Metal chelating activity of Centella asiatica

Centella asiatica (CA), a potent antioxidant, was studied for its potential as a neuroprotective agent to fight against oxidative damage by reactive oxygen species (ROS) to neuronal cells which eventually lead to neurodegenerative diseases such as Alzheimer's and Parkinson disease. The build-up...

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Main Authors:	Mohd Salim, Roshan Jahn, Amid, Azura, Adenan, Mohd Ilham, Sued, Anee Suryani
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	R Medicine (General) RB Pathology
Online Access:	http://irep.iium.edu.my/19491/ http://irep.iium.edu.my/19491/ http://irep.iium.edu.my/19491/1/Metal_chelating.pdf

id	iium-19491
recordtype	eprints
spelling	iium-194912013-06-18T04:07:35Z http://irep.iium.edu.my/19491/ Metal chelating activity of Centella asiatica Mohd Salim, Roshan Jahn Amid, Azura Adenan, Mohd Ilham Sued, Anee Suryani R Medicine (General) RB Pathology Centella asiatica (CA), a potent antioxidant, was studied for its potential as a neuroprotective agent to fight against oxidative damage by reactive oxygen species (ROS) to neuronal cells which eventually lead to neurodegenerative diseases such as Alzheimer's and Parkinson disease. The build-up of an iron gradient in conjunction with ROS is thought to constitute a major trigger in neuronal toxicity and demise in these diseases. The limited number of neuroprotective study carried out so far indicate the iron chelation therapy could be a viable neuroprotective approach for neurodegenerative disorders. The metal chelating potency can be determined by assessing the ability of neuroprotective agents to compete with ferrozine for Fe(II) ions. Ferrozine forms complexes with Fe(II) producing a red color that can be measured quantitatively at 562 nm and addition of iron chelators interferes with the formation of ferrous and ferrozine complexes in a concentration-dependent manner. The IC50 value, concentration re quired for 50% inhibition of the iron-ferrozine complex, was measured. Methanolic and aqueous extract of CA was prepared by conventional soxhlet extraction (40-60C, ratio: 1:30(w/v), 15 hours). The IC50 of methanolic and aqueous extract obtained was 0.26mg/ml and 0.69mg/ml respectively. The (-)-epigallocatechin-3-gallate (AGCG), Major component of green tea reported to have induce neuroprotection and neurorescue both in vitro and in vivo was used as positive control. Further, Response Surface Methodology was used to determine the optimum CA extraction condition that give optimum metal chelating activity. Experiments were designed according to Central Composite Design with four factors (time, temperature, ratio of raw material to solvent and agitation speed) and six central points that derived total 30 runs. Run 17 (25C, ratio: 1:45(w/v), 1.5 hours at 200 rpm) gave the optimum chelating activity with IC50 0.093 mg/ml. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/19491/1/Metal_chelating.pdf Mohd Salim, Roshan Jahn and Amid, Azura and Adenan, Mohd Ilham and Sued, Anee Suryani (2010) Metal chelating activity of Centella asiatica. In: 3rd International Conference on Advancement in Science and Technology (iCAST) 2010, 27-29 November 2010, Vistana Hotel, Kuantan, Pahang. http://www.iium.edu.my/icast/2010/index.php?option=com_content&view=article&id=14&Itemid=17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General) RB Pathology
spellingShingle	R Medicine (General) RB Pathology Mohd Salim, Roshan Jahn Amid, Azura Adenan, Mohd Ilham Sued, Anee Suryani Metal chelating activity of Centella asiatica
description	Centella asiatica (CA), a potent antioxidant, was studied for its potential as a neuroprotective agent to fight against oxidative damage by reactive oxygen species (ROS) to neuronal cells which eventually lead to neurodegenerative diseases such as Alzheimer's and Parkinson disease. The build-up of an iron gradient in conjunction with ROS is thought to constitute a major trigger in neuronal toxicity and demise in these diseases. The limited number of neuroprotective study carried out so far indicate the iron chelation therapy could be a viable neuroprotective approach for neurodegenerative disorders. The metal chelating potency can be determined by assessing the ability of neuroprotective agents to compete with ferrozine for Fe(II) ions. Ferrozine forms complexes with Fe(II) producing a red color that can be measured quantitatively at 562 nm and addition of iron chelators interferes with the formation of ferrous and ferrozine complexes in a concentration-dependent manner. The IC50 value, concentration re quired for 50% inhibition of the iron-ferrozine complex, was measured. Methanolic and aqueous extract of CA was prepared by conventional soxhlet extraction (40-60C, ratio: 1:30(w/v), 15 hours). The IC50 of methanolic and aqueous extract obtained was 0.26mg/ml and 0.69mg/ml respectively. The (-)-epigallocatechin-3-gallate (AGCG), Major component of green tea reported to have induce neuroprotection and neurorescue both in vitro and in vivo was used as positive control. Further, Response Surface Methodology was used to determine the optimum CA extraction condition that give optimum metal chelating activity. Experiments were designed according to Central Composite Design with four factors (time, temperature, ratio of raw material to solvent and agitation speed) and six central points that derived total 30 runs. Run 17 (25C, ratio: 1:45(w/v), 1.5 hours at 200 rpm) gave the optimum chelating activity with IC50 0.093 mg/ml.
format	Conference or Workshop Item
author	Mohd Salim, Roshan Jahn Amid, Azura Adenan, Mohd Ilham Sued, Anee Suryani
author_facet	Mohd Salim, Roshan Jahn Amid, Azura Adenan, Mohd Ilham Sued, Anee Suryani
author_sort	Mohd Salim, Roshan Jahn
title	Metal chelating activity of Centella asiatica
title_short	Metal chelating activity of Centella asiatica
title_full	Metal chelating activity of Centella asiatica
title_fullStr	Metal chelating activity of Centella asiatica
title_full_unstemmed	Metal chelating activity of Centella asiatica
title_sort	metal chelating activity of centella asiatica
publishDate	2010
url	http://irep.iium.edu.my/19491/ http://irep.iium.edu.my/19491/ http://irep.iium.edu.my/19491/1/Metal_chelating.pdf
first_indexed	2023-09-18T20:29:06Z
last_indexed	2023-09-18T20:29:06Z
_version_	1777408615718584320

Metal chelating activity of Centella asiatica

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