Amino acids substitution to improve PhyFAUIA1 phytase activity for animal feed production
Phytate is largely unavailable to monogastric animal such as swine, poultry and fish, as they lack of sufficient endogenous enzymatic activity to hydrolyze phytate. The result is the elimination of precious nutrients that would be beneficial to their growth; furthermore they will excrete most o...
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Research Journal of Chemistry and Environment
2010
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| Online Access: | http://irep.iium.edu.my/2265/ http://irep.iium.edu.my/2265/ http://irep.iium.edu.my/2265/1/Pytase_Activity_328-333.pdf |
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iium-22652019-11-07T06:57:16Z http://irep.iium.edu.my/2265/ Amino acids substitution to improve PhyFAUIA1 phytase activity for animal feed production Samsudin , Nurhusna Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali Amid, Azura Q Science (General) TP248.13 Biotechnology Phytate is largely unavailable to monogastric animal such as swine, poultry and fish, as they lack of sufficient endogenous enzymatic activity to hydrolyze phytate. The result is the elimination of precious nutrients that would be beneficial to their growth; furthermore they will excrete most of the indigestive phytate which can contribute to phosphorus being over applied to the land. Phosphorus has a beneficial impact on vegetative growth on land as well as marine vegetation, causing an increased growth of weeds. This enhanced vegetation consumes large amounts of oxygen, resulting in the loss of aquatic life and ultimately contributes to water pollution and eutrophication of ground water and aquatic environment. Phytase, a type of histidine acid phosphatase hydrolyzes phytin phosphorus and when present in an animal's digestive tract, benefits the animal while reducing total phosphorus levels in manure. Computer modelling is used to identify and examine the active site of phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the binding strength before and after mutation. From the directive results of computational studies, point mutation was introduced by site directed mutagenesis using polymerase chain reaction (PCR). Mutagenesis is achieved by two step PCR procedure. Four primers were generated which two of them was design for carried the mutation at the point of interest which is complement to each other and the other two was design for unique restriction site. Several numbers of single, double as well as triple mutations has been introduced which will be further characterized to determine the activity of the enzyme as compared to the computational results. Research Journal of Chemistry and Environment 2010-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/2265/1/Pytase_Activity_328-333.pdf Samsudin , Nurhusna and Mohd. Salleh, Hamzah and Noorbatcha, Ibrahim Ali and Amid, Azura (2010) Amino acids substitution to improve PhyFAUIA1 phytase activity for animal feed production. Research Journal of Chemistry and Environment, Special (Issue). pp. 329-333. ISSN 0972-0626 http://www.chemenviron.net |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
Q Science (General) TP248.13 Biotechnology |
| spellingShingle |
Q Science (General) TP248.13 Biotechnology Samsudin , Nurhusna Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali Amid, Azura Amino acids substitution to improve PhyFAUIA1 phytase activity for animal feed production |
| description |
Phytate is largely unavailable to monogastric
animal such as swine, poultry and fish, as they lack of
sufficient endogenous enzymatic activity to hydrolyze
phytate. The result is the elimination of precious
nutrients that would be beneficial to their growth;
furthermore they will excrete most of the indigestive
phytate which can contribute to phosphorus being
over applied to the land. Phosphorus has a beneficial
impact on vegetative growth on land as well as
marine vegetation, causing an increased growth of
weeds.
This enhanced vegetation consumes large
amounts of oxygen, resulting in the loss of aquatic life
and ultimately contributes to water pollution and
eutrophication of ground water and aquatic
environment. Phytase, a type of histidine acid
phosphatase hydrolyzes phytin phosphorus and when
present in an animal's digestive tract, benefits the
animal while reducing total phosphorus levels in
manure. Computer modelling is used to identify and
examine the active site of phytase. The factors
influencing the ligand binding strength in the active
site is analyzed and computational site directed
mutagenesis experiments were carried out to evaluate
the effects of mutations on the binding strength before
and after mutation. From the directive results of computational studies, point mutation was introduced by site directed mutagenesis using polymerase chain reaction (PCR). Mutagenesis is achieved by two step PCR procedure.
Four primers were generated which two of them was
design for carried the mutation at the point of interest
which is complement to each other and the other two
was design for unique restriction site. Several
numbers of single, double as well as triple mutations
has been introduced which will be further
characterized to determine the activity of the enzyme
as compared to the computational results.
|
| format |
Article |
| author |
Samsudin , Nurhusna Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali Amid, Azura |
| author_facet |
Samsudin , Nurhusna Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali Amid, Azura |
| author_sort |
Samsudin , Nurhusna |
| title |
Amino acids substitution to improve PhyFAUIA1 phytase activity for animal feed production |
| title_short |
Amino acids substitution to improve PhyFAUIA1 phytase activity for animal feed production |
| title_full |
Amino acids substitution to improve PhyFAUIA1 phytase activity for animal feed production |
| title_fullStr |
Amino acids substitution to improve PhyFAUIA1 phytase activity for animal feed production |
| title_full_unstemmed |
Amino acids substitution to improve PhyFAUIA1 phytase activity for animal feed production |
| title_sort |
amino acids substitution to improve phyfauia1 phytase activity for animal feed production |
| publisher |
Research Journal of Chemistry and Environment |
| publishDate |
2010 |
| url |
http://irep.iium.edu.my/2265/ http://irep.iium.edu.my/2265/ http://irep.iium.edu.my/2265/1/Pytase_Activity_328-333.pdf |
| first_indexed |
2023-09-18T20:09:48Z |
| last_indexed |
2023-09-18T20:09:48Z |
| _version_ |
1777407401664708608 |