Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh

Characterization and quantification of carotenoid compound is complicated, costly and timeconsuming. The accuracy and reliability of the data depend solely on the standard and to High Performance Liquid Chromatography (HPLC) analysis but the major constraint is to acquire and to maintain the pure...

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Main Authors:	Shahidan, Norshazila, Jaswir, Irwandi, Othman, Rashidi, Hashim, Yumi Zuhanis Has-Yun
Format:	Article
Language:	English
Published:	Faculty of Food Science and Technology, Universiti Putra Malaysia 2012
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/24207/ http://irep.iium.edu.my/24207/ http://irep.iium.edu.my/24207/1/Scheme_of_obtaining_%CE%B2-carotene_standard.pdf

id	iium-24207
recordtype	eprints
spelling	iium-242072012-06-11T07:01:42Z http://irep.iium.edu.my/24207/ Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh Shahidan, Norshazila Jaswir, Irwandi Othman, Rashidi Hashim, Yumi Zuhanis Has-Yun Q Science (General) Characterization and quantification of carotenoid compound is complicated, costly and timeconsuming. The accuracy and reliability of the data depend solely on the standard and to High Performance Liquid Chromatography (HPLC) analysis but the major constraint is to acquire and to maintain the pure standards. Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus, the purpose of this study is to establish an analytical method for isolating β-carotene by using open column chromatography (OCC) from pumpkin (Cucurbita moschata) to be used as one of the carotenoid standards for determination of total and individual carotenoid. Pumpkin with orange flesh has been chosen due to the non-seasonal nature and its availability all year-round. This study demonstrated that the purity of β-carotene standard; determined by HPLC was ranged from 92.21 to 97.95%. The standard curves with five different concentrations of β-carotene extract from pumpkins in triplicate were constructed by plotting the peak area against the concentration. The coefficient of correlation was 0.9936. Therefore, this study established that pumpkin can be a reliable source of beta-carotene standard as it is cheap and commonly available throughout the year. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/24207/1/Scheme_of_obtaining_%CE%B2-carotene_standard.pdf Shahidan, Norshazila and Jaswir, Irwandi and Othman, Rashidi and Hashim, Yumi Zuhanis Has-Yun (2012) Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh. International Food Research Journal, 19 (2). pp. 531-535. ISSN 19854668 (P), 22317546 (O) http://www.ifrj.upm.edu.my/19%20(02)%202012/(23)IFRJ-2012%20Irwandi.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Shahidan, Norshazila Jaswir, Irwandi Othman, Rashidi Hashim, Yumi Zuhanis Has-Yun Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
description	Characterization and quantification of carotenoid compound is complicated, costly and timeconsuming. The accuracy and reliability of the data depend solely on the standard and to High Performance Liquid Chromatography (HPLC) analysis but the major constraint is to acquire and to maintain the pure standards. Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus, the purpose of this study is to establish an analytical method for isolating β-carotene by using open column chromatography (OCC) from pumpkin (Cucurbita moschata) to be used as one of the carotenoid standards for determination of total and individual carotenoid. Pumpkin with orange flesh has been chosen due to the non-seasonal nature and its availability all year-round. This study demonstrated that the purity of β-carotene standard; determined by HPLC was ranged from 92.21 to 97.95%. The standard curves with five different concentrations of β-carotene extract from pumpkins in triplicate were constructed by plotting the peak area against the concentration. The coefficient of correlation was 0.9936. Therefore, this study established that pumpkin can be a reliable source of beta-carotene standard as it is cheap and commonly available throughout the year.
format	Article
author	Shahidan, Norshazila Jaswir, Irwandi Othman, Rashidi Hashim, Yumi Zuhanis Has-Yun
author_facet	Shahidan, Norshazila Jaswir, Irwandi Othman, Rashidi Hashim, Yumi Zuhanis Has-Yun
author_sort	Shahidan, Norshazila
title	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_short	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_full	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_fullStr	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_full_unstemmed	Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
title_sort	scheme of obtaining ß-carotene standard from pumpkin (cucurbita moschata) flesh
publisher	Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate	2012
url	http://irep.iium.edu.my/24207/ http://irep.iium.edu.my/24207/ http://irep.iium.edu.my/24207/1/Scheme_of_obtaining_%CE%B2-carotene_standard.pdf
first_indexed	2023-09-18T20:36:22Z
last_indexed	2023-09-18T20:36:22Z
_version_	1777409072513941504

Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh

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