Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas)

Horseshoe crabs provide the important biomedical products, Limulus Amebocyte Lysate (LAL) and Tachypleus Amebocyte Lysate (TAL), which are produced by lysing the blood cells (amebocytes) using endotoxin-free distilled water. LAL/TAL is used to detect and quantify lipopolysaccharide (endotoxin), an i...

Full description

Bibliographic Details
Main Authors:	John, Akbar, Khan Chowdhury, Ahmed Jalal, Kasim, Zaleha, Amstrong, Peter B., Yunus, Kamaruzzaman
Format:	Article
Language:	English
Published:	Taylor & Francis 2011
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/22563/ http://irep.iium.edu.my/22563/ http://irep.iium.edu.my/22563/ http://irep.iium.edu.my/22563/1/10236244.2011.pdf

id	iium-22563
recordtype	eprints
spelling	iium-225632017-12-07T05:29:10Z http://irep.iium.edu.my/22563/ Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas) John, Akbar Khan Chowdhury, Ahmed Jalal Kasim, Zaleha Amstrong, Peter B. Yunus, Kamaruzzaman Q Science (General) Horseshoe crabs provide the important biomedical products, Limulus Amebocyte Lysate (LAL) and Tachypleus Amebocyte Lysate (TAL), which are produced by lysing the blood cells (amebocytes) using endotoxin-free distilled water. LAL/TAL is used to detect and quantify lipopolysaccharide (endotoxin), an important toxin of Gram-negative bacteria. Horseshoe crabs are the sole source for LAL/TAL and their fishery for the production of LAL/TAL is of concern to the sustained management of the species. This study investigated the mortality of the Malaysian horseshoe crab (Tachypleus gigas) related to blood extraction, with the aim of providing a basis for the sustainable management of the local fishery of this animal. We showed that the mortality rate of T. gigas due to the bleeding process is slightly higher than the values which has previously been reported for its conspecific, the American horseshoe crab (Limulus polyphemus). Overall mortalities of bled horseshoe crabs were 17.8% (male = 18.75%; female = 17.2%), while mortalities of unbled crabs were 3.5%. The quantification of the mortality due to the extent of bleeding commensurate with the commercial production of LAL/TAL provides a basis for the rational management of T. gigas in situations where harvest for LAL/TAL production is imposed on local populations of the animal. Taylor & Francis 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/22563/1/10236244.2011.pdf John, Akbar and Khan Chowdhury, Ahmed Jalal and Kasim, Zaleha and Amstrong, Peter B. and Yunus, Kamaruzzaman (2011) Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas). Marine and Freshwater Behaviour and Physiology, 44 (5). pp. 321-327. ISSN 1023–6244 (P), 1029–0362 (O) http://www.tandfonline.com/doi/abs/10.1080/10236244.2011.642505 DOI:10.1080/10236244.2011.642505
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) John, Akbar Khan Chowdhury, Ahmed Jalal Kasim, Zaleha Amstrong, Peter B. Yunus, Kamaruzzaman Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas)
description	Horseshoe crabs provide the important biomedical products, Limulus Amebocyte Lysate (LAL) and Tachypleus Amebocyte Lysate (TAL), which are produced by lysing the blood cells (amebocytes) using endotoxin-free distilled water. LAL/TAL is used to detect and quantify lipopolysaccharide (endotoxin), an important toxin of Gram-negative bacteria. Horseshoe crabs are the sole source for LAL/TAL and their fishery for the production of LAL/TAL is of concern to the sustained management of the species. This study investigated the mortality of the Malaysian horseshoe crab (Tachypleus gigas) related to blood extraction, with the aim of providing a basis for the sustainable management of the local fishery of this animal. We showed that the mortality rate of T. gigas due to the bleeding process is slightly higher than the values which has previously been reported for its conspecific, the American horseshoe crab (Limulus polyphemus). Overall mortalities of bled horseshoe crabs were 17.8% (male = 18.75%; female = 17.2%), while mortalities of unbled crabs were 3.5%. The quantification of the mortality due to the extent of bleeding commensurate with the commercial production of LAL/TAL provides a basis for the rational management of T. gigas in situations where harvest for LAL/TAL production is imposed on local populations of the animal.
format	Article
author	John, Akbar Khan Chowdhury, Ahmed Jalal Kasim, Zaleha Amstrong, Peter B. Yunus, Kamaruzzaman
author_facet	John, Akbar Khan Chowdhury, Ahmed Jalal Kasim, Zaleha Amstrong, Peter B. Yunus, Kamaruzzaman
author_sort	John, Akbar
title	Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas)
title_short	Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas)
title_full	Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas)
title_fullStr	Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas)
title_full_unstemmed	Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas)
title_sort	effects of blood extraction on the mortality of malaysian horseshoe crabs (tachypleus gigas)
publisher	Taylor & Francis
publishDate	2011
url	http://irep.iium.edu.my/22563/ http://irep.iium.edu.my/22563/ http://irep.iium.edu.my/22563/ http://irep.iium.edu.my/22563/1/10236244.2011.pdf
first_indexed	2023-09-18T20:34:19Z
last_indexed	2023-09-18T20:34:19Z
_version_	1777408944339156992

Effects of blood extraction on the mortality of Malaysian horseshoe crabs (Tachypleus gigas)

Similar Items