Immbolization of lipase on multi-walled carbon nanotubes

Immobilized enzymes, such as lipases, have diverse applications including chemical, fuel, pharmaceutical and food processing industries. In this study, commercially available lipase extracted from Aspergillus niger has been immobilized on (MWNTs). The enzyme was bound to carbon nanotubes by covalent...

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Main Authors:	Alkhatib, Ma'an, Salleh, Hamzah, Sultan, Anans M.
Format:	Conference or Workshop Item
Language:	English
Published:	2008
Subjects:	TA401 Materials of engineering and construction TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/2613/ http://irep.iium.edu.my/2613/1/009_ALKHATIB_M.F._Malaysia.pdf

id	iium-2613
recordtype	eprints
spelling	iium-26132011-11-16T14:20:44Z http://irep.iium.edu.my/2613/ Immbolization of lipase on multi-walled carbon nanotubes Alkhatib, Ma'an Salleh, Hamzah Sultan, Anans M. TA401 Materials of engineering and construction TP248.13 Biotechnology Immobilized enzymes, such as lipases, have diverse applications including chemical, fuel, pharmaceutical and food processing industries. In this study, commercially available lipase extracted from Aspergillus niger has been immobilized on (MWNTs). The enzyme was bound to carbon nanotubes by covalent bonding, in the presence of N-(3-dimethylaminopropyl)-N'-ethyl)-carbodiimide (EDC) as a coupling reagent. Carbon nanotubes were carboxylated by sonication in 1 M nitric acid prior to treatment with the coupling reagent. Native lipase showed an activity of 2.22 x 10-3 U, whereas, immobilized lipase demonstrated an activity of 0.335 x 10-3, retaining 55 % of the native activity given that only 27.4 % enzyme immobilization has been achieved. One unit is defined as 1nmol of p-nitrophenol released by the hydrolysis of the substrate 4-nitrophenyl palmitate (pNPP) per 1 ml per minute. 2008 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/2613/1/009_ALKHATIB_M.F._Malaysia.pdf Alkhatib, Ma'an and Salleh, Hamzah and Sultan, Anans M. (2008) Immbolization of lipase on multi-walled carbon nanotubes. In: 2008 International Conference on Environment (ICENV 2008), 15-17 December, 2008, G Hotel, Penang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA401 Materials of engineering and construction TP248.13 Biotechnology
spellingShingle	TA401 Materials of engineering and construction TP248.13 Biotechnology Alkhatib, Ma'an Salleh, Hamzah Sultan, Anans M. Immbolization of lipase on multi-walled carbon nanotubes
description	Immobilized enzymes, such as lipases, have diverse applications including chemical, fuel, pharmaceutical and food processing industries. In this study, commercially available lipase extracted from Aspergillus niger has been immobilized on (MWNTs). The enzyme was bound to carbon nanotubes by covalent bonding, in the presence of N-(3-dimethylaminopropyl)-N'-ethyl)-carbodiimide (EDC) as a coupling reagent. Carbon nanotubes were carboxylated by sonication in 1 M nitric acid prior to treatment with the coupling reagent. Native lipase showed an activity of 2.22 x 10-3 U, whereas, immobilized lipase demonstrated an activity of 0.335 x 10-3, retaining 55 % of the native activity given that only 27.4 % enzyme immobilization has been achieved. One unit is defined as 1nmol of p-nitrophenol released by the hydrolysis of the substrate 4-nitrophenyl palmitate (pNPP) per 1 ml per minute.
format	Conference or Workshop Item
author	Alkhatib, Ma'an Salleh, Hamzah Sultan, Anans M.
author_facet	Alkhatib, Ma'an Salleh, Hamzah Sultan, Anans M.
author_sort	Alkhatib, Ma'an
title	Immbolization of lipase on multi-walled carbon nanotubes
title_short	Immbolization of lipase on multi-walled carbon nanotubes
title_full	Immbolization of lipase on multi-walled carbon nanotubes
title_fullStr	Immbolization of lipase on multi-walled carbon nanotubes
title_full_unstemmed	Immbolization of lipase on multi-walled carbon nanotubes
title_sort	immbolization of lipase on multi-walled carbon nanotubes
publishDate	2008
url	http://irep.iium.edu.my/2613/ http://irep.iium.edu.my/2613/1/009_ALKHATIB_M.F._Malaysia.pdf
first_indexed	2023-09-18T20:10:13Z
last_indexed	2023-09-18T20:10:13Z
_version_	1777407427190194176

Immbolization of lipase on multi-walled carbon nanotubes

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