Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture

The suitability of solid phase toxin tracking (SPATT) for the detection of dissolved ciguatoxins (CTXs) and maitotoxins (MTXs) was evaluated under in vitro conditions for its possible application to toxin evaluation in Gambierdiscus spp. cultures and in the field. Pacific type 1 CTX (CTX1B) and MTX...

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Main Authors:	Caillaud, Amandine, de la Iglesia, Pablo, Barber, Esther, Eixarch, Helena, Mohammad Noor, Normawaty, Yasumoto, Takeshi, Dioge`ne, Jorge
Format:	Article
Language:	English
Published:	Elsevier 2011
Subjects:	QD Chemistry SH Aquaculture. Fisheries. Angling
Online Access:	http://irep.iium.edu.my/2630/ http://irep.iium.edu.my/2630/ http://irep.iium.edu.my/2630/ http://irep.iium.edu.my/2630/1/gambierdiscus_pacificus_toxin.PDF

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recordtype	eprints
spelling	iium-26302017-06-15T06:45:14Z http://irep.iium.edu.my/2630/ Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture Caillaud, Amandine de la Iglesia, Pablo Barber, Esther Eixarch, Helena Mohammad Noor, Normawaty Yasumoto, Takeshi Dioge`ne, Jorge QD Chemistry SH Aquaculture. Fisheries. Angling The suitability of solid phase toxin tracking (SPATT) for the detection of dissolved ciguatoxins (CTXs) and maitotoxins (MTXs) was evaluated under in vitro conditions for its possible application to toxin evaluation in Gambierdiscus spp. cultures and in the field. Pacific type 1 CTX (CTX1B) and MTX standards tracked by the DIAON1 HP20 resin were evaluated using two Neuroblastoma cell based-assays (Neuro- 2a CBA) specifically conceived for these two toxins. Recoveries were estimated at 90.9 (�6.4), 85.5 (�13.2) and 89 (�11.3)% for dissolved CTX1B after respectively 24, 72 and 120-h exposure, and 66.2 (�11.9)% for dissolved MTX after 72-h exposure. The approach was further applied for the detection of dissolved toxins in the culture medium of Gambierdiscus pacificus with tentative confirmation of the identity of CTXs congeners using liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis. Both the Neuro-2a CBA for CTXs and LC–MS/MS analysis allowed the identification and quantification of dissolved CTX-like compounds from day 16 to 47 of the culture, being the congeners 51-hydroxyCTX-3 C and 2,3-dihydroxyCTX- 3C determined as the most abundant by LC–MS/MS. The MTX-like toxicity was detected from day 19 to 47 (final of exponential to decade growth phases). Dissolved CTX- and MTX-like toxicity evaluated with SPATT devices and intracellular CTX- and MTX-like toxicity evaluated in harvested G. pacificus cells from culture were analyzed to investigate possible physiological variations of toxin production between the different growth phases of G. pacificus cultures. These results confirmed for the first time the suitability of SPATT devices for recovering dissolved CTX1B and MTX under in vitro conditions. The use of SPATT devices may be considered as a useful approach to monitor the incidence and prevalence of CTX in ciguatera endemic areas,as well as to increase the knowledge on CTX- and MTX-producing species. Elsevier 2011-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/2630/1/gambierdiscus_pacificus_toxin.PDF Caillaud, Amandine and de la Iglesia, Pablo and Barber, Esther and Eixarch, Helena and Mohammad Noor, Normawaty and Yasumoto, Takeshi and Dioge`ne, Jorge (2011) Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture. Harmful Algae, 10 (5). pp. 433-446. ISSN 1568-9883 http://www.elsevier.com/locate/hal DOI: 10.1016/j.hal.2011.02.004
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry SH Aquaculture. Fisheries. Angling
spellingShingle	QD Chemistry SH Aquaculture. Fisheries. Angling Caillaud, Amandine de la Iglesia, Pablo Barber, Esther Eixarch, Helena Mohammad Noor, Normawaty Yasumoto, Takeshi Dioge`ne, Jorge Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture
description	The suitability of solid phase toxin tracking (SPATT) for the detection of dissolved ciguatoxins (CTXs) and maitotoxins (MTXs) was evaluated under in vitro conditions for its possible application to toxin evaluation in Gambierdiscus spp. cultures and in the field. Pacific type 1 CTX (CTX1B) and MTX standards tracked by the DIAON1 HP20 resin were evaluated using two Neuroblastoma cell based-assays (Neuro- 2a CBA) specifically conceived for these two toxins. Recoveries were estimated at 90.9 (�6.4), 85.5 (�13.2) and 89 (�11.3)% for dissolved CTX1B after respectively 24, 72 and 120-h exposure, and 66.2 (�11.9)% for dissolved MTX after 72-h exposure. The approach was further applied for the detection of dissolved toxins in the culture medium of Gambierdiscus pacificus with tentative confirmation of the identity of CTXs congeners using liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis. Both the Neuro-2a CBA for CTXs and LC–MS/MS analysis allowed the identification and quantification of dissolved CTX-like compounds from day 16 to 47 of the culture, being the congeners 51-hydroxyCTX-3 C and 2,3-dihydroxyCTX- 3C determined as the most abundant by LC–MS/MS. The MTX-like toxicity was detected from day 19 to 47 (final of exponential to decade growth phases). Dissolved CTX- and MTX-like toxicity evaluated with SPATT devices and intracellular CTX- and MTX-like toxicity evaluated in harvested G. pacificus cells from culture were analyzed to investigate possible physiological variations of toxin production between the different growth phases of G. pacificus cultures. These results confirmed for the first time the suitability of SPATT devices for recovering dissolved CTX1B and MTX under in vitro conditions. The use of SPATT devices may be considered as a useful approach to monitor the incidence and prevalence of CTX in ciguatera endemic areas,as well as to increase the knowledge on CTX- and MTX-producing species.
format	Article
author	Caillaud, Amandine de la Iglesia, Pablo Barber, Esther Eixarch, Helena Mohammad Noor, Normawaty Yasumoto, Takeshi Dioge`ne, Jorge
author_facet	Caillaud, Amandine de la Iglesia, Pablo Barber, Esther Eixarch, Helena Mohammad Noor, Normawaty Yasumoto, Takeshi Dioge`ne, Jorge
author_sort	Caillaud, Amandine
title	Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture
title_short	Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture
title_full	Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture
title_fullStr	Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture
title_full_unstemmed	Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture
title_sort	monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to gambierdiscus pacificus in culture
publisher	Elsevier
publishDate	2011
url	http://irep.iium.edu.my/2630/ http://irep.iium.edu.my/2630/ http://irep.iium.edu.my/2630/ http://irep.iium.edu.my/2630/1/gambierdiscus_pacificus_toxin.PDF
first_indexed	2023-09-18T20:10:15Z
last_indexed	2023-09-18T20:10:15Z
_version_	1777407429235965952

Monitoring of dissolved ciguatoxin and maitotoxin using solid-phase adsorption toxin tracking devices: application to Gambierdiscus pacificus in culture

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