Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract

Moringa Oleifera seeds extract has been found to posses antimicrobial properties. The mechanism of its action in this regard is still largely un-determined. In this work, six methods of extracting the active ingredients from the Moringa seed powder were considered. They were: Normal aqueous extrac...

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Main Authors:	Bichi, Mustafa Hassan, Aguwamba, Jonah Chukwu, Muyibi, Suleyman Aremu, Abdulkarim, Mohd Ismail
Format:	Article
Language:	English
Published:	The Journal of American Science 2012
Subjects:	TD Environmental technology. Sanitary engineering TD201 Water supply for domestic and industrial purposes
Online Access:	http://irep.iium.edu.my/25909/ http://irep.iium.edu.my/25909/ http://irep.iium.edu.my/25909/1/Extraction-Bichi.pdf

id	iium-25909
recordtype	eprints
spelling	iium-259092012-10-02T00:24:16Z http://irep.iium.edu.my/25909/ Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract Bichi, Mustafa Hassan Aguwamba, Jonah Chukwu Muyibi, Suleyman Aremu Abdulkarim, Mohd Ismail TD Environmental technology. Sanitary engineering TD201 Water supply for domestic and industrial purposes Moringa Oleifera seeds extract has been found to posses antimicrobial properties. The mechanism of its action in this regard is still largely un-determined. In this work, six methods of extracting the active ingredients from the Moringa seed powder were considered. They were: Normal aqueous extraction (M1), Salt extraction (M2), Oil removal followed by aqueous extraction (M3), oil removal followed by salt extraction (M4), oil removal followed by aqueous extraction and microfiltration (M5), and oil removal followed by salt extraction and microfiltration. The antimicrobial action of each extract on Escherichia Coli was determined using Disc Diffusion and Agar Well methods. An average of 639.77±16.72gm of seed kernel was obtained per Kg of raw Moringa seed processed. The results also showed that 72.28±4.84% cake was produced for each 10gm of the <210 μm seed powder de-fatted and the seed oil produced was 28.75±1.68%. The zones of inhibition produced using disc diffusion method ranged 6.5mm – 9.0mm for the six extraction methods with the highest value of 9.0mm obtained with method M3. The Agar well method yielded 6.75mm – 12.38mm zone of inhibition diameter with the highest value of 12.38mm obtained with method M3 of seed extraction. The investigation thus concluded that the M3 was the best extraction method. The Minimum Inhibitory Concentration (MIC) for the Moringa disinfection Solution M3 was determined to be 200μL/mL while the Minimum Bacterial Concentration (MBC) was 210μL/mL. Optimization of the operating conditions was suggested for the subsequent application of this method of seed processing in water disinfection. The Journal of American Science 2012-09-25 Article PeerReviewed application/pdf en http://irep.iium.edu.my/25909/1/Extraction-Bichi.pdf Bichi, Mustafa Hassan and Aguwamba, Jonah Chukwu and Muyibi, Suleyman Aremu and Abdulkarim, Mohd Ismail (2012) Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract. Journal of American Science, 8 (9). pp. 450-458. ISSN 1545-1003 http://www.jofamericanscience.org/journals/am-sci/am0809/063_10571am0809_450_458.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TD Environmental technology. Sanitary engineering TD201 Water supply for domestic and industrial purposes
spellingShingle	TD Environmental technology. Sanitary engineering TD201 Water supply for domestic and industrial purposes Bichi, Mustafa Hassan Aguwamba, Jonah Chukwu Muyibi, Suleyman Aremu Abdulkarim, Mohd Ismail Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract
description	Moringa Oleifera seeds extract has been found to posses antimicrobial properties. The mechanism of its action in this regard is still largely un-determined. In this work, six methods of extracting the active ingredients from the Moringa seed powder were considered. They were: Normal aqueous extraction (M1), Salt extraction (M2), Oil removal followed by aqueous extraction (M3), oil removal followed by salt extraction (M4), oil removal followed by aqueous extraction and microfiltration (M5), and oil removal followed by salt extraction and microfiltration. The antimicrobial action of each extract on Escherichia Coli was determined using Disc Diffusion and Agar Well methods. An average of 639.77±16.72gm of seed kernel was obtained per Kg of raw Moringa seed processed. The results also showed that 72.28±4.84% cake was produced for each 10gm of the <210 μm seed powder de-fatted and the seed oil produced was 28.75±1.68%. The zones of inhibition produced using disc diffusion method ranged 6.5mm – 9.0mm for the six extraction methods with the highest value of 9.0mm obtained with method M3. The Agar well method yielded 6.75mm – 12.38mm zone of inhibition diameter with the highest value of 12.38mm obtained with method M3 of seed extraction. The investigation thus concluded that the M3 was the best extraction method. The Minimum Inhibitory Concentration (MIC) for the Moringa disinfection Solution M3 was determined to be 200μL/mL while the Minimum Bacterial Concentration (MBC) was 210μL/mL. Optimization of the operating conditions was suggested for the subsequent application of this method of seed processing in water disinfection.
format	Article
author	Bichi, Mustafa Hassan Aguwamba, Jonah Chukwu Muyibi, Suleyman Aremu Abdulkarim, Mohd Ismail
author_facet	Bichi, Mustafa Hassan Aguwamba, Jonah Chukwu Muyibi, Suleyman Aremu Abdulkarim, Mohd Ismail
author_sort	Bichi, Mustafa Hassan
title	Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract
title_short	Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract
title_full	Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract
title_fullStr	Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract
title_full_unstemmed	Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract
title_sort	effect of extraction method on the antimicrobial activity of moringa oleifera seeds extract
publisher	The Journal of American Science
publishDate	2012
url	http://irep.iium.edu.my/25909/ http://irep.iium.edu.my/25909/ http://irep.iium.edu.my/25909/1/Extraction-Bichi.pdf
first_indexed	2023-09-18T20:38:37Z
last_indexed	2023-09-18T20:38:37Z
_version_	1777409214079041536

Effect of extraction method on the antimicrobial activity of Moringa Oleifera seeds extract

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