Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development

We earlier reported proteomic profiles indicating VDAC2 as a potential biomarker for over-stunned zchicken. The current work focused on cloning VDAC2 gene from chicken’s skeletal muscle into Escherichia coli. R A from chicken’s skeletal muscle was isolated and purified to synthesize the cDNA. Aft...

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Main Authors:	Ahmad Hassan, Nurhidayah, Amid, Azura, Mohd Salleh, Hamzah
Format:	Conference or Workshop Item
Language:	English English
Published:	2012
Subjects:	S Agriculture (General)
Online Access:	http://irep.iium.edu.my/26146/ http://irep.iium.edu.my/26146/ http://irep.iium.edu.my/26146/1/BT-203_Nur_Hidayah_Hassan_Micotribe_2012.pdf http://irep.iium.edu.my/26146/4/BT203.pdf

id	iium-26146
recordtype	eprints
spelling	iium-261462014-07-17T02:20:25Z http://irep.iium.edu.my/26146/ Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development Ahmad Hassan, Nurhidayah Amid, Azura Mohd Salleh, Hamzah S Agriculture (General) We earlier reported proteomic profiles indicating VDAC2 as a potential biomarker for over-stunned zchicken. The current work focused on cloning VDAC2 gene from chicken’s skeletal muscle into Escherichia coli. R A from chicken’s skeletal muscle was isolated and purified to synthesize the cDNA. After DNA amplification by proofread DNA polymerase, the PCR product was purified and inserted into pENTR-TEV-D-TOPO entry vector and transformed in One Shot Chemically Competent E. coli cells. The entry clone then were inserted into pDEST 17 cloning vector using LR recombination reaction and E.coli DH5α strain as the host. The vector construct was tranformed into E. coli BL21A1 strain for protein expression. All positive clones were identified by PCR and restriction enzyme digestion as well validated by DNA sequencing. Recombinant VDAC2 protein was purified by Ni-NTA spin-column and Western blot performed on the purified recombinant VDAC2 protein with anti-His followed by AP goat anti-rabbit revealed a 30 kDa protein band and confirmed the protein expression. 2012 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/26146/1/BT-203_Nur_Hidayah_Hassan_Micotribe_2012.pdf application/pdf en http://irep.iium.edu.my/26146/4/BT203.pdf Ahmad Hassan, Nurhidayah and Amid, Azura and Mohd Salleh, Hamzah (2012) Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development. In: Malaysian International Conference on Trends in Bioprocess Engineering (MICOTriBE), 3-5 July 2012, Langkawi, Kedah, Malaysia. (Unpublished) http://micotribe2012.unimap.edu.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	S Agriculture (General)
spellingShingle	S Agriculture (General) Ahmad Hassan, Nurhidayah Amid, Azura Mohd Salleh, Hamzah Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development
description	We earlier reported proteomic profiles indicating VDAC2 as a potential biomarker for over-stunned zchicken. The current work focused on cloning VDAC2 gene from chicken’s skeletal muscle into Escherichia coli. R A from chicken’s skeletal muscle was isolated and purified to synthesize the cDNA. After DNA amplification by proofread DNA polymerase, the PCR product was purified and inserted into pENTR-TEV-D-TOPO entry vector and transformed in One Shot Chemically Competent E. coli cells. The entry clone then were inserted into pDEST 17 cloning vector using LR recombination reaction and E.coli DH5α strain as the host. The vector construct was tranformed into E. coli BL21A1 strain for protein expression. All positive clones were identified by PCR and restriction enzyme digestion as well validated by DNA sequencing. Recombinant VDAC2 protein was purified by Ni-NTA spin-column and Western blot performed on the purified recombinant VDAC2 protein with anti-His followed by AP goat anti-rabbit revealed a 30 kDa protein band and confirmed the protein expression.
format	Conference or Workshop Item
author	Ahmad Hassan, Nurhidayah Amid, Azura Mohd Salleh, Hamzah
author_facet	Ahmad Hassan, Nurhidayah Amid, Azura Mohd Salleh, Hamzah
author_sort	Ahmad Hassan, Nurhidayah
title	Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development
title_short	Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development
title_full	Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development
title_fullStr	Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development
title_full_unstemmed	Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development
title_sort	molecular cloning and expression of voltagedependent anion channel 2 (vdac2) from electrically stimulated chicken for antibody development
publishDate	2012
url	http://irep.iium.edu.my/26146/ http://irep.iium.edu.my/26146/ http://irep.iium.edu.my/26146/1/BT-203_Nur_Hidayah_Hassan_Micotribe_2012.pdf http://irep.iium.edu.my/26146/4/BT203.pdf
first_indexed	2023-09-18T20:38:59Z
last_indexed	2023-09-18T20:38:59Z
_version_	1777409237134082048

Molecular cloning and expression of voltagedependent Anion Channel 2 (VDAC2) from electrically stimulated chicken for antibody development

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