Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B

p53 gene has been hailed to have important roles in maintaining genomic integrity. The critical roles of this tumor suppressor in cell cycle, DNA repair, and opoptosis profoundly contribute to development of cancer. p53 mutations have been found in almost fifty percent of all cancer types, including...

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Main Authors:	Hayati, Rahma Fitri, Deny Susanti, Darnis, Arief Ichwan, Solachuddin Jauhari
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/18158/ http://irep.iium.edu.my/18158/ http://irep.iium.edu.my/18158/1/Growth_suppression.pdf

id	iium-18158
recordtype	eprints
spelling	iium-181582012-02-09T13:53:53Z http://irep.iium.edu.my/18158/ Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B Hayati, Rahma Fitri Deny Susanti, Darnis Arief Ichwan, Solachuddin Jauhari Q Science (General) p53 gene has been hailed to have important roles in maintaining genomic integrity. The critical roles of this tumor suppressor in cell cycle, DNA repair, and opoptosis profoundly contribute to development of cancer. p53 mutations have been found in almost fifty percent of all cancer types, including lung cancers. Hence, the idea of restoring the normal function of p53 gene by exogeous p53 replacement therapy has been discussed and investigated in order to overcome cancer. However, introduction of wild-type p53 protein is unable ot induce apoptosis in all tumor cases, at least in part, due to their resistance to exogenous p53. p73Bhas been regarded as one of the strongest candidate as it is similar to p53 in many aspects: structural homology, transactivation of p53-downstrean genes, and induction of apoptosis. It has been reported that a similar therapeutic strategy can be successfully applied in p73 activation as well. Interestingly, the mutation of this gene has been rarely found in cancer, suggesting different mechanism or regulation pathway of this gene. The aim of this study is to compare the tumor suppressor activity of p53 and p73B, on p53 and p73B constructed in pCMV plasmid was conducted through chemical mediated transfection. Introduction of exogenous expression of p53 significantly suppress colony formation of H1299 cells under G418 selection while p73B could partially suppress the colony formation. Consistently, exogenous p53 and p73B raise expression of p21/Waf gene. These results show overlapping regulation performed by p73B which suggest its application as alternative candidate in gene therapy. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/18158/1/Growth_suppression.pdf Hayati, Rahma Fitri and Deny Susanti, Darnis and Arief Ichwan, Solachuddin Jauhari (2010) Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B. In: 3rd International Conference on Advancement in Science and Technology (iCAST) 2010, 27-29 November 2010, Vistana Hotel, Kuantan, Pahang. http://www.iium.edu.my/icast/2010/index.php?option=com_content&view=article&id=14&Itemid=17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Hayati, Rahma Fitri Deny Susanti, Darnis Arief Ichwan, Solachuddin Jauhari Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B
description	p53 gene has been hailed to have important roles in maintaining genomic integrity. The critical roles of this tumor suppressor in cell cycle, DNA repair, and opoptosis profoundly contribute to development of cancer. p53 mutations have been found in almost fifty percent of all cancer types, including lung cancers. Hence, the idea of restoring the normal function of p53 gene by exogeous p53 replacement therapy has been discussed and investigated in order to overcome cancer. However, introduction of wild-type p53 protein is unable ot induce apoptosis in all tumor cases, at least in part, due to their resistance to exogenous p53. p73Bhas been regarded as one of the strongest candidate as it is similar to p53 in many aspects: structural homology, transactivation of p53-downstrean genes, and induction of apoptosis. It has been reported that a similar therapeutic strategy can be successfully applied in p73 activation as well. Interestingly, the mutation of this gene has been rarely found in cancer, suggesting different mechanism or regulation pathway of this gene. The aim of this study is to compare the tumor suppressor activity of p53 and p73B, on p53 and p73B constructed in pCMV plasmid was conducted through chemical mediated transfection. Introduction of exogenous expression of p53 significantly suppress colony formation of H1299 cells under G418 selection while p73B could partially suppress the colony formation. Consistently, exogenous p53 and p73B raise expression of p21/Waf gene. These results show overlapping regulation performed by p73B which suggest its application as alternative candidate in gene therapy.
format	Conference or Workshop Item
author	Hayati, Rahma Fitri Deny Susanti, Darnis Arief Ichwan, Solachuddin Jauhari
author_facet	Hayati, Rahma Fitri Deny Susanti, Darnis Arief Ichwan, Solachuddin Jauhari
author_sort	Hayati, Rahma Fitri
title	Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B
title_short	Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B
title_full	Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B
title_fullStr	Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B
title_full_unstemmed	Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B
title_sort	growth suppression of non-small lung carcinoma cell h1299 transfected with p53 and p73b
publishDate	2010
url	http://irep.iium.edu.my/18158/ http://irep.iium.edu.my/18158/ http://irep.iium.edu.my/18158/1/Growth_suppression.pdf
first_indexed	2023-09-18T20:27:11Z
last_indexed	2023-09-18T20:27:11Z
_version_	1777408495122907136

Growth suppression of non-small lung carcinoma cell H1299 transfected with p53 and p73B

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