Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro

Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro

Context: Andrographis paniculata Nees. (Acanthaceae) is an annual herbaceous plant widely cultivated in southern Asia, China, and Europe. It is used in the treatment of skin infections in India, China, and Malaysia by folk medicine practitioners. Objective: Antifungal activity of the whole plant e...

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Main Authors: Sule, Abubakar, Ahmed, Qamar Uddin, Latip, Jalifah, abd Samah, Othman, Omar, Muhammad Nor, Umar, Abdulrashid, Dogarai, Bashar Bello S.
Format: Article
Language:English
Published: Informa Healthcare 2012
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/24434/
http://irep.iium.edu.my/24434/
http://irep.iium.edu.my/24434/1/Antifungal_activity_of_Andrographis_paniculata_extracts.pdf
id iium-24434
recordtype eprints
spelling iium-244342012-07-02T01:36:31Z http://irep.iium.edu.my/24434/ Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro Sule, Abubakar Ahmed, Qamar Uddin Latip, Jalifah abd Samah, Othman Omar, Muhammad Nor Umar, Abdulrashid Dogarai, Bashar Bello S. QD Chemistry Context: Andrographis paniculata Nees. (Acanthaceae) is an annual herbaceous plant widely cultivated in southern Asia, China, and Europe. It is used in the treatment of skin infections in India, China, and Malaysia by folk medicine practitioners. Objective: Antifungal activity of the whole plant extracts and isolation of active principles from A. paniculata were investigated. Materials and methods: Dichloromethane (DCM) and methanol (MEOH) extracts of A. paniculata whole plant were screened for their antifungal potential using broth microdilution method in vitro against seven pathogenic fungal species responsible for skin infections. Active principles were detected through bioguided assays and isolated using chromatography techniques. Structures of compounds were elucidated through spectroscopy techniques and comparisons were made with previously reported data for similar compounds. Results: DCM extract revealed lowest minimum inhibitory concentration (MIC) value (100 μg/mL) against Microsporum canis, Candida albicans, and Candida tropicalis, whereas MEOH extract revealed lowest MIC (150 μg/mL) against C. tropicalis and Aspergillus niger. DCM extract showed lowest minimum fungicidal concentration (MFC) value (250 μg/mL) against M. canis, C. albicans, C. tropicalis and A. niger, whereas MEOH extract showed lowest MFC (250 μg/mL) against Trichophyton mentagrophytes, Trichophyton rubrum, M. canis, C. albicans, C. tropicalis and A. niger. Bioassay guided isolation from DCM and MEOH extract afforded 3-O-β-d-glucosyl-14-deoxyandrographiside, 14-deoxyandrographolide, and 14-deoxy-11,12-didehydroandrographolide as antifungal compounds. The lowest MIC (50 μg/mL) and MFC (50 μg/mL) was exerted by 14-deoxyandrographolide on M. canis. Discussion and conclusion: This is first report on the isolation of antifungal substances through bioassay-guided assay from A. paniculata. Our finding justifies the use of A. paniculata in folk medicines for the treatment of fungal skin infections. Informa Healthcare 2012-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/24434/1/Antifungal_activity_of_Andrographis_paniculata_extracts.pdf Sule, Abubakar and Ahmed, Qamar Uddin and Latip, Jalifah and abd Samah, Othman and Omar, Muhammad Nor and Umar, Abdulrashid and Dogarai, Bashar Bello S. (2012) Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro. Pharmaceutical Biology, 50 (7). pp. 850-856. ISSN 1388-0209 http://informahealthcare.com/loi/phb
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Sule, Abubakar
Ahmed, Qamar Uddin
Latip, Jalifah
abd Samah, Othman
Omar, Muhammad Nor
Umar, Abdulrashid
Dogarai, Bashar Bello S.
Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro
description Context: Andrographis paniculata Nees. (Acanthaceae) is an annual herbaceous plant widely cultivated in southern Asia, China, and Europe. It is used in the treatment of skin infections in India, China, and Malaysia by folk medicine practitioners. Objective: Antifungal activity of the whole plant extracts and isolation of active principles from A. paniculata were investigated. Materials and methods: Dichloromethane (DCM) and methanol (MEOH) extracts of A. paniculata whole plant were screened for their antifungal potential using broth microdilution method in vitro against seven pathogenic fungal species responsible for skin infections. Active principles were detected through bioguided assays and isolated using chromatography techniques. Structures of compounds were elucidated through spectroscopy techniques and comparisons were made with previously reported data for similar compounds. Results: DCM extract revealed lowest minimum inhibitory concentration (MIC) value (100 μg/mL) against Microsporum canis, Candida albicans, and Candida tropicalis, whereas MEOH extract revealed lowest MIC (150 μg/mL) against C. tropicalis and Aspergillus niger. DCM extract showed lowest minimum fungicidal concentration (MFC) value (250 μg/mL) against M. canis, C. albicans, C. tropicalis and A. niger, whereas MEOH extract showed lowest MFC (250 μg/mL) against Trichophyton mentagrophytes, Trichophyton rubrum, M. canis, C. albicans, C. tropicalis and A. niger. Bioassay guided isolation from DCM and MEOH extract afforded 3-O-β-d-glucosyl-14-deoxyandrographiside, 14-deoxyandrographolide, and 14-deoxy-11,12-didehydroandrographolide as antifungal compounds. The lowest MIC (50 μg/mL) and MFC (50 μg/mL) was exerted by 14-deoxyandrographolide on M. canis. Discussion and conclusion: This is first report on the isolation of antifungal substances through bioassay-guided assay from A. paniculata. Our finding justifies the use of A. paniculata in folk medicines for the treatment of fungal skin infections.
format Article
author Sule, Abubakar
Ahmed, Qamar Uddin
Latip, Jalifah
abd Samah, Othman
Omar, Muhammad Nor
Umar, Abdulrashid
Dogarai, Bashar Bello S.
author_facet Sule, Abubakar
Ahmed, Qamar Uddin
Latip, Jalifah
abd Samah, Othman
Omar, Muhammad Nor
Umar, Abdulrashid
Dogarai, Bashar Bello S.
author_sort Sule, Abubakar
title Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro
title_short Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro
title_full Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro
title_fullStr Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro
title_full_unstemmed Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro
title_sort antifungal activity of andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro
publisher Informa Healthcare
publishDate 2012
url http://irep.iium.edu.my/24434/
http://irep.iium.edu.my/24434/
http://irep.iium.edu.my/24434/1/Antifungal_activity_of_Andrographis_paniculata_extracts.pdf
first_indexed 2023-09-18T20:36:38Z
last_indexed 2023-09-18T20:36:38Z
_version_ 1777409089109753856

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