Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare
Serum; a clear portion of blood obtained after removing cells, platelets and clotting factors is a universal supplement commonly used in media for mammalian cell culture system. Serum contains amino acids, proteins, growth factors, hormones, vitamins, inorganic substances, nutrients and metabolite...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
American-Eurasian Network for Scientific Information (AENSI)
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/24535/ http://irep.iium.edu.my/24535/ http://irep.iium.edu.my/24535/1/Serum_in_mammalian_cell_culture-_weighing_the_challenges_of.pdf http://irep.iium.edu.my/24535/4/24535_Serum%20in%20mammalian%20cell%20culture_SCOPUS.pdf |
| Summary: | Serum; a clear portion of blood obtained after removing cells, platelets and clotting factors is a universal
supplement commonly used in media for mammalian cell culture system. Serum contains amino acids, proteins,
growth factors, hormones, vitamins, inorganic substances, nutrients and metabolites; which promote and sustain
cell growth as well as provide buffering condition and protection to cells. Serum, from various animal sources
such as bovine and porcine can be obtained commercially from manufacturers. Although serum has long been
used successfully in mammalian cell culture system, recent trend is moving towards serum alternatives and
serum-free media. This is due to many factors including the ethical and animal welfare issues in serum
processing and production. Further, large scale production of bio-products using mammalian cell culture system
prefers defined media which offers reproducibility and ease of downstream purification. Nevertheless,
development of serum-free media is tedious and somewhat involve high cost with inherent problem of low
growth rate as compared to serum supplemented media. This review is set to explore the many facets of serum
and serum-free media in mammalian cell culture bioprocessing. |
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