Isolation and analytical characterization of local Malaysian Leech Saliva extracts

Leech saliva contains biologically active compounds that are mainly proteins and peptides. In this study a modified and smooth extraction method of saliva was used without leeches’ scarification. UV and Bradford Assay protein methods showed that the saliva extract contains high concentrations of...

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Main Authors:	Alaama, Mohammed, Alnajjar, Manar, M. Abdualkader, Abdualrahman, Mohammad, Abbas, Merzouk, Ahmed
Format:	Article
Language:	English
Published:	IIUM Press 2011
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/17041/ http://irep.iium.edu.my/17041/ http://irep.iium.edu.my/17041/1/isolation.pdf

id	iium-17041
recordtype	eprints
spelling	iium-170412012-01-26T00:34:37Z http://irep.iium.edu.my/17041/ Isolation and analytical characterization of local Malaysian Leech Saliva extracts Alaama, Mohammed Alnajjar, Manar M. Abdualkader, Abdualrahman Mohammad, Abbas Merzouk, Ahmed R Medicine (General) Leech saliva contains biologically active compounds that are mainly proteins and peptides. In this study a modified and smooth extraction method of saliva was used without leeches’ scarification. UV and Bradford Assay protein methods showed that the saliva extract contains high concentrations of proteins. RP-HPLC chromatogram revealed that more than 30 different peaks were observed in leech saliva extract. Gel electrophoresis revealed the existence of proteins and peptides with different molecular weights. The gel showed up to 25 different bands. Comparison of gel electrophoresis data with protein database revealed the closeness of four molecular weights to known proteins from Hirudinaria leech family. Other proteins detected by gel electrophoresis may be related to completely new biologically active proteins and peptides in the saliva extract or to a modification (isoforms) of the existing ones or finally to a mixture of both IIUM Press 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/17041/1/isolation.pdf Alaama, Mohammed and Alnajjar, Manar and M. Abdualkader, Abdualrahman and Mohammad, Abbas and Merzouk, Ahmed (2011) Isolation and analytical characterization of local Malaysian Leech Saliva extracts. IIUM Engineering Journal, 12 (4). pp. 51-59. ISSN 1511-788X http://www.iium.edu.my/ejournal/index.php/iiumej/article/view/182/201
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Alaama, Mohammed Alnajjar, Manar M. Abdualkader, Abdualrahman Mohammad, Abbas Merzouk, Ahmed Isolation and analytical characterization of local Malaysian Leech Saliva extracts
description	Leech saliva contains biologically active compounds that are mainly proteins and peptides. In this study a modified and smooth extraction method of saliva was used without leeches’ scarification. UV and Bradford Assay protein methods showed that the saliva extract contains high concentrations of proteins. RP-HPLC chromatogram revealed that more than 30 different peaks were observed in leech saliva extract. Gel electrophoresis revealed the existence of proteins and peptides with different molecular weights. The gel showed up to 25 different bands. Comparison of gel electrophoresis data with protein database revealed the closeness of four molecular weights to known proteins from Hirudinaria leech family. Other proteins detected by gel electrophoresis may be related to completely new biologically active proteins and peptides in the saliva extract or to a modification (isoforms) of the existing ones or finally to a mixture of both
format	Article
author	Alaama, Mohammed Alnajjar, Manar M. Abdualkader, Abdualrahman Mohammad, Abbas Merzouk, Ahmed
author_facet	Alaama, Mohammed Alnajjar, Manar M. Abdualkader, Abdualrahman Mohammad, Abbas Merzouk, Ahmed
author_sort	Alaama, Mohammed
title	Isolation and analytical characterization of local Malaysian Leech Saliva extracts
title_short	Isolation and analytical characterization of local Malaysian Leech Saliva extracts
title_full	Isolation and analytical characterization of local Malaysian Leech Saliva extracts
title_fullStr	Isolation and analytical characterization of local Malaysian Leech Saliva extracts
title_full_unstemmed	Isolation and analytical characterization of local Malaysian Leech Saliva extracts
title_sort	isolation and analytical characterization of local malaysian leech saliva extracts
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/17041/ http://irep.iium.edu.my/17041/ http://irep.iium.edu.my/17041/1/isolation.pdf
first_indexed	2023-09-18T20:25:51Z
last_indexed	2023-09-18T20:25:51Z
_version_	1777408411052277760

Isolation and analytical characterization of local Malaysian Leech Saliva extracts

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