Characterization of purified cellulase from fermentation of sewage sludge

The increasing volume of sewage sludge as well as the municipal and agricultural waste produced and the total managing cost every year has been one of the major environmental issues in Malaysia. The production of cellulase from domestic sewage sludge could possibly bring an economic importance in...

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Main Authors: Mukhtaruddin, Muna Tasnim, Alam, Md Zahangir, Mohd. Salleh, Hamzah
Format: Article
Language:English
Published: INSI Publications 2012
Subjects:
Online Access:http://irep.iium.edu.my/23412/
http://irep.iium.edu.my/23412/
http://irep.iium.edu.my/23412/1/muna_tasnim_et_al.pdf
id iium-23412
recordtype eprints
spelling iium-234122017-08-10T01:52:29Z http://irep.iium.edu.my/23412/ Characterization of purified cellulase from fermentation of sewage sludge Mukhtaruddin, Muna Tasnim Alam, Md Zahangir Mohd. Salleh, Hamzah T175 Industrial research. Research and development TA170 Environmental engineering TP248.13 Biotechnology The increasing volume of sewage sludge as well as the municipal and agricultural waste produced and the total managing cost every year has been one of the major environmental issues in Malaysia. The production of cellulase from domestic sewage sludge could possibly bring an economic importance in certain industries as well as a key to solve the problem of environmental pollution and also in developing and utilizing renewable energy sources by applying the enzyme on the cellulosic materials. This study focuses on the characterization of cellulases produced from fermentation broth of sewage sludge. Using the cellulase enzyme with the activity of 45 U/ml, few tests have been carried out to characterize and checked the stability of the enzyme. The tests were pH stability, temperature stability as well as incubation time for the enzyme. Characterization of the enzyme was likely to help develop efficient application of the enzyme in industrial scale. 50oC was found to be the optimum temperature for the stability of the enzyme. Besides, pH 3 gave the highest enzyme activity and also 48 hours turn to be the optimum incubation time. INSI Publications 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/23412/1/muna_tasnim_et_al.pdf Mukhtaruddin, Muna Tasnim and Alam, Md Zahangir and Mohd. Salleh, Hamzah (2012) Characterization of purified cellulase from fermentation of sewage sludge. Australian Journal of Basic and Applied Sciences, 6 (1). pp. 74-78. ISSN 1991-8178 http://www.ajbasweb.com/ajbas/2012/January/74-78.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic T175 Industrial research. Research and development
TA170 Environmental engineering
TP248.13 Biotechnology
spellingShingle T175 Industrial research. Research and development
TA170 Environmental engineering
TP248.13 Biotechnology
Mukhtaruddin, Muna Tasnim
Alam, Md Zahangir
Mohd. Salleh, Hamzah
Characterization of purified cellulase from fermentation of sewage sludge
description The increasing volume of sewage sludge as well as the municipal and agricultural waste produced and the total managing cost every year has been one of the major environmental issues in Malaysia. The production of cellulase from domestic sewage sludge could possibly bring an economic importance in certain industries as well as a key to solve the problem of environmental pollution and also in developing and utilizing renewable energy sources by applying the enzyme on the cellulosic materials. This study focuses on the characterization of cellulases produced from fermentation broth of sewage sludge. Using the cellulase enzyme with the activity of 45 U/ml, few tests have been carried out to characterize and checked the stability of the enzyme. The tests were pH stability, temperature stability as well as incubation time for the enzyme. Characterization of the enzyme was likely to help develop efficient application of the enzyme in industrial scale. 50oC was found to be the optimum temperature for the stability of the enzyme. Besides, pH 3 gave the highest enzyme activity and also 48 hours turn to be the optimum incubation time.
format Article
author Mukhtaruddin, Muna Tasnim
Alam, Md Zahangir
Mohd. Salleh, Hamzah
author_facet Mukhtaruddin, Muna Tasnim
Alam, Md Zahangir
Mohd. Salleh, Hamzah
author_sort Mukhtaruddin, Muna Tasnim
title Characterization of purified cellulase from fermentation of sewage sludge
title_short Characterization of purified cellulase from fermentation of sewage sludge
title_full Characterization of purified cellulase from fermentation of sewage sludge
title_fullStr Characterization of purified cellulase from fermentation of sewage sludge
title_full_unstemmed Characterization of purified cellulase from fermentation of sewage sludge
title_sort characterization of purified cellulase from fermentation of sewage sludge
publisher INSI Publications
publishDate 2012
url http://irep.iium.edu.my/23412/
http://irep.iium.edu.my/23412/
http://irep.iium.edu.my/23412/1/muna_tasnim_et_al.pdf
first_indexed 2023-09-18T20:35:26Z
last_indexed 2023-09-18T20:35:26Z
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