Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence

An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) w...

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Main Authors:	John, Akbar, Khan Chowdhury, Ahmed Jalal, Yunus, Kamaruzzaman, Kasim, Zaleha, Kumar, C. Prasanna
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	QL Zoology
Online Access:	http://irep.iium.edu.my/2404/ http://irep.iium.edu.my/2404/ http://irep.iium.edu.my/2404/2/ICPE%25202010.pdf

id	iium-2404
recordtype	eprints
spelling	iium-24042017-12-06T04:30:46Z http://irep.iium.edu.my/2404/ Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence John, Akbar Khan Chowdhury, Ahmed Jalal Yunus, Kamaruzzaman Kasim, Zaleha Kumar, C. Prasanna QL Zoology An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) which include horseshoe crabs, beetles, common crabs and scorpion sequences. Constructed phylogram showed beetles are closely related with horseshoe crabs than common crabs. Scorpion spp were distantly related to xiphosurans. Phylogram and observed genetic distance (GD) date were also revealed that Limulus polyphemus was closely related with Tachypleus tridentatus than with T.gigas. Carcinoscorpius rotundicauda was distantly related with L.polyphemus. The observed mean Genetic Distance (GD) value was higher in 3rd codon position in all the selected group of organisms. Among the horseshoe crabs high GC content was observed in L.polyphemus (38.32%) and lowest was observed in T.tridentatus (32.35%). We conclude that COI sequencing (barcoding) could be used in identifying and delineating evolutionary relatedness with closely related species 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/2404/2/ICPE%25202010.pdf John, Akbar and Khan Chowdhury, Ahmed Jalal and Yunus, Kamaruzzaman and Kasim, Zaleha and Kumar, C. Prasanna (2010) Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence. In: 4th International Conference on Postgraduate Education (ICPE 4). , 26 - 28 November 2010, Kuala Lumpur, Malaysia. http://pkukmweb.ukm.my/~icpe4/pdf/ICPE%202010.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QL Zoology
spellingShingle	QL Zoology John, Akbar Khan Chowdhury, Ahmed Jalal Yunus, Kamaruzzaman Kasim, Zaleha Kumar, C. Prasanna Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence
description	An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) which include horseshoe crabs, beetles, common crabs and scorpion sequences. Constructed phylogram showed beetles are closely related with horseshoe crabs than common crabs. Scorpion spp were distantly related to xiphosurans. Phylogram and observed genetic distance (GD) date were also revealed that Limulus polyphemus was closely related with Tachypleus tridentatus than with T.gigas. Carcinoscorpius rotundicauda was distantly related with L.polyphemus. The observed mean Genetic Distance (GD) value was higher in 3rd codon position in all the selected group of organisms. Among the horseshoe crabs high GC content was observed in L.polyphemus (38.32%) and lowest was observed in T.tridentatus (32.35%). We conclude that COI sequencing (barcoding) could be used in identifying and delineating evolutionary relatedness with closely related species
format	Conference or Workshop Item
author	John, Akbar Khan Chowdhury, Ahmed Jalal Yunus, Kamaruzzaman Kasim, Zaleha Kumar, C. Prasanna
author_facet	John, Akbar Khan Chowdhury, Ahmed Jalal Yunus, Kamaruzzaman Kasim, Zaleha Kumar, C. Prasanna
author_sort	John, Akbar
title	Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence
title_short	Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence
title_full	Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence
title_fullStr	Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence
title_full_unstemmed	Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence
title_sort	molecular phylogeny of horseshoe crab using mitochondrial cox1 gene as a benchmark sequence
publishDate	2010
url	http://irep.iium.edu.my/2404/ http://irep.iium.edu.my/2404/ http://irep.iium.edu.my/2404/2/ICPE%25202010.pdf
first_indexed	2023-09-18T20:10:00Z
last_indexed	2023-09-18T20:10:00Z
_version_	1777407413844967424

Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence

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