Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology

Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surf...

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Main Authors:	Muntari, Bala, Amid, Azura, Jami, Mohammed Saedi, Mohd. Salleh, Hamzah, Mel, Maizirwan
Format:	Article
Language:	English
Published:	Springer 2012
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/1/Bala_final_2191-0855-2-12.pdf

id	iium-23637
recordtype	eprints
spelling	iium-236372014-07-17T01:37:06Z http://irep.iium.edu.my/23637/ Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology Muntari, Bala Amid, Azura Jami, Mohammed Saedi Mohd. Salleh, Hamzah Mel, Maizirwan Q Science (General) QR Microbiology Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surface methodology using face centered central composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of R2 (0.989) showed that the quadratic model used for the prediction was highly significant (p < 0.05). Under the optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively developed cultivation conditions for better production of recombinant bromelain in shake flask culture. Springer 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/23637/1/Bala_final_2191-0855-2-12.pdf Muntari, Bala and Amid, Azura and Jami, Mohammed Saedi and Mohd. Salleh, Hamzah and Mel, Maizirwan (2012) Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology. AMB Express, 2 (12). pp. 1-9. ISSN 2191-0855 http://www.amb-express.com/content/2/1/12 10.1186/2191-0855-2-12
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Muntari, Bala Amid, Azura Jami, Mohammed Saedi Mohd. Salleh, Hamzah Mel, Maizirwan Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
description	Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surface methodology using face centered central composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of R2 (0.989) showed that the quadratic model used for the prediction was highly significant (p < 0.05). Under the optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively developed cultivation conditions for better production of recombinant bromelain in shake flask culture.
format	Article
author	Muntari, Bala Amid, Azura Jami, Mohammed Saedi Mohd. Salleh, Hamzah Mel, Maizirwan
author_facet	Muntari, Bala Amid, Azura Jami, Mohammed Saedi Mohd. Salleh, Hamzah Mel, Maizirwan
author_sort	Muntari, Bala
title	Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
title_short	Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
title_full	Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
title_fullStr	Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
title_full_unstemmed	Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
title_sort	recombinant bromelain production in escherichia coli: process optimization in shake flask culture by response surface methodology
publisher	Springer
publishDate	2012
url	http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/1/Bala_final_2191-0855-2-12.pdf
first_indexed	2023-09-18T20:35:43Z
last_indexed	2023-09-18T20:35:43Z
_version_	1777409032430026752

Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology

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