Antioxidant activity of leaves of Calophyllum Rubiginosum

Calophyllum species have been used in traditional medicine for their therapeutic values for many years. This use was explained by several previous studies where antimicrobial, antifungal, anti-HIV and anti-cancer and antioxidant compounds were isolated from several Calophyllum species. Approach: The...

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Main Authors:	Bakhtiar, M. Taher, Darnis, Deny Susanti, Arief Ichwan, Solachuddin Jauhari, Ahmad, Farediah
Format:	Article
Language:	English
Published:	Science Publications 2010
Subjects:	RM Therapeutics. Pharmacology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/1981/ http://irep.iium.edu.my/1981/ http://irep.iium.edu.my/1981/ http://irep.iium.edu.my/1981/1/ajassp.2010.1305.1309.pdf

id	iium-1981
recordtype	eprints
spelling	iium-19812012-05-06T06:42:43Z http://irep.iium.edu.my/1981/ Antioxidant activity of leaves of Calophyllum Rubiginosum Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Ahmad, Farediah RM Therapeutics. Pharmacology RS Pharmacy and materia medica Calophyllum species have been used in traditional medicine for their therapeutic values for many years. This use was explained by several previous studies where antimicrobial, antifungal, anti-HIV and anti-cancer and antioxidant compounds were isolated from several Calophyllum species. Approach: The leaves were extracted with n-hexane (hexane), dichloromethane (DCM) and methanol using soxhlet apparatus. Total phenolic contents of each extract were evaluated using Folin Ciocalteau Reagent (FRC) while Ferric Thiocyanate (FTC) and 2,2-Diphenyl-1 Picrylhydrazyl (DPPH) were used for lipid peroxidation and free radical scavenging activity respectively. Results: As the results, methanol extracts (5 mg mL-1) has higher phenolic contents of 66.2 μg mg-1 Gallic Acid Equivalent (GAE) compared to n-hexane and DCM extracts with phenolic content of 50.5 and 52.5 μg mg-1 GAE respectively. In FTC method, methanol had the lower absorbance values which indicate high antioxidant activities. In DPPH radical scavenging test however, DCM extract showed high antioxidant activity with an IC50 value of 0.11mg mL-1, followed by methanol and hexane with IC50 values of 0.23 and 4.5 mg mL-1 respectively. The IC50 of standards were 0.74 and 0.56 mg mL-1 for ascorbic acid and α tocopherol, respectively. Conclusion: Finally, the positive outcome of the research can be further tested in the future to ascertain the active compound of the leaves of C. rubiginosum. Science Publications 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/1981/1/ajassp.2010.1305.1309.pdf Bakhtiar, M. Taher and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari and Ahmad, Farediah (2010) Antioxidant activity of leaves of Calophyllum Rubiginosum. American Journal of Applied Sciences , 7 (10). pp. 1305-1309. ISSN 1554-3641(O), 1546-9239 (P) http://thescipub.com/ajas.toc DOI: 10.3844/ajassp.2010.1305.1309
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology RS Pharmacy and materia medica
spellingShingle	RM Therapeutics. Pharmacology RS Pharmacy and materia medica Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Ahmad, Farediah Antioxidant activity of leaves of Calophyllum Rubiginosum
description	Calophyllum species have been used in traditional medicine for their therapeutic values for many years. This use was explained by several previous studies where antimicrobial, antifungal, anti-HIV and anti-cancer and antioxidant compounds were isolated from several Calophyllum species. Approach: The leaves were extracted with n-hexane (hexane), dichloromethane (DCM) and methanol using soxhlet apparatus. Total phenolic contents of each extract were evaluated using Folin Ciocalteau Reagent (FRC) while Ferric Thiocyanate (FTC) and 2,2-Diphenyl-1 Picrylhydrazyl (DPPH) were used for lipid peroxidation and free radical scavenging activity respectively. Results: As the results, methanol extracts (5 mg mL-1) has higher phenolic contents of 66.2 μg mg-1 Gallic Acid Equivalent (GAE) compared to n-hexane and DCM extracts with phenolic content of 50.5 and 52.5 μg mg-1 GAE respectively. In FTC method, methanol had the lower absorbance values which indicate high antioxidant activities. In DPPH radical scavenging test however, DCM extract showed high antioxidant activity with an IC50 value of 0.11mg mL-1, followed by methanol and hexane with IC50 values of 0.23 and 4.5 mg mL-1 respectively. The IC50 of standards were 0.74 and 0.56 mg mL-1 for ascorbic acid and α tocopherol, respectively. Conclusion: Finally, the positive outcome of the research can be further tested in the future to ascertain the active compound of the leaves of C. rubiginosum.
format	Article
author	Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Ahmad, Farediah
author_facet	Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Ahmad, Farediah
author_sort	Bakhtiar, M. Taher
title	Antioxidant activity of leaves of Calophyllum Rubiginosum
title_short	Antioxidant activity of leaves of Calophyllum Rubiginosum
title_full	Antioxidant activity of leaves of Calophyllum Rubiginosum
title_fullStr	Antioxidant activity of leaves of Calophyllum Rubiginosum
title_full_unstemmed	Antioxidant activity of leaves of Calophyllum Rubiginosum
title_sort	antioxidant activity of leaves of calophyllum rubiginosum
publisher	Science Publications
publishDate	2010
url	http://irep.iium.edu.my/1981/ http://irep.iium.edu.my/1981/ http://irep.iium.edu.my/1981/ http://irep.iium.edu.my/1981/1/ajassp.2010.1305.1309.pdf
first_indexed	2023-09-18T20:09:31Z
last_indexed	2023-09-18T20:09:31Z
_version_	1777407383710990336

Antioxidant activity of leaves of Calophyllum Rubiginosum

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