Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum

Xanthine oxidase (XO) is an enzyme that catalyses the oxidation of hypoxanthine and xanthine to uric acid, Which plays a crucial role in gout. Gout is distinguished by recurring attacks of joint inflammation. It is influenced by a high dietary intake of foods rich in nucleic acid. Our preliminary...

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Main Authors:	Jamal, Parveen, Amid, Azura, Bahardin, Rasidi, Azmi, Saiful Mohammad Nizam
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/20977/ http://irep.iium.edu.my/20977/ http://irep.iium.edu.my/20977/1/optimization_of_process_-_C29.pdf

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recordtype	eprints
spelling	iium-209772012-07-05T00:21:39Z http://irep.iium.edu.my/20977/ Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum Jamal, Parveen Amid, Azura Bahardin, Rasidi Azmi, Saiful Mohammad Nizam TP248.13 Biotechnology Xanthine oxidase (XO) is an enzyme that catalyses the oxidation of hypoxanthine and xanthine to uric acid, Which plays a crucial role in gout. Gout is distinguished by recurring attacks of joint inflammation. It is influenced by a high dietary intake of foods rich in nucleic acid. Our preliminary screening studies have shown that methanolic extract of Lycopersicon esculentum leaves demonstrated the highest inhibition of XO. Thus, it was subjected to optimization of extraction parameters, which include extraction temperature, extraction time, agitation speed and ratio of the solvent to plant matenals. By using Central Composite Design (CCD) for the optimization, various XO inhibitory activities can be monitored. The optimum conditions as stated by further numerical analysis of the responses using Design Expert v.6.0.8 revealed that the maximum XO inhibitory activity is 88.41% at extraction temperature of 30°C, extraction time of 25.15 hour, extraction speed of 25 rpm, and solvent to plant ratio of 10.71 ml/l g. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20977/1/optimization_of_process_-_C29.pdf Jamal, Parveen and Amid, Azura and Bahardin, Rasidi and Azmi, Saiful Mohammad Nizam (2011) Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 226-236. ISBN 9789674181444 http://rms.research.iium.edu.my/bookstore/default.aspx
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Jamal, Parveen Amid, Azura Bahardin, Rasidi Azmi, Saiful Mohammad Nizam Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum
description	Xanthine oxidase (XO) is an enzyme that catalyses the oxidation of hypoxanthine and xanthine to uric acid, Which plays a crucial role in gout. Gout is distinguished by recurring attacks of joint inflammation. It is influenced by a high dietary intake of foods rich in nucleic acid. Our preliminary screening studies have shown that methanolic extract of Lycopersicon esculentum leaves demonstrated the highest inhibition of XO. Thus, it was subjected to optimization of extraction parameters, which include extraction temperature, extraction time, agitation speed and ratio of the solvent to plant matenals. By using Central Composite Design (CCD) for the optimization, various XO inhibitory activities can be monitored. The optimum conditions as stated by further numerical analysis of the responses using Design Expert v.6.0.8 revealed that the maximum XO inhibitory activity is 88.41% at extraction temperature of 30°C, extraction time of 25.15 hour, extraction speed of 25 rpm, and solvent to plant ratio of 10.71 ml/l g.
format	Book Chapter
author	Jamal, Parveen Amid, Azura Bahardin, Rasidi Azmi, Saiful Mohammad Nizam
author_facet	Jamal, Parveen Amid, Azura Bahardin, Rasidi Azmi, Saiful Mohammad Nizam
author_sort	Jamal, Parveen
title	Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum
title_short	Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum
title_full	Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum
title_fullStr	Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum
title_full_unstemmed	Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum
title_sort	optimization of process parameters for extraction of xanthine oxidase inhibitor (xoi) from lycopersicon esculentum
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/20977/ http://irep.iium.edu.my/20977/ http://irep.iium.edu.my/20977/1/optimization_of_process_-_C29.pdf
first_indexed	2023-09-18T20:31:43Z
last_indexed	2023-09-18T20:31:43Z
_version_	1777408780152078336

Optimization of process parameters for extraction of xanthine oxidase inhibitor (XOI) from lycopersicon esculentum

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