The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia

Objective : To determine the proliferative activity of cervical intraepithelial lesions to assist in the diagnosis and treatment of these lesions through the use of silver stained nucleolar organizer region (Ag +– NOR). Nucleolar organizer regions (NOR) are loops of ribosomal DNA (rDNA) occurring i...

Full description

Bibliographic Details
Main Authors:	Faisal, Ghasak Ghazi, Hussein, Alla'a Ghani
Format:	Conference or Workshop Item
Language:	English English
Published:	2012
Subjects:	RB Pathology
Online Access:	http://irep.iium.edu.my/24666/ http://irep.iium.edu.my/24666/ http://irep.iium.edu.my/24666/1/abstract_for_10th_international_congress_of_RCOG-dr_ghasak.pdf http://irep.iium.edu.my/24666/4/Program_book_evidence.pdf

id	iium-24666
recordtype	eprints
spelling	iium-246662012-07-16T01:29:36Z http://irep.iium.edu.my/24666/ The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia Faisal, Ghasak Ghazi Hussein, Alla'a Ghani RB Pathology Objective : To determine the proliferative activity of cervical intraepithelial lesions to assist in the diagnosis and treatment of these lesions through the use of silver stained nucleolar organizer region (Ag +– NOR). Nucleolar organizer regions (NOR) are loops of ribosomal DNA (rDNA) occurring in the nuclei of cells.Cell proliferation under normal physiological conditions is accompanied by increased ribosome biogenesis .The amount of Ag-NOR proteins present during interphase is routinely used to evaluate nucleolar activity and cell proliferation. Thus quantification of Ag-NOR proteins is applied to characterize human cancer cell proliferation ; high levels of Ag-NOR proteins indicate a high rate of proliferation . Methodology : Fifty cervical smears ,representing different cervical lesions ranging from inflammatory lesions (reactive)to LSIL (low grade intraepithelial lesion), HSIL (high grade intraepithelial lesion) and squamous cell carcinoma , were collected and stained using the Standardized Ag-NOR staining technique . After staining, representative areas in each slide were selected and NORs were counted. Results: The results showed that the lowest mean Ag-NOR count was observed in the benign reactive group (control) =2.07, There is a progressive increase in mean Ag-NOR count with increasing severity of cervical lesion, The highest mean Ag-NOR count belonged to squamous cell carcinoma group (9.02). The results also showed a statistically significant difference among the Ag-NOR counts of the different studied categories Conclusion: we can conclude that counts of Ag-NOR could be of great diagnostic value for the cytopathologists and gynecologists in conjunction with routine pap stain to evaluate cellular proliferative activity of the lesion to plan for treatment and for follow up of patients after treatment to detect regression in the severity of the lesion. 2012-06-05 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/24666/1/abstract_for_10th_international_congress_of_RCOG-dr_ghasak.pdf application/pdf en http://irep.iium.edu.my/24666/4/Program_book_evidence.pdf Faisal, Ghasak Ghazi and Hussein, Alla'a Ghani (2012) The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia. In: The 10th RCOG International scientific congress, 6-8 June 2012, Kuching, Sarawak. (Unpublished) http://www.rcog2012.com/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RB Pathology
spellingShingle	RB Pathology Faisal, Ghasak Ghazi Hussein, Alla'a Ghani The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia
description	Objective : To determine the proliferative activity of cervical intraepithelial lesions to assist in the diagnosis and treatment of these lesions through the use of silver stained nucleolar organizer region (Ag +– NOR). Nucleolar organizer regions (NOR) are loops of ribosomal DNA (rDNA) occurring in the nuclei of cells.Cell proliferation under normal physiological conditions is accompanied by increased ribosome biogenesis .The amount of Ag-NOR proteins present during interphase is routinely used to evaluate nucleolar activity and cell proliferation. Thus quantification of Ag-NOR proteins is applied to characterize human cancer cell proliferation ; high levels of Ag-NOR proteins indicate a high rate of proliferation . Methodology : Fifty cervical smears ,representing different cervical lesions ranging from inflammatory lesions (reactive)to LSIL (low grade intraepithelial lesion), HSIL (high grade intraepithelial lesion) and squamous cell carcinoma , were collected and stained using the Standardized Ag-NOR staining technique . After staining, representative areas in each slide were selected and NORs were counted. Results: The results showed that the lowest mean Ag-NOR count was observed in the benign reactive group (control) =2.07, There is a progressive increase in mean Ag-NOR count with increasing severity of cervical lesion, The highest mean Ag-NOR count belonged to squamous cell carcinoma group (9.02). The results also showed a statistically significant difference among the Ag-NOR counts of the different studied categories Conclusion: we can conclude that counts of Ag-NOR could be of great diagnostic value for the cytopathologists and gynecologists in conjunction with routine pap stain to evaluate cellular proliferative activity of the lesion to plan for treatment and for follow up of patients after treatment to detect regression in the severity of the lesion.
format	Conference or Workshop Item
author	Faisal, Ghasak Ghazi Hussein, Alla'a Ghani
author_facet	Faisal, Ghasak Ghazi Hussein, Alla'a Ghani
author_sort	Faisal, Ghasak Ghazi
title	The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia
title_short	The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia
title_full	The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia
title_fullStr	The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia
title_full_unstemmed	The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia
title_sort	use of ag-nor stain to determine the proliferative activity of cervical intraepithelial neoplasia
publishDate	2012
url	http://irep.iium.edu.my/24666/ http://irep.iium.edu.my/24666/ http://irep.iium.edu.my/24666/1/abstract_for_10th_international_congress_of_RCOG-dr_ghasak.pdf http://irep.iium.edu.my/24666/4/Program_book_evidence.pdf
first_indexed	2023-09-18T20:36:58Z
last_indexed	2023-09-18T20:36:58Z
_version_	1777409110607659008

The use of Ag-NOR stain to determine the proliferative activity of cervical intraepithelial neoplasia

Similar Items