A real-time PCR technique in the identification of over-stunned slaughtered chicken
Chickens were water stunned before slaughtered was acceptable in Malaysia under certain condition prepared by MAJLIS FATWA and JAKIM. However, in some country like Brunei this method was not accepted at all. Even in Malaysia, it is unlawful when these chickens were stunned over the current limit...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/22646/ http://irep.iium.edu.my/22646/1/pp79.pdf |
| Summary: | Chickens were water stunned before slaughtered was acceptable in Malaysia under certain condition
prepared by MAJLIS FATWA and JAKIM. However, in some country like Brunei this method was not
accepted at all. Even in Malaysia, it is unlawful when these chickens were stunned over the current limit
that set by JAKIM. The previous study conducted identified a candidate biomarker that suitable to be used
in developing a detection method for over stunned chicken. A real-time PCR technique was applied as a
detection method in this study using our identified biomarker in developing a set of specific primers. This
study was conducted to observe the effectiveness of a detection method developed for identifying
chicken that were stunning over the current limit allowable by JAKIM. Results of this study showed that
by applying this detection method we were able to differentiate a chicken that was slaughtered according
to original Syara’i method compared to the one that over stunned prior to slaughtering and also able to
develop a result within 3 hours. |
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