Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma

Introduction: Synovial Sarcoma is a rare cancer and account for 5-10% of adult soft tissue sarcomas. The tumour exhibits unspecified histogenesis composed primarily of spindle cells with variable epithelial components. Despite establishment of some immunohistochemistry staining, making a definitive...

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Main Authors:	Effendy Goon, Danial, Harun, Norra, Muhammad, Siti Aesah @ Naznin, A.Talib, Norlelawati
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/24612/ http://irep.iium.edu.my/24612/1/Abstract_CPath2012.pdf

id	iium-24612
recordtype	eprints
spelling	iium-246122012-10-19T06:33:28Z http://irep.iium.edu.my/24612/ Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma Effendy Goon, Danial Harun, Norra Muhammad, Siti Aesah @ Naznin A.Talib, Norlelawati RB Pathology Introduction: Synovial Sarcoma is a rare cancer and account for 5-10% of adult soft tissue sarcomas. The tumour exhibits unspecified histogenesis composed primarily of spindle cells with variable epithelial components. Despite establishment of some immunohistochemistry staining, making a definitive diagnosis of synovial sarcoma remains a challenging task. This is due to the histo-morphology and immunophenotypes similarities of this tumour to other types of soft tissue sarcoma. Objective: The current study aims to apply a molecular method for detection of SYT-SSX fusion transcript, a common molecular defect (>90% of the cases) in Synovial Sarcoma irrespective of the histologic subtypes. Method: Paraffin-embedded fixed-tissue (PEFT) blocks of 3 confirmed and 15 possible cases of Synovial Sarcoma were retrieved from Department of Pathology, Tengku Ampuan-Afzan Hospital, Kuantan and subjected to RNA purification using the standard spin column protocol. A one step direct RT-PCR was performed using SYT-SSX and PBGD primer sets for detection of SYT-SSX fusion gene and the reference gene PBGD respectively. Results: Our preliminary molecular analyses showed positive SYT-SSX fusion transcript in all 3 confirmed cases and 5 possible cases of synovial sarcoma. Further analysis is still on going for the remaining samples. Conclusion: Molecular detection of SYT-SSX fusion-transcript is useful in establishing the diagnosis of Synovial Sarcoma. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/24612/1/Abstract_CPath2012.pdf Effendy Goon, Danial and Harun, Norra and Muhammad, Siti Aesah @ Naznin and A.Talib, Norlelawati (2012) Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma. In: 11th Annual Scientific Meeting, College of Pathologist Malaysia, 8-10 June 2012, Crowne Plaza Mutiara, Kuala Lumpur.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology
spellingShingle	RB Pathology Effendy Goon, Danial Harun, Norra Muhammad, Siti Aesah @ Naznin A.Talib, Norlelawati Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
description	Introduction: Synovial Sarcoma is a rare cancer and account for 5-10% of adult soft tissue sarcomas. The tumour exhibits unspecified histogenesis composed primarily of spindle cells with variable epithelial components. Despite establishment of some immunohistochemistry staining, making a definitive diagnosis of synovial sarcoma remains a challenging task. This is due to the histo-morphology and immunophenotypes similarities of this tumour to other types of soft tissue sarcoma. Objective: The current study aims to apply a molecular method for detection of SYT-SSX fusion transcript, a common molecular defect (>90% of the cases) in Synovial Sarcoma irrespective of the histologic subtypes. Method: Paraffin-embedded fixed-tissue (PEFT) blocks of 3 confirmed and 15 possible cases of Synovial Sarcoma were retrieved from Department of Pathology, Tengku Ampuan-Afzan Hospital, Kuantan and subjected to RNA purification using the standard spin column protocol. A one step direct RT-PCR was performed using SYT-SSX and PBGD primer sets for detection of SYT-SSX fusion gene and the reference gene PBGD respectively. Results: Our preliminary molecular analyses showed positive SYT-SSX fusion transcript in all 3 confirmed cases and 5 possible cases of synovial sarcoma. Further analysis is still on going for the remaining samples. Conclusion: Molecular detection of SYT-SSX fusion-transcript is useful in establishing the diagnosis of Synovial Sarcoma.
format	Conference or Workshop Item
author	Effendy Goon, Danial Harun, Norra Muhammad, Siti Aesah @ Naznin A.Talib, Norlelawati
author_facet	Effendy Goon, Danial Harun, Norra Muhammad, Siti Aesah @ Naznin A.Talib, Norlelawati
author_sort	Effendy Goon, Danial
title	Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_short	Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_full	Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_fullStr	Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_full_unstemmed	Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
title_sort	preliminary analyses on detection of syt-ssx fusion-transcripts in synovial sarcoma
publishDate	2012
url	http://irep.iium.edu.my/24612/ http://irep.iium.edu.my/24612/1/Abstract_CPath2012.pdf
first_indexed	2023-09-18T20:36:53Z
last_indexed	2023-09-18T20:36:53Z
_version_	1777409105508433920

Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma

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