Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.

Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.

Aging related reduction in cerebral blood flow (CBF) has been linked with neurodegenerative disorders including Alzheimer's diseases and dementia. Experimentally, a condition of chronic cerebral hypoperfusion due to reduced can be induced by permanent bilateral occlusion of common carotid arter...

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Main Authors: Saxena, Anil Kumar, Mohd. Fadly, Talib, Norlelawati A., Gurtu, Sunil
Format: Conference or Workshop Item
Language:English
Published: 2012
Subjects:
R Medicine (General)
RB Pathology
Online Access:http://irep.iium.edu.my/25255/
http://irep.iium.edu.my/25255/
http://irep.iium.edu.my/25255/1/O015.pdf
id iium-25255
recordtype eprints
spelling iium-252552013-08-01T02:55:26Z http://irep.iium.edu.my/25255/ Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats. Saxena, Anil Kumar Mohd. Fadly, Talib, Norlelawati A. Gurtu, Sunil R Medicine (General) RB Pathology Aging related reduction in cerebral blood flow (CBF) has been linked with neurodegenerative disorders including Alzheimer's diseases and dementia. Experimentally, a condition of chronic cerebral hypoperfusion due to reduced can be induced by permanent bilateral occlusion of common carotid arteries (2-vessel occlusion, 2VO) in rats. Since neuroinflammation, leading to neuronal apoptosis and death, is one of the mechanisms which is thought to play a significant role in chronic degenerative neurological disorders, the present study was planned to assess the neuroprotective role of ibuprofen in chronic cerebral hypoperfusion-induced neurodegeneration. Fifteen rats were equally divided into 3 groups. Group A - sham control, Group B - 2VO, and Group C - 2VO-I (treated daily with 50mg/kg orally ibuprofen following 2VO). After 8 weeks, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA1 region, hippocampal COX-2 mRNA expression and prostaglandin E2 (PGE-2) levels were estimated. There was a significant difference in neuronal cell death, increasing in COX-2 mRNA expression and PGE-2 levels in 2VO group compared to Sham control group. In ibuprofen treated 2VO rats the viable neuronal cell count of the hippocampal CA-1 region was significantly higher compared to the untreated 2VO group. For the hippocampal COX-2 mRNA expression, the value in the ibuprofen treated 2VO group showed no significant difference when compared to the untreated 2VO group. However, hippocampal PGE-2 levels were found to be significantly lower in ibuprofen treated 2VO rats compared to untreated 2Vo rats. Results indicate that ibuprofen is an effective neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/25255/1/O015.pdf Saxena, Anil Kumar and Mohd. Fadly, and Talib, Norlelawati A. and Gurtu, Sunil (2012) Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats. In: 26th Scientific Meeting of Malaysian Society of Pharmacology & Physiology, 18-20 may 2012, Equatorial Hotel, Penang. http://www.mspp2012.com/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic R Medicine (General)
RB Pathology
spellingShingle R Medicine (General)
RB Pathology
Saxena, Anil Kumar
Mohd. Fadly,
Talib, Norlelawati A.
Gurtu, Sunil
Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.
description Aging related reduction in cerebral blood flow (CBF) has been linked with neurodegenerative disorders including Alzheimer's diseases and dementia. Experimentally, a condition of chronic cerebral hypoperfusion due to reduced can be induced by permanent bilateral occlusion of common carotid arteries (2-vessel occlusion, 2VO) in rats. Since neuroinflammation, leading to neuronal apoptosis and death, is one of the mechanisms which is thought to play a significant role in chronic degenerative neurological disorders, the present study was planned to assess the neuroprotective role of ibuprofen in chronic cerebral hypoperfusion-induced neurodegeneration. Fifteen rats were equally divided into 3 groups. Group A - sham control, Group B - 2VO, and Group C - 2VO-I (treated daily with 50mg/kg orally ibuprofen following 2VO). After 8 weeks, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cell count in the hippocampal CA1 region, hippocampal COX-2 mRNA expression and prostaglandin E2 (PGE-2) levels were estimated. There was a significant difference in neuronal cell death, increasing in COX-2 mRNA expression and PGE-2 levels in 2VO group compared to Sham control group. In ibuprofen treated 2VO rats the viable neuronal cell count of the hippocampal CA-1 region was significantly higher compared to the untreated 2VO group. For the hippocampal COX-2 mRNA expression, the value in the ibuprofen treated 2VO group showed no significant difference when compared to the untreated 2VO group. However, hippocampal PGE-2 levels were found to be significantly lower in ibuprofen treated 2VO rats compared to untreated 2Vo rats. Results indicate that ibuprofen is an effective neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration.
format Conference or Workshop Item
author Saxena, Anil Kumar
Mohd. Fadly,
Talib, Norlelawati A.
Gurtu, Sunil
author_facet Saxena, Anil Kumar
Mohd. Fadly,
Talib, Norlelawati A.
Gurtu, Sunil
author_sort Saxena, Anil Kumar
title Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.
title_short Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.
title_full Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.
title_fullStr Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.
title_full_unstemmed Assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.
title_sort assessment of ibuprofen as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats.
publishDate 2012
url http://irep.iium.edu.my/25255/
http://irep.iium.edu.my/25255/
http://irep.iium.edu.my/25255/1/O015.pdf
first_indexed 2023-09-18T20:37:40Z
last_indexed 2023-09-18T20:37:40Z
_version_ 1777409154975006720

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