myo-inositol, is widely distributed in plants, particularly in cereals and legumes, such as corn, soybean, wheat bran, rice bran, cotton seeds, rape seeds and soybean with a concentration range between 0.4% to 6.4%. Rice bran, a by-product of rice milling, is usually either discarded as a waste or used to feed poultry and livestock. In the present work, phytase was used to hydrolyze phytate to yield inorganic orthophosphate (Pi) and a series of lower phosphoric esters of myo-inositol and in some cases, free myo-inositol. Myo-inositol phosphate intermediates formed by the dephosphorylation of phytate (IP6) by wild type phytate-degrading enzymes were partially purified by a stepwise gradient on an anion exchanger. Two different phytate degrading enzymes, ASUIA 271 and ASUIA 30 were used to degrade the myo-inositol hexakisphosphate under optimized process conditions. Phosphate released from the myo-inositol phosphates and percentage yield of the myo-inositol phosphate produced were measured and recorded. ASUIA 30 gave higher yield of myo-inositol phosphates compared to ASUIA 271.
format	Article
author	Mohd Yusoff, Noraini Mohd. Salleh, Hamzah Jamal, Parveen
author_facet	Mohd Yusoff, Noraini Mohd. Salleh, Hamzah Jamal, Parveen
author_sort	Mohd Yusoff, Noraini
title	Degradation of phytate in rice bran by local bacteria phytase
title_short	Degradation of phytate in rice bran by local bacteria phytase
title_full	Degradation of phytate in rice bran by local bacteria phytase
title_fullStr	Degradation of phytate in rice bran by local bacteria phytase
title_full_unstemmed	Degradation of phytate in rice bran by local bacteria phytase
title_sort	degradation of phytate in rice bran by local bacteria phytase
publisher	Research Journal of Chemistry and Environment
publishDate	2010
url	http://irep.iium.edu.my/16811/ http://irep.iium.edu.my/16811/ http://irep.iium.edu.my/16811/1/Degradation_of_phytate_in_rice_bran_Special_Issue_of_Research_Journal_of_Chemis.pdf
first_indexed	2023-09-18T20:25:32Z
last_indexed	2023-09-18T20:25:32Z
_version_	1777408391032864768

Degradation of phytate in rice bran by local bacteria phytase

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