Effects of cell immobilization to the phytate-degrading enzyme activity

The use of immobilized whole microhial cells and/or organelles eliminates lhc often tedious. lime consuming. and expensi\'e sleps invohed in isolation and purificatioll of imraccllular enzymes. II al>o lends 10 enhance the stability of lhe enzyme by relaining its nall.....l catalytic surroun...

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Main Authors:	Mohamad Puad, Noor Illi, Farouk, Abd Alaziem, Salleh, Hamzah Mohd.
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/20776/ http://irep.iium.edu.my/20776/ http://irep.iium.edu.my/20776/1/EFFECTS_OF_CELL_IMMOBILIZATION_TO_THE_PHYTATE-_DEGRADING_ENZYME_ACTNITY.pdf

id	iium-20776
recordtype	eprints
spelling	iium-207762012-11-27T06:53:38Z http://irep.iium.edu.my/20776/ Effects of cell immobilization to the phytate-degrading enzyme activity Mohamad Puad, Noor Illi Farouk, Abd Alaziem Salleh, Hamzah Mohd. TP248.13 Biotechnology The use of immobilized whole microhial cells and/or organelles eliminates lhc often tedious. lime consuming. and expensi\'e sleps invohed in isolation and purificatioll of imraccllular enzymes. II al>o lends 10 enhance the stability of lhe enzyme by relaining its nall.....l catalytic surroundings during immobilizalion and subsequent cominuous operalion. A baclerium isolated from Malaysian waslewatn pnxlucing a 1l0,-e1 phyrate-degrading enzyme namely PhyFAUIAI was immobilized in alginate gel beads. PhyFAUIA 1 is a periplasmatic enzyme and highly substralespecific 10 phytale. The enzyme aClivity wa~ invesligaled upon cell immobilization. No shift in pH oplima of phytase of immobilized cells was obsen·ed. 110wever. the optimum lcmperaturcbwas shifted 10 50°C, which was 15°C lower lhan free PhyFAUIAI enzyme. Immobilized cells were found 10 be more stable in wider range of pll and tcmpe-ramr.: compared to free cells. The results presenled in this work show lhe potential for using immobilized cells to produce ditrerent "~nJ-inosilOl phosphates intennediales IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20776/1/EFFECTS_OF_CELL_IMMOBILIZATION_TO_THE_PHYTATE-_DEGRADING_ENZYME_ACTNITY.pdf Mohamad Puad, Noor Illi and Farouk, Abd Alaziem and Salleh, Hamzah Mohd. (2011) Effects of cell immobilization to the phytate-degrading enzyme activity. In: Current Research and Development in Biotechnology Engineering at IIUM (volume II). IIUM Press, Kuala Lumpur, pp. 137-143. ISBN 978-967-418-151-2 http://rms.research.iium.edu.my/bookstore/default.aspx
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mohamad Puad, Noor Illi Farouk, Abd Alaziem Salleh, Hamzah Mohd. Effects of cell immobilization to the phytate-degrading enzyme activity
description	The use of immobilized whole microhial cells and/or organelles eliminates lhc often tedious. lime consuming. and expensi\'e sleps invohed in isolation and purificatioll of imraccllular enzymes. II al>o lends 10 enhance the stability of lhe enzyme by relaining its nall.....l catalytic surroundings during immobilizalion and subsequent cominuous operalion. A baclerium isolated from Malaysian waslewatn pnxlucing a 1l0,-e1 phyrate-degrading enzyme namely PhyFAUIAI was immobilized in alginate gel beads. PhyFAUIA 1 is a periplasmatic enzyme and highly substralespecific 10 phytale. The enzyme aClivity wa~ invesligaled upon cell immobilization. No shift in pH oplima of phytase of immobilized cells was obsen·ed. 110wever. the optimum lcmperaturcbwas shifted 10 50°C, which was 15°C lower lhan free PhyFAUIAI enzyme. Immobilized cells were found 10 be more stable in wider range of pll and tcmpe-ramr.: compared to free cells. The results presenled in this work show lhe potential for using immobilized cells to produce ditrerent "~nJ-inosilOl phosphates intennediales
format	Book Chapter
author	Mohamad Puad, Noor Illi Farouk, Abd Alaziem Salleh, Hamzah Mohd.
author_facet	Mohamad Puad, Noor Illi Farouk, Abd Alaziem Salleh, Hamzah Mohd.
author_sort	Mohamad Puad, Noor Illi
title	Effects of cell immobilization to the phytate-degrading enzyme activity
title_short	Effects of cell immobilization to the phytate-degrading enzyme activity
title_full	Effects of cell immobilization to the phytate-degrading enzyme activity
title_fullStr	Effects of cell immobilization to the phytate-degrading enzyme activity
title_full_unstemmed	Effects of cell immobilization to the phytate-degrading enzyme activity
title_sort	effects of cell immobilization to the phytate-degrading enzyme activity
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/20776/ http://irep.iium.edu.my/20776/ http://irep.iium.edu.my/20776/1/EFFECTS_OF_CELL_IMMOBILIZATION_TO_THE_PHYTATE-_DEGRADING_ENZYME_ACTNITY.pdf
first_indexed	2023-09-18T20:31:23Z
last_indexed	2023-09-18T20:31:23Z
_version_	1777408758954065920

Effects of cell immobilization to the phytate-degrading enzyme activity

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