Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surf...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2012
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/1/Bala_final_2191-0855-2-12.pdf |
| Summary: | Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli,
BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and
post-induction period). The optimized conditions by response surface methodology using face centered central
composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of
R2 (0.989) showed that the quadratic model used for the prediction was highly significant (p < 0.05). Under the
optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave
bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively
developed cultivation conditions for better production of recombinant bromelain in shake flask culture. |
|---|