Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast

Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast

This study was carried out to observe the biocompatibility of gentamicin-coated hydroxyapatite (HA) on osteoblast using fluorescence microscopy. The specific objective was to observe the viability of the osteoblast on the gentamicin-coated hydroxyapatite (HA) and to determine the effect of the bioma...

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Main Authors: Che Seman, Che Nor Zarida, Othman, Fauziah, Moin, Saidi, Wong, C. C., Besar, Idris, Mustaffa, Rusnah, Goriman Khan, Mohd Azam Khan, Ahmad, Azfar Rizal, Zulkifly, Ahmad Hafiz
Format: Article
Language:English
Published: Universiti Putra Malaysia 2010
Subjects:
RD701 Orthopedics
Online Access:http://irep.iium.edu.my/19993/
http://irep.iium.edu.my/19993/1/Fluorescence_microscopy_on_the_biocompatibility_of_gentamicin-coated_hydroxyapatite_%28HA%29_material_on_osteoblast.pdf
id iium-19993
recordtype eprints
spelling iium-199932015-04-17T08:32:42Z http://irep.iium.edu.my/19993/ Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast Che Seman, Che Nor Zarida Othman, Fauziah Moin, Saidi Wong, C. C. Besar, Idris Mustaffa, Rusnah Goriman Khan, Mohd Azam Khan Ahmad, Azfar Rizal Zulkifly, Ahmad Hafiz RD701 Orthopedics This study was carried out to observe the biocompatibility of gentamicin-coated hydroxyapatite (HA) on osteoblast using fluorescence microscopy. The specific objective was to observe the viability of the osteoblast on the gentamicin-coated hydroxyapatite (HA) and to determine the effect of the biomaterial coated with gentamicin on the osteoblast. Osteoblast cell lines were cultured and maintained in complete medium, 1:1 HAM's F12 Medium Dulbecco's modified Eagle's medium without phenol red (DMEM) and incubated at 37°C in a 5% CO2. The cell lines were treated with different concentration of gentamicin-coated hydroxyapatite and the interactions of the antibiotic beads against osteoblast were tested using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The MTT assay results indicated that varying concentrations of gentamicin coated HA from 0.1 mg/ml to 10 mg/ml did not significantly affect viability of osteoblast. By employing fluorescence microscopy, the morphology of osteoblast observed appeared red in color which indicated that the osteoblast was viable on biomaterial. The pore size of hydroxyapatite is between 150 to 350 nm. This preliminary result suggested that the gentamicin-coated HA had a good biocompatibility towards osteoblast. Universiti Putra Malaysia 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/19993/1/Fluorescence_microscopy_on_the_biocompatibility_of_gentamicin-coated_hydroxyapatite_%28HA%29_material_on_osteoblast.pdf Che Seman, Che Nor Zarida and Othman, Fauziah and Moin, Saidi and Wong, C. C. and Besar, Idris and Mustaffa, Rusnah and Goriman Khan, Mohd Azam Khan and Ahmad, Azfar Rizal and Zulkifly, Ahmad Hafiz (2010) Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast. Malaysian Journal of Microscopy, 6 (1). pp. 1-8. ISSN 1823-7010
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RD701 Orthopedics
spellingShingle RD701 Orthopedics
Che Seman, Che Nor Zarida
Othman, Fauziah
Moin, Saidi
Wong, C. C.
Besar, Idris
Mustaffa, Rusnah
Goriman Khan, Mohd Azam Khan
Ahmad, Azfar Rizal
Zulkifly, Ahmad Hafiz
Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast
description This study was carried out to observe the biocompatibility of gentamicin-coated hydroxyapatite (HA) on osteoblast using fluorescence microscopy. The specific objective was to observe the viability of the osteoblast on the gentamicin-coated hydroxyapatite (HA) and to determine the effect of the biomaterial coated with gentamicin on the osteoblast. Osteoblast cell lines were cultured and maintained in complete medium, 1:1 HAM's F12 Medium Dulbecco's modified Eagle's medium without phenol red (DMEM) and incubated at 37°C in a 5% CO2. The cell lines were treated with different concentration of gentamicin-coated hydroxyapatite and the interactions of the antibiotic beads against osteoblast were tested using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The MTT assay results indicated that varying concentrations of gentamicin coated HA from 0.1 mg/ml to 10 mg/ml did not significantly affect viability of osteoblast. By employing fluorescence microscopy, the morphology of osteoblast observed appeared red in color which indicated that the osteoblast was viable on biomaterial. The pore size of hydroxyapatite is between 150 to 350 nm. This preliminary result suggested that the gentamicin-coated HA had a good biocompatibility towards osteoblast.
format Article
author Che Seman, Che Nor Zarida
Othman, Fauziah
Moin, Saidi
Wong, C. C.
Besar, Idris
Mustaffa, Rusnah
Goriman Khan, Mohd Azam Khan
Ahmad, Azfar Rizal
Zulkifly, Ahmad Hafiz
author_facet Che Seman, Che Nor Zarida
Othman, Fauziah
Moin, Saidi
Wong, C. C.
Besar, Idris
Mustaffa, Rusnah
Goriman Khan, Mohd Azam Khan
Ahmad, Azfar Rizal
Zulkifly, Ahmad Hafiz
author_sort Che Seman, Che Nor Zarida
title Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast
title_short Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast
title_full Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast
title_fullStr Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast
title_full_unstemmed Fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (HA) material on osteoblast
title_sort fluorescence microscopy on the biocompatibility of gentamicin-coated hydroxyapatite (ha) material on osteoblast
publisher Universiti Putra Malaysia
publishDate 2010
url http://irep.iium.edu.my/19993/
http://irep.iium.edu.my/19993/1/Fluorescence_microscopy_on_the_biocompatibility_of_gentamicin-coated_hydroxyapatite_%28HA%29_material_on_osteoblast.pdf
first_indexed 2023-09-18T20:29:58Z
last_indexed 2023-09-18T20:29:58Z
_version_ 1777408670228807680

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