Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects.

Mixed-genotypes hepatitis C virus (HCV) infections are normally ignored in chronic hemodialysis patients. The aim of this study is to investigate the prevalence of mixed-genotypes infections among hemodialysis patients in Pahang province, Malaysia. Reverse-transcription and polymerase chain reaction...

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Main Authors:	Hamzah, Hairul Aini, Mustafa Mahmoud, Mohammed Imad Al-Deen, MR, Seman, BA, Nasuruddin
Format:	Article
Language:	English
Published:	Malaysian Medical Association 2012
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/27167/ http://irep.iium.edu.my/27167/1/14-Mixed-genotypes%28r2%29.pdf

id	iium-27167
recordtype	eprints
spelling	iium-271672012-11-29T06:54:14Z http://irep.iium.edu.my/27167/ Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects. Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen MR, Seman BA, Nasuruddin R Medicine (General) Mixed-genotypes hepatitis C virus (HCV) infections are normally ignored in chronic hemodialysis patients. The aim of this study is to investigate the prevalence of mixed-genotypes infections among hemodialysis patients in Pahang province, Malaysia. Reverse-transcription and polymerase chain reaction methods were performed using two different sets of primers, targeting the 5' untranslated region and nonstructural 5B region. Target region base sequences were obtained by direct sequencing. Discrepancy in outcomes from phylogenetic analysis of both regions suggests double infections. Of 40 subjects in eight hemodialysis centres, evidence of mixed-genotypes infections was found in 5 subjects (12.5%) from three different centres. Four patients were infected with mixed genotypes 3 and 1 and one with genotypes 3 and 4. Cases of mixed HCV genotypes infection were considered high among hemodialysis patients in Pahang. However, further investigation is needed to confirm whether they are true mixed infections or perhaps infection with recombinant virus and also to assess the clinicopathologic characteristics of the infection. Malaysian Medical Association 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/27167/1/14-Mixed-genotypes%28r2%29.pdf Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and MR, Seman and BA, Nasuruddin (2012) Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects. The Medical Journal of Malaysia, 67 (2). pp. 199-203. ISSN 0300-5283
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen MR, Seman BA, Nasuruddin Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects.
description	Mixed-genotypes hepatitis C virus (HCV) infections are normally ignored in chronic hemodialysis patients. The aim of this study is to investigate the prevalence of mixed-genotypes infections among hemodialysis patients in Pahang province, Malaysia. Reverse-transcription and polymerase chain reaction methods were performed using two different sets of primers, targeting the 5' untranslated region and nonstructural 5B region. Target region base sequences were obtained by direct sequencing. Discrepancy in outcomes from phylogenetic analysis of both regions suggests double infections. Of 40 subjects in eight hemodialysis centres, evidence of mixed-genotypes infections was found in 5 subjects (12.5%) from three different centres. Four patients were infected with mixed genotypes 3 and 1 and one with genotypes 3 and 4. Cases of mixed HCV genotypes infection were considered high among hemodialysis patients in Pahang. However, further investigation is needed to confirm whether they are true mixed infections or perhaps infection with recombinant virus and also to assess the clinicopathologic characteristics of the infection.
format	Article
author	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen MR, Seman BA, Nasuruddin
author_facet	Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen MR, Seman BA, Nasuruddin
author_sort	Hamzah, Hairul Aini
title	Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects.
title_short	Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects.
title_full	Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects.
title_fullStr	Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects.
title_full_unstemmed	Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects.
title_sort	mixed-genotypes infections with hepatitis c virus in hemodialysis subjects.
publisher	Malaysian Medical Association
publishDate	2012
url	http://irep.iium.edu.my/27167/ http://irep.iium.edu.my/27167/1/14-Mixed-genotypes%28r2%29.pdf
first_indexed	2023-09-18T20:40:23Z
last_indexed	2023-09-18T20:40:23Z
_version_	1777409325639139328

Mixed-genotypes infections with hepatitis C virus in hemodialysis subjects.

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