Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka

Three local species of sea cucumbers; Holothuria edulis Lesson, H. scabra Jaeger and Stichopus horrens Selenka were studied for their body walls protein profiles using sodium dodecyl sulphate polyacylamide gel electrophoresis (SDS-PAGE). Their body walls were subjected to extraction process using...

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Main Authors: Hashim, Ridzwan, Z., Farah Hanis, Mohd Daud, Jamaluddin, Althunibat, Osama Yousef
Format: Article
Language:English
Published: EuroJournals Publishing, Inc. 2012
Subjects:
Online Access:http://irep.iium.edu.my/25253/
http://irep.iium.edu.my/25253/
http://irep.iium.edu.my/25253/1/PROTEIN_PROFILES.pdf
id iium-25253
recordtype eprints
spelling iium-252532012-11-09T05:12:47Z http://irep.iium.edu.my/25253/ Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka Hashim, Ridzwan Z., Farah Hanis Mohd Daud, Jamaluddin Althunibat, Osama Yousef QK Botany Three local species of sea cucumbers; Holothuria edulis Lesson, H. scabra Jaeger and Stichopus horrens Selenka were studied for their body walls protein profiles using sodium dodecyl sulphate polyacylamide gel electrophoresis (SDS-PAGE). Their body walls were subjected to extraction process using lysis and resuspension buffers. The protein concentration was based on bovine serum albumin as standard. Analysis of the protein extract of the three species of sea cucumber revealed at least 11 type of proteins with molecular weight ranging from 20 kDa – 125 kDa. As for the protein profiles, significant difference in the protein binding patterns were noticed. The results showed the molecular weight of protein varied among species; 20 – 54 kDa for H. edulis and 54 – 125 kDa for H. scabra. However, protein fraction for S. horrens failed to be detected using this technique. EuroJournals Publishing, Inc. 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/25253/1/PROTEIN_PROFILES.pdf Hashim, Ridzwan and Z., Farah Hanis and Mohd Daud, Jamaluddin and Althunibat, Osama Yousef (2012) Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka. European Journal of Scientific Research, 75 (2). pp. 255-261. ISSN 1450-216X, 1450-202X http://www.europeanjournalofscientificresearch.com/ISSUES/EJSR_75_2.htm
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QK Botany
spellingShingle QK Botany
Hashim, Ridzwan
Z., Farah Hanis
Mohd Daud, Jamaluddin
Althunibat, Osama Yousef
Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka
description Three local species of sea cucumbers; Holothuria edulis Lesson, H. scabra Jaeger and Stichopus horrens Selenka were studied for their body walls protein profiles using sodium dodecyl sulphate polyacylamide gel electrophoresis (SDS-PAGE). Their body walls were subjected to extraction process using lysis and resuspension buffers. The protein concentration was based on bovine serum albumin as standard. Analysis of the protein extract of the three species of sea cucumber revealed at least 11 type of proteins with molecular weight ranging from 20 kDa – 125 kDa. As for the protein profiles, significant difference in the protein binding patterns were noticed. The results showed the molecular weight of protein varied among species; 20 – 54 kDa for H. edulis and 54 – 125 kDa for H. scabra. However, protein fraction for S. horrens failed to be detected using this technique.
format Article
author Hashim, Ridzwan
Z., Farah Hanis
Mohd Daud, Jamaluddin
Althunibat, Osama Yousef
author_facet Hashim, Ridzwan
Z., Farah Hanis
Mohd Daud, Jamaluddin
Althunibat, Osama Yousef
author_sort Hashim, Ridzwan
title Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka
title_short Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka
title_full Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka
title_fullStr Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka
title_full_unstemmed Protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, H. scabra jaeger and stichopus horrens selenka
title_sort protein profiles of three species of malaysian sea cucumber; holothuria edulis lesson, h. scabra jaeger and stichopus horrens selenka
publisher EuroJournals Publishing, Inc.
publishDate 2012
url http://irep.iium.edu.my/25253/
http://irep.iium.edu.my/25253/
http://irep.iium.edu.my/25253/1/PROTEIN_PROFILES.pdf
first_indexed 2023-09-18T20:37:40Z
last_indexed 2023-09-18T20:37:40Z
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